FastQCFastQC Report
Wed 30 Aug 2023
SRR11685405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11685405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27059960
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGC17547316.484603081453187No Hit
CCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGAG6377302.356729278239879No Hit
AACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAA5590612.0660082276544385No Hit
TCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGAG4475501.6539196658088187No Hit
GGCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGAG3799521.4041114621012005No Hit
AACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGA3635281.3434166199802218No Hit
GCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGAGC3321981.2276367001281598No Hit
AAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGA2753291.0174774833370042No Hit
AGGAGTGTGTAACAACTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC2521750.9319119466547623No Hit
ACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAG2343070.8658808069191528No Hit
TGTGCGGATGCTGGAGCATACCTGTAGGCACCATCAATAGATCGGAAGAG2015320.7447608939554974No Hit
CCGACTAAGGATACATCCGCCTGTAGGCACCATCAATAGATCGGAAGAGC1939140.7166085980910541No Hit
TACACTGACGCGACCAACGCTGTAGGCACCATCAATAGATCGGAAGAGCA1866420.6897349441758228No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG1516070.5602632080756956No Hit
TGGCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGA1454380.5374656873106982No Hit
TACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGAG1440980.5325137213802238No Hit
TTGTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAG1413580.5223880597014926No Hit
ACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGAA1406250.5196792604275838No Hit
TGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGCAC1336250.4938107816862996No Hit
CGGTGAATCATGATAACTTCTGTAGGCACCATCAATAGATCGGAAGAGCA1313080.4852483152229346No Hit
GCGGTGAATCATGATAACTTCTGTAGGCACCATCAATAGATCGGAAGAGC1291500.477273432776693No Hit
TCGGAGATAGGGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1161030.4290582838999023No Hit
AAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCGG1132300.41844112112508663No Hit
TGCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGAG1127000.4164825077346751No Hit
TCGACTAAGGATACATCCGCCTGTAGGCACCATCAATAGATCGGAAGAGC1070120.3954625210089002No Hit
TGATGGTAGGATAGAGGCCTACCATCTGTAGGCACCATCAATAGATCGGA1036520.38304565121308387No Hit
GCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGAAGAG1011740.3738882097386692No Hit
TTCAGTTATAGTTTATTTGATGCTGTAGGCACCATCAATAGATCGGAAGA988740.36538856672367587No Hit
TAACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGA980590.3623767366987978No Hit
ACCCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAG919570.33982681423032407No Hit
CCCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGA914610.3379938477366559No Hit
TGTGCGGATGCTGGAGCATACCCTGTAGGCACCATCAATAGATCGGAAGA911600.3368815031507807No Hit
TGGAGAATTGTAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT909570.33613131726728346No Hit
GTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGCA875730.3236257555443541No Hit
TGTGCGGATGCTGGAGCATACCCCTGTAGGCACCATCAATAGATCGGAAG772590.2855103998675534No Hit
AAAACTCAAGATGAGTGCTCTCCTGTAGGCACCATCAATAGATCGGAAGA708550.2618444373162414No Hit
TCCCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAG702660.2596677896050105No Hit
CCGATCTTGCCAAGTCGTACTGTAGGCACCATCAATAGATCGGAAGAGCA685030.2531526284591699No Hit
TAACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGG684110.2528126427385702No Hit
ATGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAGAG682290.2521400622912968No Hit
ATCAGTTATAGTTTATTTGATGCTGTAGGCACCATCAATAGATCGGAAGA669660.24747264962697654No Hit
TACCTGGTTGATCCTGCCAGCTGTAGGCACCATCAATAGATCGGAAGAGC658680.24341499396155797No Hit
TGTGCGGATGCTGGAGCATCTGTAGGCACCATCAATAGATCGGAAGAGCA649990.24020360710067568No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCG638620.23600182705369854No Hit
TCAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCG630840.23312673041645296No Hit
AGGATGTTGACACAATGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC624210.230676615929957No Hit
AGGATGTTGACACAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACA615130.22732110468751615No Hit
GGGTCGTAAACTGCTTTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC589410.21781628649857576No Hit
CGGAGGCGTGCAAAGGTTCCCCTGTAGGCACCATCAATAGATCGGAAGAG577610.21345560008218786No Hit
CATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGAAGAGC567490.20971575715559077No Hit
GCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGAG563940.20840385573371134No Hit
GCTGGGTAGCTACGCATGGACTGTAGGCACCATCAATAGATCGGAAGAGC549790.2031747275310089No Hit
GCGAAGAACCTTACCAGGGCTGTAGGCACCATCAATAGATCGGAAGAGCA541130.19997442716101574No Hit
TCCACGAGTCGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC537480.19862557076950593No Hit
TGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGC535100.1977460424923023No Hit
AAATGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAG528460.19529223250884334No Hit
TAAACTCAAGATGAGTGCTCTCCTGTAGGCACCATCAATAGATCGGAAGA510110.18851099558166384No Hit
GCGGTGAATCATGATAACTTCCTGTAGGCACCATCAATAGATCGGAAGAG507510.18755016637127328No Hit
TAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG500810.1850741834060361No Hit
ACGGAGGCGTGCAAAGGTTCCCCTGTAGGCACCATCAATAGATCGGAAGA497010.18366989456008065No Hit
TTTCATTCAAATTTCTGCCCTGTAGGCACCATCAATAGATCGGAAGAGCA495070.18295296814925077No Hit
TCTACACTGACGCGACCAACGCTGTAGGCACCATCAATAGATCGGAAGAG491620.18167802169700178No Hit
ACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGAGC470240.173777049190021No Hit
CGGAGGCGTGCAAAGGTTCCCTCTGTAGGCACCATCAATAGATCGGAAGA452750.16731362500166297No Hit
CGGTGAATCATGATAACTTCCTGTAGGCACCATCAATAGATCGGAAGAGC447780.1654769630110318No Hit
ATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC427740.15807118709709844No Hit
CTGGGTAGCTACGCATGGACTGTAGGCACCATCAATAGATCGGAAGAGCA420160.15527000039911368No Hit
AATGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAGA417570.15431286668568617No Hit
GATGGTAGGATAGAGGCCTACCATCTGTAGGCACCATCAATAGATCGGAA411880.15221012891371605No Hit
TAGCCGAGATATACTAAAGGCCGTCTGTAGGCACCATCAATAGATCGGAA407300.15051759130464346No Hit
GCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCGGAAGA398480.14725816298324165No Hit
AAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG388750.14366244443820317No Hit
ACGGAGGCGTGCAAAGGTTCCCTCTGTAGGCACCATCAATAGATCGGAAG386940.1429935594878928No Hit
GCGAAGAACCTTACCAGGGTCTGTAGGCACCATCAATAGATCGGAAGAGC384620.1421362041924674No Hit
CGTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGC381440.14096103615822048No Hit
CGATGGTAGGATAGAGGCCTACCATCTGTAGGCACCATCAATAGATCGGA374390.13835571079927686No Hit
CCGATCTTGCCAAGTCGTACTCCTGTAGGCACCATCAATAGATCGGAAGA373010.13784573221837726No Hit
CCCGACTAAGGATACATCCGCCTGTAGGCACCATCAATAGATCGGAAGAG359780.13295658973627456No Hit
CGCTTCCATGAGTAGGAGGGCCTGTAGGCACCATCAATAGATCGGAAGAG357720.1321953173618882No Hit
ACCTGGTTGATCCTGCCAGCTGTAGGCACCATCAATAGATCGGAAGAGCA356360.13169272977491467No Hit
TGCGGATGCTGGAGCATACCTGTAGGCACCATCAATAGATCGGAAGAGCA352950.13043256531051783No Hit
GGCGACGGTGAAATACCACTACCTGTAGGCACCATCAATAGATCGGAAGA350310.12945695411227512No Hit
TGCGGTGCGCCGACTTCTTAGAGGGCTGTAGGCACCATCAATAGATCGGA349790.129264788270197No Hit
TCACCCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGA349470.12914653236737972No Hit
TTAGCCGAGATATACTAAAGGCCGTCTGTAGGCACCATCAATAGATCGGA346350.12799353731491103No Hit
CCTTCTAAGGCTAAATACTCTGTAGGCACCATCAATAGATCGGAAGAGCA345100.12753160019453097No Hit
TGTGTGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGC336370.12430543134579652No Hit
TGCGGTGAATCATGATAACTTCTGTAGGCACCATCAATAGATCGGAAGAG334040.12344438055340806No Hit
AACCCTGCACCATCGACCGACCACTGTAGGCACCATCAATAGATCGGAAG332620.12291961998465631No Hit
TTGTCCTAAGATGAGCTCAACGAGAACCTGTAGGCACCATCAATAGATCG327500.1210275255395795No Hit
CCCTTCTAAGGCTAAATACTCTGTAGGCACCATCAATAGATCGGAAGAGC322060.11901717519168543No Hit
ATCCCGAGTAGCATGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC319540.1180859099569992No Hit
GGAGAGTTTGATCCTGGCTCCTGTAGGCACCATCAATAGATCGGAAGAGC319370.11802308650862751No Hit
CAGTTATAGTTTATTTGATGCTGTAGGCACCATCAATAGATCGGAAGAGC311510.1151184258956776No Hit
TTTCATTCAAATTTCTGCCCCTGTAGGCACCATCAATAGATCGGAAGAGC310850.11487452309611691No Hit
TTTTCATTCAAATTTCTGCCCCTGTAGGCACCATCAATAGATCGGAAGAG307400.11359957664386791No Hit
TCGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCG302060.11162618126560424No Hit
TTTTCATTCAAATTTCTGCCCTGTAGGCACCATCAATAGATCGGAAGAGC299060.11051753217669205No Hit
GCTTGAAATTGTTGAGAGGCTGTAGGCACCATCAATAGATCGGAAGAGCA297480.10993364365653165No Hit
ATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT294010.10865130621035657No Hit
TGAACTGTCTTGGGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACA291480.10771634547870729No Hit
AACCCTGCACCATCGACCGACCCTGTAGGCACCATCAATAGATCGGAAGA290790.10746135618825749No Hit
TGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAGAGCA289010.10680355772883626No Hit
GGGTCGTAAACTGCTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA285650.10556187074925462No Hit
TCGGAGATAGGGCTCACTGTAGGCACCATCAATAGATCGGAAGAGCACAC284730.10522188502865489No Hit
GCGACGGTGAAATACCACTACCTGTAGGCACCATCAATAGATCGGAAGAG281540.10404302149744495No Hit
TGCGGTGCGCCGACTTCTTAGAGGCTGTAGGCACCATCAATAGATCGGAA281380.10398389354603628No Hit
TTACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGA274900.10158921151398596No Hit
TGACATGCCAATTGTGTACCAGTCTGTAGGCACCATCAATAGATCGGAAG273140.10093880404849083No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)