FastQCFastQC Report
Wed 30 Aug 2023
SRR11685404_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11685404_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26695245
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGC10029223.756931243747716No Hit
CCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGAG4264231.5973743638614293No Hit
AACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAA3482181.304419569852234No Hit
TCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGAG3417001.2800032365314498No Hit
AACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGA3369761.262307201151366No Hit
AGGAGTGTGTAACAACTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC3041131.1392028805129901No Hit
GGCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGAG2940111.1013609352527014No Hit
GCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGAGC2813731.0540191708298614No Hit
ATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1809820.6779559430902394Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TACACTGACGCGACCAACGCTGTAGGCACCATCAATAGATCGGAAGAGCA1616600.6055760117578992No Hit
ACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAG1517490.5684495497231811No Hit
CCGACTAAGGATACATCCGCCTGTAGGCACCATCAATAGATCGGAAGAGC1475410.5526864428477806No Hit
TGGCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGA1442680.5404258323907497No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1376990.5158184538107816Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAG1375020.5150804946723658No Hit
ACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGAA1366380.5118439632226638No Hit
TGATGGTAGGATAGAGGCCTACCATCTGTAGGCACCATCAATAGATCGGA1295640.4853448619782287No Hit
AAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGA1283620.4808421874382498No Hit
AAATGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAG1273940.4772160734992318No Hit
TCGGAGATAGGGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1270220.47582256690283226No Hit
TGCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGAG1168910.4378719880637919No Hit
ATGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAGAG1165340.43653467124950535No Hit
TGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGCAC1060270.39717560187216866No Hit
TACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGAG1043160.39076622072582595No Hit
TCGACTAAGGATACATCCGCCTGTAGGCACCATCAATAGATCGGAAGAGC1042790.39062761926328077No Hit
AAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCGG822650.3081634950344153No Hit
AGGATGTTGACACAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACA794380.29757359409887413No Hit
TGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGC780900.29252400567966313No Hit
TGTGCGGATGCTGGAGCATACCTGTAGGCACCATCAATAGATCGGAAGAG779050.29183099836693766No Hit
TAACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGG771850.2891338888255193No Hit
TCCACGAGTCGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC762240.28553399678482067No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG741550.27778355283871714No Hit
TAACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGA704590.2639383905261031No Hit
TTCAGTTATAGTTTATTTGATGCTGTAGGCACCATCAATAGATCGGAAGA687540.2575514852926055No Hit
TTGCGGTGCGCCGACTTCTTAGAGGGCTGTAGGCACCATCAATAGATCGG661550.24781566904518013No Hit
TGGAGAATTGTAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT654440.24515227337302956No Hit
CGGTGAATCATGATAACTTCTGTAGGCACCATCAATAGATCGGAAGAGCA649970.24347781786606565No Hit
ATCAGTTATAGTTTATTTGATGCTGTAGGCACCATCAATAGATCGGAAGA645960.24197567769091463No Hit
CCGATCTTGCCAAGTCGTACTGTAGGCACCATCAATAGATCGGAAGAGCA644610.24146996965189868No Hit
AATGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAGA628850.23556629654457187No Hit
AGGATGTTGACACAATGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC623960.23373450964769196No Hit
GCGAAGAACCTTACCAGGGTCTGTAGGCACCATCAATAGATCGGAAGAGC620880.23258074612164076No Hit
TAGCCGAGATATACTAAAGGCCGTCTGTAGGCACCATCAATAGATCGGAA612200.22932923073004202No Hit
TGCGGTGCGCCGACTTCTTAGAGGGCTGTAGGCACCATCAATAGATCGGA604970.2266208832322011No Hit
AAAACTCAAGATGAGTGCTCTCCTGTAGGCACCATCAATAGATCGGAAGA602250.2256019751832208No Hit
TTGTCCTAAGATGAGCTCAACGAGAACCTGTAGGCACCATCAATAGATCG583910.21873183782355246No Hit
GCGAAGAACCTTACCAGGGCTGTAGGCACCATCAATAGATCGGAAGAGCA575900.2157313034587246No Hit
ACCCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAG557560.2088611660990562No Hit
GTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGCA553640.2073927397931729No Hit
GCGGTGAATCATGATAACTTCTGTAGGCACCATCAATAGATCGGAAGAGC551200.20647871933747003No Hit
TCGGGAATAGCCCAACTTGAAAATCTCCCTGTAGGCACCATCAATAGATC542830.2033433294955712No Hit
CCCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGA520130.19483994246915506No Hit
GCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGAAGAG519690.19467511910829063No Hit
TAAACTCAAGATGAGTGCTCTCCTGTAGGCACCATCAATAGATCGGAAGA517460.1938397643475458No Hit
TTAGCCGAGATATACTAAAGGCCGTCTGTAGGCACCATCAATAGATCGGA501580.1878911394145287No Hit
CCGGGAATAGCCCAACTTGAAAATCTCCCTGTAGGCACCATCAATAGATC493590.1848980970206492No Hit
TCTACACTGACGCGACCAACGCTGTAGGCACCATCAATAGATCGGAAGAG492120.18434743715594293No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCG487530.18262802982328877No Hit
CGATGGTAGGATAGAGGCCTACCATCTGTAGGCACCATCAATAGATCGGA477830.1789944239133224No Hit
TCCCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAG472640.17705025745221667No Hit
ACCTCAGCCTGCTAAATAGTCAGCATCGCACTGTAGGCACCATCAATAGA448550.16802617844488785No Hit
TGTGCGGATGCTGGAGCATACCCTGTAGGCACCATCAATAGATCGGAAGA443140.1659996003033499No Hit
GCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGAG435830.16326128492171546No Hit
TCAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCG420930.1576797665651692No Hit
ACGGAGGCGTGCAAAGGTTCCCCTGTAGGCACCATCAATAGATCGGAAGA407790.15275754165208075No Hit
ATCCCGAGTAGCATGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC407540.15266389201522593No Hit
TACCTGGTTGATCCTGCCAGCTGTAGGCACCATCAATAGATCGGAAGAGC397630.14895162041030152No Hit
CCGATCTTGCCAAGTCGTACTCCTGTAGGCACCATCAATAGATCGGAAGA396730.14861448171762426No Hit
TGTGCGGATGCTGGAGCATCTGTAGGCACCATCAATAGATCGGAAGAGCA393440.14738205249661504No Hit
ACCGGGAATAGCCCAACTTGAAAATCTCCCTGTAGGCACCATCAATAGAT393020.14722472110669899No Hit
TTGCGGTGCGCCGACTTCTTAGAGGCTGTAGGCACCATCAATAGATCGGA391110.14650923788112827No Hit
CGGTGAATCATGATAACTTCCTGTAGGCACCATCAATAGATCGGAAGAGC385400.14437028017536457No Hit
CCCTTCTAAGGCTAAATACTCTGTAGGCACCATCAATAGATCGGAAGAGC382630.14333264219901334No Hit
CCTTCTAAGGCTAAATACTCTGTAGGCACCATCAATAGATCGGAAGAGCA372750.13963160855051152No Hit
GCGGTGAATCATGATAACTTCCTGTAGGCACCATCAATAGATCGGAAGAG372140.1394031034365858No Hit
TGCGGTGCGCCGACTTCTTAGAGGCTGTAGGCACCATCAATAGATCGGAA371240.1390659647439085No Hit
TGAACTGTCTTGGGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACA371200.13905098080201175No Hit
CGGAGGCGTGCAAAGGTTCCCCTGTAGGCACCATCAATAGATCGGAAGAG358190.13417745370008777No Hit
GGGTCGTAAACTGCTTTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC355980.13334959091029133No Hit
GATGGTAGGATAGAGGCCTACCATCTGTAGGCACCATCAATAGATCGGAA351620.13171634124354356No Hit
CGGAGGCGTGCAAAGGTTCCCTCTGTAGGCACCATCAATAGATCGGAAGA348540.13056257771749238No Hit
TTTTCATTCAAATTTCTGCCCTGTAGGCACCATCAATAGATCGGAAGAGC346260.1297084930293766No Hit
TTTTCATTCAAATTTCTGCCCCTGTAGGCACCATCAATAGATCGGAAGAG345430.12939757623501863No Hit
CGCTTCCATGAGTAGGAGGGCCTGTAGGCACCATCAATAGATCGGAAGAG339180.12705633531364854No Hit
ACGGTGGTCATGGAAGTCGAAATCCGCTCTGTAGGCACCATCAATAGATC323600.12122008994485721No Hit
ACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGAGC320430.12003261254953833No Hit
TTTCATTCAAATTTCTGCCCTGTAGGCACCATCAATAGATCGGAAGAGCA319600.11972169575518038No Hit
ACGGAGGCGTGCAAAGGTTCCCTCTGTAGGCACCATCAATAGATCGGAAG317430.11890881690728068No Hit
TCGGAGATAGGGCTCACTGTAGGCACCATCAATAGATCGGAAGAGCACAC307530.11520029128783046No Hit
ATGGATCGTTCATACCTTCCTGTAGGCACCATCAATAGATCGGAAGAGCA300230.11246572189167023No Hit
TGTGCGGATGCTGGAGCATACCCCTGTAGGCACCATCAATAGATCGGAAG300120.11242451605145412No Hit
GCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCGGAAGA296260.11097856565841595No Hit
GCTTAATGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA286930.10748356121099469Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC286530.107333721792027No Hit
GGAGAGTTTGATCCTGGCTCCTGTAGGCACCATCAATAGATCGGAAGAGC284710.10665195243572405No Hit
AACCCTGCACCATCGACCGACCCTGTAGGCACCATCAATAGATCGGAAGA283220.10609380060006941No Hit
GCTTGAAATTGTTGAGAGGCTGTAGGCACCATCAATAGATCGGAAGAGCA274210.1027186676878223No Hit
CGGGAATAGCCCAACTTGAAAATCTCCCTGTAGGCACCATCAATAGATCG272030.10190204285444843No Hit
TTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGCA269560.10097678444232297No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)