FastQCFastQC Report
Wed 30 Aug 2023
SRR11685407_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11685407_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31513437
Total Bases4.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGC24238887.691601522233199No Hit
CCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGAG9025672.8640703329186215No Hit
AACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAA8565492.7180437348042994No Hit
TCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGAG7815202.4799579937916643No Hit
AACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGA5251261.6663558468725577No Hit
ACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAG5115701.6233392758777787No Hit
AAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGA4969881.577066950837511No Hit
GCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGAGC3998001.2686651728911702No Hit
TTGTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAG3066270.9730039919162101No Hit
GGCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGAG3018470.9578358590337195No Hit
ACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGAA2983830.9468437225682491No Hit
TACACTGACGCGACCAACGCTGTAGGCACCATCAATAGATCGGAAGAGCA2958610.9388407871854789No Hit
TGATGGTAGGATAGAGGCCTACCATCTGTAGGCACCATCAATAGATCGGA2889210.9168184352598543No Hit
CCGACTAAGGATACATCCGCCTGTAGGCACCATCAATAGATCGGAAGAGC2379200.7549795346029695No Hit
TAACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGA2271750.7208829681129354No Hit
TGTGCGGATGCTGGAGCATACCTGTAGGCACCATCAATAGATCGGAAGAG2131860.6764923800599726No Hit
TGCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGAG1968700.6247176402878556No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG1735920.55085073709986No Hit
TGGCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGA1644340.5217901176567951No Hit
AGGAGTGTGTAACAACTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1607540.5101125592870115No Hit
TCGACTAAGGATACATCCGCCTGTAGGCACCATCAATAGATCGGAAGAGC1607280.5100300547985293No Hit
TGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGCAC1575970.5000946104355422No Hit
AAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCGG1569810.4981398887084262No Hit
TAACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGG1525060.48393959694082245No Hit
GCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGAAGAG1449000.45980386081023156No Hit
TACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGAG1293260.4103836722094134No Hit
TTCAGTTATAGTTTATTTGATGCTGTAGGCACCATCAATAGATCGGAAGA1245130.39511082209154147No Hit
TAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG1240380.3936035285519634No Hit
TGTGCGGATGCTGGAGCATCTGTAGGCACCATCAATAGATCGGAAGAGCA1186830.376610777174194No Hit
CGGTGAATCATGATAACTTCTGTAGGCACCATCAATAGATCGGAAGAGCA1169010.3709560464636085No Hit
AAATGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAG1147780.36421923765408387No Hit
TGTGCGGATGCTGGAGCATACCCTGTAGGCACCATCAATAGATCGGAAGA1101470.3495239189555871No Hit
TCCCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAG1093450.3469789728108679No Hit
ATGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAGAG1080070.34273316490359335No Hit
ACCCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAG1070330.3396424198350691No Hit
GCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGAG1034470.32826314692364406No Hit
TCAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCG1029980.3268383578725481No Hit
AAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG1013970.32175798533178085No Hit
CGATGGTAGGATAGAGGCCTACCATCTGTAGGCACCATCAATAGATCGGA913280.28980653554228314No Hit
TGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGC909830.28871176444511587No Hit
TGGAGAATTGTAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT901520.28607479406324354No Hit
CCCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGA891680.2829523164991492No Hit
AGGATGTTGACACAATGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC847070.26879645022534354No Hit
GCGAAGAACCTTACCAGGGCTGTAGGCACCATCAATAGATCGGAAGAGCA838160.26596908487005083No Hit
TCTACACTGACGCGACCAACGCTGTAGGCACCATCAATAGATCGGAAGAG826100.26214214590430107No Hit
ATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC820570.2603873388992765No Hit
GCGGTGAATCATGATAACTTCTGTAGGCACCATCAATAGATCGGAAGAGC796860.2528635641996143No Hit
TGTGCGGATGCTGGAGCATACCCCTGTAGGCACCATCAATAGATCGGAAG766280.24315976705428863No Hit
ACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGAGC702080.22278750489830734No Hit
TACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAA682920.21670755874708303No Hit
GTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGCA682090.21644417903385152No Hit
CCGATCTTGCCAAGTCGTACTGTAGGCACCATCAATAGATCGGAAGAGCA676440.21465129303414288No Hit
ATCAGTTATAGTTTATTTGATGCTGTAGGCACCATCAATAGATCGGAAGA667850.21192547166467435No Hit
TACCTGGTTGATCCTGCCAGCTGTAGGCACCATCAATAGATCGGAAGAGC650210.206327859446115No Hit
CATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGAAGAGC639860.20304354615461334No Hit
CGTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGC590320.1873232678492035No Hit
GCGAAGAACCTTACCAGGGTCTGTAGGCACCATCAATAGATCGGAAGAGC583500.18515911165132512No Hit
TGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAGAGCA578030.1834233441436426No Hit
TCGGAGATAGGGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG562310.17843499584002848No Hit
ACCTGGTTGATCCTGCCAGCTGTAGGCACCATCAATAGATCGGAAGAGCA551410.17497615382289147No Hit
GATGGTAGGATAGAGGCCTACCATCTGTAGGCACCATCAATAGATCGGAA547790.17382743748325516No Hit
AGGATGTTGACACAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACA545650.17314836207805578No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCG545530.1731102830833717No Hit
ATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT540010.17135864932790415No Hit
TGAGATTCATCCCGTGTTGCTACTGTAGGCACCATCAATAGATCGGAAGA516730.16397132435919318No Hit
AACCCTGCACCATCGACCGACCACTGTAGGCACCATCAATAGATCGGAAG513580.16297175074873618No Hit
TCACCCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGA502970.15960493296875233No Hit
GGGTCGTAAACTGCTTTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC499590.15853237461848418No Hit
AATGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAGA498560.1582055299141125No Hit
GCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCGGAAGA474110.15044693474723178No Hit
TACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGA469550.14899993294923686No Hit
TCGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCG463720.1471499284575021No Hit
GCTGGGTAGCTACGCATGGACTGTAGGCACCATCAATAGATCGGAAGAGC457660.14522693922595623No Hit
TCCACGAGTCGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC451150.14316115376434504No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAAT448050.14217744640167304TruSeq Adapter, Index 13 (97% over 40bp)
TCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGAAGAG419630.13315907116066078No Hit
TGCGGTGCGCCGACTTCTTAGAGGGCTGTAGGCACCATCAATAGATCGGA413180.13111232519639163No Hit
AAAACTCAAGATGAGTGCTCTCCTGTAGGCACCATCAATAGATCGGAAGA412640.13094096972031327No Hit
TTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGCA411430.1305570065239155No Hit
TTGCGGTGCGCCGACTTCTTAGAGGGCTGTAGGCACCATCAATAGATCGG408610.12966215014883967No Hit
TTACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGA403140.12792638264115716No Hit
TTGTCCTAAGATGAGCTCAACGAGAACCTGTAGGCACCATCAATAGATCG388060.12314112230919147No Hit
CGCTTCCATGAGTAGGAGGGCCTGTAGGCACCATCAATAGATCGGAAGAG384590.12204000471291024No Hit
CCGATCTTGCCAAGTCGTACTCCTGTAGGCACCATCAATAGATCGGAAGA384460.12199875246866915No Hit
CCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAGA383690.12175441225277966No Hit
GACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGAGCA377760.11987267526547485No Hit
TTTTCATTCAAATTTCTGCCCTGTAGGCACCATCAATAGATCGGAAGAGC372250.1181242147595643No Hit
TAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCG370220.11748004509949199No Hit
TCGGGAATAGCCCAACTTGAAAATCTCCCTGTAGGCACCATCAATAGATC368490.11693107292612989No Hit
ACGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGAG367750.11669625245891142No Hit
TACCCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAA362680.11508741493350914No Hit
ATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCA362330.11497635119901393No Hit
CGGAGGCGTGCAAAGGTTCCCTCTGTAGGCACCATCAATAGATCGGAAGA361200.1146177739990722No Hit
TGAGATTCATCCCGTGTTGCTCTGTAGGCACCATCAATAGATCGGAAGAG356770.11321202444531835No Hit
AACCCTGCACCATCGACCGACCCTGTAGGCACCATCAATAGATCGGAAGA354780.11258054778347408No Hit
CTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAGAGC353890.1122981285729005No Hit
CTGTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAG353080.11204109535878298No Hit
CGGAGGCGTGCAAAGGTTCCCCTGTAGGCACCATCAATAGATCGGAAGAG351470.11153020218010495No Hit
GCTTGAAATTGTTGAGAGGCTGTAGGCACCATCAATAGATCGGAAGAGCA345500.10963577219457211No Hit
CAGTTATAGTTTATTTGATGCTGTAGGCACCATCAATAGATCGGAAGAGC341910.1084965756036068No Hit
TTTCATTCAAATTTCTGCCCTGTAGGCACCATCAATAGATCGGAAGAGCA338160.10730660701972938No Hit
GAGATTCATCCCGTGTTGCTACTGTAGGCACCATCAATAGATCGGAAGAG337750.10717650378789213No Hit
ACCCTGCACCATCGACCGACCACTGTAGGCACCATCAATAGATCGGAAGA337180.10699562856314278No Hit
AGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGAA332880.10563113125363No Hit
TGCGGTGCGCCGACTTCTTAGAGGCTGTAGGCACCATCAATAGATCGGAA329490.10455539965380481No Hit
TGTGCGGATGCTGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA329180.10445702891753762No Hit
TGCGGATGCTGGAGCATACCTGTAGGCACCATCAATAGATCGGAAGAGCA326070.10347014830530864No Hit
TTTTCATTCAAATTTCTGCCCCTGTAGGCACCATCAATAGATCGGAAGAG321000.10186131077990636No Hit
TCCCGACTAAGGATACATCCGCCTGTAGGCACCATCAATAGATCGGAAGA320900.10182957828433631No Hit
CCTTCTAAGGCTAAATACTCTGTAGGCACCATCAATAGATCGGAAGAGCA320890.10182640503477929No Hit
TCGATGGTAGGATAGAGGCCTACCATCTGTAGGCACCATCAATAGATCGG317910.10088077666679139No Hit
AAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCG317870.10086808366856334No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG315330.10006207828108372TruSeq Adapter, Index 13 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)