FastQCFastQC Report
Wed 30 Aug 2023
SRR11685403_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11685403_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32623930
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGC9962253.053663369189426No Hit
TCGGAGATAGGGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG7019972.1517855144981004No Hit
TGGAGAATTGTAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4601681.4105228891798138No Hit
GGCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGAG3679571.1278745387205036No Hit
AACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGA3556991.0903008926269766No Hit
CCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGAG2936080.8999774092207775No Hit
AGGAGTGTGTAACAACTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC2505640.7680374498106145No Hit
CGGAGATAGGGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2459770.7539772185631836No Hit
TCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGAG2273950.697019028670059No Hit
AAATGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAG2214420.6787716869181609No Hit
AACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAA2201080.6746826639218513No Hit
TGGCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGA2116280.648689474260152No Hit
AAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGA1835630.5626636643715212No Hit
TTGTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAG1564400.47952530550427247No Hit
AAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCGG1483890.45484710149880775No Hit
ATGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAGAG1422830.4361307788485323No Hit
AAGTGGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1397910.4284922141507783Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTCAGCCTGCTAAATAGTCAGCATCGCACTGTAGGCACCATCAATAGA1359450.41670332176411606No Hit
AGGATGTTGACACAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1322190.405282257533044No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG1317250.4037680316258648No Hit
GCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGAGC1217060.37305744586872275No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCG1193640.3658786663654563No Hit
TTGCGGTGCGCCGACTTCTTAGAGGGCTGTAGGCACCATCAATAGATCGG1091240.33449066375510245No Hit
ACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGAA1052420.32259142292176324No Hit
TGGAGAATTGTAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1022710.31348461083627877No Hit
TCAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCG1015470.31126538096421863No Hit
TGGCCCTGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1015200.31118261962921084Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGG1013210.3105726379378573No Hit
ACGGTGGTCATGGAAGTCGAAATCCGCTCTGTAGGCACCATCAATAGATC974100.2985845053002505No Hit
TTAGCCGAGATATACTAAAGGCCGTCTGTAGGCACCATCAATAGATCGGA967210.29647255864023736No Hit
CCGACTAAGGATACATCCGCCTGTAGGCACCATCAATAGATCGGAAGAGC936160.286955005114344No Hit
TCGGAGATAGGGCTCACTGTAGGCACCATCAATAGATCGGAAGAGCACAC911860.2795064849636448No Hit
TCGGGAATAGCCCAACTTGAAAATCTCCCTGTAGGCACCATCAATAGATC904030.27710640624841953No Hit
TTGTCCTAAGATGAGCTCAACGAGAACCTGTAGGCACCATCAATAGATCG897820.27520289554324084No Hit
TCAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATC884070.27098819792710443No Hit
TGTGCGGATGCTGGAGCATACCTGTAGGCACCATCAATAGATCGGAAGAG818460.2508771935202166No Hit
TTCAGTTATAGTTTATTTGATGCTGTAGGCACCATCAATAGATCGGAAGA804970.2467421920044581No Hit
TGCGGTGCGCCGACTTCTTAGAGGGCTGTAGGCACCATCAATAGATCGGA789230.24191751269696812No Hit
TGATGGTAGGATAGAGGCCTACCATCTGTAGGCACCATCAATAGATCGGA760940.23324596392893193No Hit
TAGCCGAGATATACTAAAGGCCGTCTGTAGGCACCATCAATAGATCGGAA750620.23008264179085722No Hit
TGACATGCCAATTGTGTACCAGTCTGTAGGCACCATCAATAGATCGGAAG719470.22053443591866462No Hit
AATGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAGA717240.21985088859619306No Hit
TAAGTGGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC714600.21904166665389488No Hit
ATCAGTTATAGTTTATTTGATGCTGTAGGCACCATCAATAGATCGGAAGA712850.21850525059365933No Hit
TGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGC711000.21793818218712463No Hit
TACACTGACGCGACCAACGCTGTAGGCACCATCAATAGATCGGAAGAGCA708640.2172147868144641No Hit
ACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAG693120.21245754266883238No Hit
TGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGCAC690540.2116667121343137No Hit
TCGACTAAGGATACATCCGCCTGTAGGCACCATCAATAGATCGGAAGAGC678930.2081079747289796No Hit
CCGGGAATAGCCCAACTTGAAAATCTCCCTGTAGGCACCATCAATAGATC678590.2080037567515624No Hit
CGCTTCCATGAGTAGGAGGGCCTGTAGGCACCATCAATAGATCGGAAGAG674580.2067745976649656No Hit
TGTGCGGATGCTGGAGCATACCCTGTAGGCACCATCAATAGATCGGAAGA668000.20475767327847996No Hit
ACGGAGGCGTGCAAAGGTTCCCCTGTAGGCACCATCAATAGATCGGAAGA662620.2031085770475844No Hit
ACCGGGAATAGCCCAACTTGAAAATCTCCCTGTAGGCACCATCAATAGAT629530.19296571565718784No Hit
AGGCCCTGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG622390.19077713813142683Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCCCATTAAAGTGGTACGTGAGCTGGGCTGTAGGCACCATCAATAGAT613910.18817781916525692No Hit
TTGCGGTGCGCCGACTTCTTAGAGGCTGTAGGCACCATCAATAGATCGGA611980.18758622888168286No Hit
TAAACTCAAGATGAGTGCTCTCCTGTAGGCACCATCAATAGATCGGAAGA601490.18437079775489956No Hit
TCGCCCATTAAAGTGGTACGTGAGCTGGGTCTGTAGGCACCATCAATAGA598460.18344203166203457No Hit
ACGGAGGCGTGCAAAGGTTCCCTCTGTAGGCACCATCAATAGATCGGAAG591940.18144349868332846No Hit
TGGCGGTAACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT581120.1781269148137579Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGAGGCGTGCAAAGGTTCCCTCTGTAGGCACCATCAATAGATCGGAAGA580510.1779399355013329No Hit
TAACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGA580260.177863304635585No Hit
TACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGAG567890.17407160939837718No Hit
ACGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCG534460.16382453003056346No Hit
CCGATCTTGCCAAGTCGTACTCCTGTAGGCACCATCAATAGATCGGAAGA532810.1633187663166271No Hit
TGGACATGCCAATTGTGTACCAGTCTGTAGGCACCATCAATAGATCGGAA531300.16285591588750958No Hit
CTGAAAGGACATGCCAATTGTGTACCAGTCTGTAGGCACCATCAATAGAT525560.161096471209937No Hit
GCGAAGAACCTTACCAGGGCTGTAGGCACCATCAATAGATCGGAAGAGCA524390.16073783875823666No Hit
TCGACGCTTCCATGAGTAGGAGGGCCTGTAGGCACCATCAATAGATCGGA499070.1529766646752859No Hit
ACCCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAG498490.15279888106675069No Hit
TGAACTGTCTTGGGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACA490550.1503650847705963No Hit
TTACCCGCTGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT476740.14613199574668043Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGAAGAG476620.14609521293112143No Hit
TGCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGAG475470.1457427109486809No Hit
CAAGCGGTGGATTATGTGGATTAATTCGCTGTAGGCACCATCAATAGATC468030.1434621763840224No Hit
TGCGGTGCGCCGACTTCTTAGAGGCTGTAGGCACCATCAATAGATCGGAA466810.1430882177591725No Hit
CGATGGTAGGATAGAGGCCTACCATCTGTAGGCACCATCAATAGATCGGA466800.14308515252454257No Hit
CTGGCGGTAACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC466270.14292269508915695No Hit
CTGGCGGTAACGCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT462260.1416935360025601No Hit
AAAACTCAAGATGAGTGCTCTCCTGTAGGCACCATCAATAGATCGGAAGA445940.13669107308653494No Hit
TCCACGAGTCGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC445250.13647957189707066No Hit
GCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCGGAAGA442650.13568261089329212No Hit
CCGATCTTGCCAAGTCGTACTGTAGGCACCATCAATAGATCGGAAGAGCA441660.1353791526649303No Hit
CGGAGGCGTGCAAAGGTTCCCCTGTAGGCACCATCAATAGATCGGAAGAG435330.13343885914419262No Hit
GGCGACGGTGAAATACCACTACCTGTAGGCACCATCAATAGATCGGAAGA432550.1325867239170756No Hit
TGTGCGGATGCTGGAGCATACCCCTGTAGGCACCATCAATAGATCGGAAG408080.1250860947776678No Hit
TCCCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAG405650.12434124276259788No Hit
CGGTGAATCATGATAACTTCTGTAGGCACCATCAATAGATCGGAAGAGCA403590.1237098044288349No Hit
TAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG399600.12248677581149787No Hit
ATCCCGAGTAGCATGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC393500.12061698268724828No Hit
GCGGTGAATCATGATAACTTCCTGTAGGCACCATCAATAGATCGGAAGAG384240.11777857541994481No Hit
GCGGTGAATCATGATAACTTCTGTAGGCACCATCAATAGATCGGAAGAGC383850.11765903126937803No Hit
TCGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCG372610.11421370754535091No Hit
AAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG372160.11407577198700462No Hit
TCCTGAAAGGACATGCCAATTGTGTACCAGTCTGTAGGCACCATCAATAG369280.11319298441358842No Hit
TATGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAGA369160.11315620159802942No Hit
GCGAAGAACCTTACCAGGGTCTGTAGGCACCATCAATAGATCGGAAGAGC368450.11293856993930529No Hit
ACCACGTGCGAACCCTCAGTGACGATCTGTCTGTAGGCACCATCAATAGA353160.10825182619016165No Hit
CGGGAATAGCCCAACTTGAAAATCTCCCTGTAGGCACCATCAATAGATCG350770.10751923511361139No Hit
CGATGGTAGGATAGAGGCCTACCATGCTGTAGGCACCATCAATAGATCGG330230.10122324318376114No Hit
AGGACATGCCAATTGTGTACCAGTCTGTAGGCACCATCAATAGATCGGAA329820.10109756856393451No Hit
GACATGCCAATTGTGTACCAGTCTGTAGGCACCATCAATAGATCGGAAGA329810.10109450332930459No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)