FastQCFastQC Report
Fri 14 Jul 2023
SRR11626664_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11626664_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47312387
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG39052398.254157626838824TruSeq Adapter, Index 7 (100% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG23595174.987102003540849Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG13410202.8343951447640974Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA11777262.4892550866224528Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA10711672.2640307706309555Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT7969931.6845334816863078Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG7765191.6412594021096418Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG7715421.6307399582270072Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG7163001.513979837880511No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT7155011.5122910623807673No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT7141261.5093848467210078Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA6114081.292278912074337Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA5171021.0929526764312272Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA4368300.9232888630201642Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA4173590.8821347356665813Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3883970.820920322620797No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA3136300.6628919399057165Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA2702990.5713070448125984Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT2473690.5228419356647551No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC2346530.4959652532432997Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT2181330.46104839309840784Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGAA1936900.4093853899191347Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACGTCT1835350.387921666264693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1706550.3606983515754553Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCT1677610.35458156021593246Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGA1674840.35399608986120273Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTG1637880.3461841821677693Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGTC1586440.33531176518318556No Hit
CGACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAA1559010.329514129143389Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA1516260.32047844045577323Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCAGATCGGAAGAGCACACG1497640.3165428960496117No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCAGATCGGAAGAGCACACGTCTG1446890.30581631825086314Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGACGAAAAATAACAATACAGGAAGATCGGAAGAGCACACGTCTGAAC1321780.2793729261641354Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGA1038100.2194139982833671Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA1016980.21495005102997655Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACA1014070.21433499011580204No Hit
TGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTGA924080.19531460122694716Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG920190.19449240639665888Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTC901470.1905357258766082No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT896870.18956346463770682No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCT895790.1893351946077039Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGAGATCGGAAGAGCACACGTCT893240.18879622370353033Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCAGATCGGAAGAGCACACGTC825680.17451666516001402No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGAGATCGGAAGAGCACACG802950.169712426473008No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGT756780.15995388269038296No Hit
ACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAA756320.15985665656649284Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC744390.15733511817951606No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG734530.15525109735004494No Hit
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC721450.15248649365334285Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG679620.14364525721350732Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC658180.13911367439567146TruSeq Adapter, Index 7 (100% over 50bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC622540.13158076340557495No Hit
GGACCGGCGCAAGACGGACCAGAGCGAAAGCAGATCGGAAGAGCACACGT608810.12867877496859334No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA599380.12668563942884556No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG587250.12412182881409049No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG575810.12170385738517062No Hit
GGGAGGGCGCGCGGGTCGGGGCGGCGGCAGATCGGAAGAGCACACGTCTG527130.11141479714392764Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT524480.11085469012586492Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGTCT508500.1074771391263772Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC506490.10705230323720509No Hit
CGACCTGGTATTGCAGTACTTCCAGGAGATCGGAAGAGCACACGTCTGAA490840.1037445014135516Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT483270.10214449759214221Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTC478770.10119337246713002No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)