FastQCFastQC Report
Wed 30 Aug 2023
SRR11685406_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11685406_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26976556
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGA7322002.714208589117158No Hit
TGTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGC4954331.8365316907021045No Hit
TCGGAGATAGGGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG4109651.5234153685147949No Hit
AACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGA3773181.3986885501618518No Hit
TGGAGAATTGTAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3446681.2776575334523799No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG2725531.0103328238044915No Hit
AACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAA2550400.9454134916258399No Hit
ACCTCAGCCTGCTAAATAGTCAGCATCGCACTGTAGGCACCATCAATAGA2368660.878043883733713No Hit
ACCACGTGCGAACCCTCAGTGACGATCTGTCTGTAGGCACCATCAATAGA2325690.8621152381349199No Hit
AAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCGG1982900.735045644818412No Hit
CCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGAG1872460.6941063937146017No Hit
GCGGTGAATCATGATAACTTCACGAATCGTACTGTAGGCACCATCAATAG1749370.6484778857612513No Hit
TCAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCG1511160.5601752870158815No Hit
TCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGAG1437610.5329108726851567No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATTTCGCTGTAGGCACCATCAATAG1289950.4781744563687077No Hit
AACCCGACTTCTGGAAGGGTCGTATTTATTAGACTGTAGGCACCATCAAT1253560.4646849657161574No Hit
TAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG1177860.43662356306713135No Hit
CGGAGATAGGGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1177740.4365790799982029No Hit
ACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGAA1124700.41691756353183107No Hit
AAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG1100520.40795422514274987No Hit
AAGTGGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1071790.39730423705679846Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAATACCCCATACAGCTGAGGAGTGAAAGGTGCTGTAGGCACCATCAATA948130.3514644345260381No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCG907160.3362771734093855No Hit
ACGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCG869260.322227937472819No Hit
ACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAG849980.3150809910649825No Hit
GGCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGAG844660.3131089083424882No Hit
TCGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCG813870.3016952942399319No Hit
TTGTCCTAAGATGAGCTCAACGAGAACCTGTAGGCACCATCAATAGATCG807780.2994377784918134No Hit
ACCGGGAATAGCCCAACTTGAAAATCTCCCTGTAGGCACCATCAATAGAT794120.2943741224787923No Hit
AGGAGTGTGTAACAACTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC786310.2914790160760328No Hit
TTGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGA782190.28995176404282297No Hit
CAAGCGGTGGATTATGTGGATTAATTCGCTGTAGGCACCATCAATAGATC779080.28879891117309414No Hit
TGGAGAATTGTAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG701520.2600480209556772No Hit
TTCAGTTATAGTTTATTTGATGCTGTAGGCACCATCAATAGATCGGAAGA680430.2522301215915034No Hit
CCGGGAATAGCCCAACTTGAAAATCTCCCTGTAGGCACCATCAATAGATC679160.25175934244534404No Hit
GCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGAGC658920.24425653148608No Hit
TAGCCGAGATATACTAAAGGCCGTCTGTAGGCACCATCAATAGATCGGAA657290.24365230313313532No Hit
TTGTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAG656690.24342988778849312No Hit
ATCAGTTATAGTTTATTTGATGCTGTAGGCACCATCAATAGATCGGAAGA656370.24331126627135058No Hit
TAACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGG651470.2414948742901058No Hit
TGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGCAC607960.22536605488113456No Hit
TACACTGACGCGACCAACGCTGTAGGCACCATCAATAGATCGGAAGAGCA590960.2190642867829385No Hit
CCGACTAAGGATACATCCGCCTGTAGGCACCATCAATAGATCGGAAGAGC587460.21776686393919226No Hit
TACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGAG565110.20948189235126974No Hit
TGTGCGGATGCTGGAGCATACCTGTAGGCACCATCAATAGATCGGAAGAG550570.20409202716610678No Hit
TTAGCCGAGATATACTAAAGGCCGTCTGTAGGCACCATCAATAGATCGGA527150.19541041488023897No Hit
TAGACCTCAGCCTGCTAAATAGTCAGCATCGCACTGTAGGCACCATCAAT518450.19218539238292687No Hit
TAAGTGGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC505700.1874590663092798No Hit
GAGATTCATCCCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGAT496650.18410430152759308No Hit
GCGAAGAACCTTACCAGGGCTGTAGGCACCATCAATAGATCGGAAGAGCA492760.18266230870982939No Hit
TGAGATTCATCCCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGA487260.18062350138394243No Hit
TGCGGTGAATCATGATAACTTCACGAATCGTACTGTAGGCACCATCAATA479900.1778952064896646No Hit
GCGGTGAATCATGATAACTTCACGAATCGTCTGTAGGCACCATCAATAGA479530.17775805036046854No Hit
TCGGGAATAGCCCAACTTGAAAATCTCCCTGTAGGCACCATCAATAGATC469490.17403630026012215No Hit
ACGGTGGTCATGGAAGTCGAAATCCGCTCTGTAGGCACCATCAATAGATC464960.17235706440807344No Hit
ACAAGCGGTGGATTATGTGGATTAATTCGCTGTAGGCACCATCAATAGAT464710.17226439134780586No Hit
TTGTAAACCGCGTCGTGATGGGGATAGATTCTGTAGGCACCATCAATAGA464160.17206051061521715No Hit
TTGCGGTGCGCCGACTTCTTAGAGGGCTGTAGGCACCATCAATAGATCGG463880.17195671678771746No Hit
TCAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATC458690.1700328240565623No Hit
TGTGCGGATGCTGGAGCATACCCCTGTAGGCACCATCAATAGATCGGAAG457110.16944713031567113No Hit
TCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGATC453760.16820531130808544No Hit
TTTGGGCGTAAAGCGTCTGTAGGTGGCTCGCTGTAGGCACCATCAATAGA448320.16618874551666268No Hit
ACCCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAG446290.16543624026728987No Hit
AAATGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAG442590.16406467897532956No Hit
GTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGCA430730.1596682689962351No Hit
AACCCGGCCGATGCCGCCGACTCGACCAAGCTGTAGGCACCATCAATAGA429410.15917895523802222No Hit
ATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT426110.15795567084249004No Hit
ATGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAGAG420710.15595393274071012No Hit
TCGACTAAGGATACATCCGCCTGTAGGCACCATCAATAGATCGGAAGAGC418270.15504944367249845No Hit
TGTGCGGATGCTGGAGCATACCCTGTAGGCACCATCAATAGATCGGAAGA415740.15411159230259044No Hit
TAACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGA403760.14967069925456755No Hit
TTGGGTTGAGTTGGCGGGACAAAAGCTCTGTAGGCACCATCAATAGATCG402880.14934449008242565No Hit
CTGAAAGGACATGCCAATTGTGTACCAGTCTGTAGGCACCATCAATAGAT395080.14645309060207684No Hit
ACCTCAGCCTGCTAAATAGTCAGCATCGCACCTGTAGGCACCATCAATAG383200.14204926677816102No Hit
ATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC382180.14167116069226923No Hit
CGGTGAATCATGATAACTTCTGTAGGCACCATCAATAGATCGGAAGAGCA380760.14114477770994935No Hit
TCGGAGATAGGGCTCACTGTAGGCACCATCAATAGATCGGAAGAGCACAC375930.13935433418557952No Hit
TGCGGTGCGCCGACTTCTTAGAGGGCTGTAGGCACCATCAATAGATCGGA371820.13783078907478036No Hit
TACCTGGTTGATCCTGCCAGCTGTAGGCACCATCAATAGATCGGAAGAGC364540.13513214955978814No Hit
CCAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCG350990.1301092696932848No Hit
AGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGAA346180.12832624001373635No Hit
AGGATGTTGACACAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACA340820.12633932960159924No Hit
CCGATCTTGCCAAGTCGTACTCCTGTAGGCACCATCAATAGATCGGAAGA340440.12619846654999253No Hit
GGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGATC338200.12536811592999492No Hit
ACCTCAGCCTGCTAAATAGTCAGCATCGCCTGTAGGCACCATCAATAGAT338010.12529768440419153No Hit
ATGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGA336720.12481949141321079No Hit
TGGCCCTGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG317380.11765030347091007Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGTGAATCATGATAACTTCTGTAGGCACCATCAATAGATCGGAAGAGC310940.11526304543841698No Hit
ACGCTCTGAATACATTAGCATGGAATAACACGACTGTAGGCACCATCAAT303830.11262742360440674No Hit
AAGCGGTGGATTATGTGGATTAATTCGCTGTAGGCACCATCAATAGATCG299590.11105568850226842No Hit
TGGCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGA298740.1107406000973586No Hit
TAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCG298700.1107257724077158No Hit
AACCCGACTTCTGGAAGGGTCGTATTTATTAGATCTGTAGGCACCATCAA296180.10979162796021849No Hit
CCGATCTTGCCAAGTCGTACTGTAGGCACCATCAATAGATCGGAAGAGCA293020.10862024047843617No Hit
AAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCG292600.10846454973718661No Hit
TCGACTTCTTAGAGGGACTATTGGCGCTGTAGGCACCATCAATAGATCGG291850.10818653055638386No Hit
TCCACGAGTCGGGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG285960.10600315325647945No Hit
TCGCCCATTAAAGTGGTACGTGAGCTGGGCTGTAGGCACCATCAATAGAT278520.10324520298291599No Hit
ATGGCGCTTAAGCGCGTGACCTATACTCGGCCCTGTAGGCACCATCAATA278260.10314882300023769No Hit
CGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCGG277270.10278183768157804No Hit
CGGGAATAGCCCAACTTGAAAATCTCCCTGTAGGCACCATCAATAGATCG275620.10217019548381194No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)