FastQCFastQC Report
Wed 30 Aug 2023
SRR11685408_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11685408_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23367781
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGA4667991.9976180023255095No Hit
TGGAGAATTGTAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4349201.861195121607824No Hit
TGTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGC4336211.8556361855667853No Hit
AACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGA3222661.379103989377511No Hit
TCGGAGATAGGGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3042771.3021219259115788No Hit
AACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAA2470621.0572762557129407No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG2318760.9922893406096197No Hit
TCAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCG1705810.7299837327301211No Hit
ACCACGTGCGAACCCTCAGTGACGATCTGTCTGTAGGCACCATCAATAGA1538200.6582567681544088No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATTTCGCTGTAGGCACCATCAATAG1359260.5816812473550655No Hit
AAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCGG1342590.574547493405557No Hit
GCGGTGAATCATGATAACTTCACGAATCGTACTGTAGGCACCATCAATAG1250670.5352112808657357No Hit
ACCTCAGCCTGCTAAATAGTCAGCATCGCACTGTAGGCACCATCAATAGA1249040.5345137392377993No Hit
CCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGAG1231840.5271531772742991No Hit
TGGAGAATTGTAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1186550.5077717905692457No Hit
AGGAGTGTGTAACAACTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1170640.5009632707530082No Hit
TCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAGAG1046220.44771901961936394No Hit
AAGTGGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1039780.4449630882795418Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGAA980240.41948356157565836No Hit
GGCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGAG932470.399040884540984No Hit
ACGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCG872790.3735014462862349No Hit
TAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG869330.37202077510055404No Hit
TCGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCG864420.3699195914237642No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCG781020.3342294246937696No Hit
CGGAGATAGGGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT772610.3306304522453373No Hit
AAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG768090.3286961650316733No Hit
CAAGCGGTGGATTATGTGGATTAATTCGCTGTAGGCACCATCAATAGATC743820.31831006974945547No Hit
TAACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGG721800.30888683867757916No Hit
GCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGAGC708580.3032294765172611No Hit
TTAGCCGAGATATACTAAAGGCCGTCTGTAGGCACCATCAATAGATCGGA699450.29932238752151946No Hit
ACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAG660940.282842431636962No Hit
AAATGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAG637660.2728799966072945No Hit
TTGTCCTAAGATGAGCTCAACGAGAACCTGTAGGCACCATCAATAGATCG628970.26916120105713076No Hit
AACCCGACTTCTGGAAGGGTCGTATTTATTAGACTGTAGGCACCATCAAT615180.2632599132968595No Hit
TGTGCGGATGCTGGAGCATACCTGTAGGCACCATCAATAGATCGGAAGAG586880.25114922122900757No Hit
TAATACCCCATACAGCTGAGGAGTGAAAGGTGCTGTAGGCACCATCAATA583400.24965999125034594No Hit
TTCAGTTATAGTTTATTTGATGCTGTAGGCACCATCAATAGATCGGAAGA580610.24846603962952238No Hit
TTGTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAG574460.24583421078792203No Hit
TGCGGTGAATCATGATAACTTCACGAATCGTACTGTAGGCACCATCAATA555130.23756213737196524No Hit
TAAGTGGGAGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC548640.23478480904969112No Hit
TAGCCGAGATATACTAAAGGCCGTCTGTAGGCACCATCAATAGATCGGAA544760.23312440321141317No Hit
TTGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGA541700.23181490788534864No Hit
TCAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATC528380.22611475176012646No Hit
ATCAGTTATAGTTTATTTGATGCTGTAGGCACCATCAATAGATCGGAAGA512740.2194217756491299No Hit
TAACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGA512610.21936614349475458No Hit
TGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGCAC510660.21853166117912523No Hit
ACAAGCGGTGGATTATGTGGATTAATTCGCTGTAGGCACCATCAATAGAT510180.2183262501475857No Hit
AGGATGTTGACACAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACA504730.21599397906031387No Hit
ATGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAGAG502240.2149284093342025No Hit
TGAGATTCATCCCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGA499240.2136445903870804No Hit
TACACTGACGCGACCAACGCTGTAGGCACCATCAATAGATCGGAAGAGCA491620.21038369026139025No Hit
TGGCGACGGTGAAATACCACTACTGTAGGCACCATCAATAGATCGGAAGA485060.20757640616368322No Hit
TTGCGGTGCGCCGACTTCTTAGAGGGCTGTAGGCACCATCAATAGATCGG452630.19369832334529324No Hit
ACCGGGAATAGCCCAACTTGAAAATCTCCCTGTAGGCACCATCAATAGAT432600.185126692175008No Hit
TCGGGAATAGCCCAACTTGAAAATCTCCCTGTAGGCACCATCAATAGATC431780.1847757816627946No Hit
TGGCCCTGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG428800.1835005215086533Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGGAATAGCCCAACTTGAAAATCTCCCTGTAGGCACCATCAATAGATC416030.17803573219040353No Hit
TTGTAAACCGCGTCGTGATGGGGATAGATTCTGTAGGCACCATCAATAGA400800.17151821133551362No Hit
CGGTGAATCATGATAACTTCTGTAGGCACCATCAATAGATCGGAAGAGCA396400.16963527687973454No Hit
CCGACTAAGGATACATCCGCCTGTAGGCACCATCAATAGATCGGAAGAGC394780.1689420146482886No Hit
GCGAAGAACCTTACCAGGGCTGTAGGCACCATCAATAGATCGGAAGAGCA394120.16865957447992172No Hit
TACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGAG359770.15395984753537362No Hit
TGTGCGGATGCTGGAGCATACCCCTGTAGGCACCATCAATAGATCGGAAG355000.15191857540944945No Hit
TGGCGGTAACGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT353600.15131945990079246Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC351120.15025816957117152No Hit
ATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT348290.14904710036438634No Hit
TGTGCGGATGCTGGAGCATACCCTGTAGGCACCATCAATAGATCGGAAGA347900.14888020390126044No Hit
GAGATTCATCCCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGAT346180.14814414770491044No Hit
ACGGTGGTCATGGAAGTCGAAATCCGCTCTGTAGGCACCATCAATAGATC336420.1439674567302732No Hit
GCGGTGAATCATGATAACTTCACGAATCGTCTGTAGGCACCATCAATAGA332780.14240975640776504No Hit
TGCGGTGCGCCGACTTCTTAGAGGGCTGTAGGCACCATCAATAGATCGGA327290.14006036773453157No Hit
GCGGTGAATCATGATAACTTCTGTAGGCACCATCAATAGATCGGAAGAGC326100.13955111955217314No Hit
CCAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCG324840.13901191559438186No Hit
AATGCAGCCCTAATTTGGAGGTCTGTAGGCACCATCAATAGATCGGAAGA317920.1360505732230202No Hit
TCGGAGATAGGGCTCACTGTAGGCACCATCAATAGATCGGAAGAGCACAC314790.1347111221215228No Hit
TCGACTAAGGATACATCCGCCTGTAGGCACCATCAATAGATCGGAAGAGC312570.13376109610065243No Hit
CTGAAAGGACATGCCAATTGTGTACCAGTCTGTAGGCACCATCAATAGAT307950.13178401492208439No Hit
TTTGGGCGTAAAGCGTCTGTAGGTGGCTCGCTGTAGGCACCATCAATAGA298680.12781701437547707No Hit
AAGCGGTGGATTATGTGGATTAATTCGCTGTAGGCACCATCAATAGATCG292840.12531784682507935No Hit
TCGCCCATTAAAGTGGTACGTGAGCTGGGCTGTAGGCACCATCAATAGAT287530.12304548728867325No Hit
TTGGAGAATTGTAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG286570.12263466522559416No Hit
TTGTAAACCGCGTCGTGATGGGGATAGATTACTGTAGGCACCATCAATAG277720.11884739933158395No Hit
ACCCGACTAAGGATACATCCGCCCTGTAGGCACCATCAATAGATCGGAAG277550.11877464959124703No Hit
GCTTAATGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA271390.1161385413531563Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA268000.11468782594290831Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGGTTGAGTTGGCGGGACAAAAGCTCTGTAGGCACCATCAATAGATCG267010.11426416569035802No Hit
TGATGGTAGGATAGAGGCCTACCATCTGTAGGCACCATCAATAGATCGGA265210.11349387432208476No Hit
TTACCCAATCCCGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT260680.11155530771193038No Hit
TTGGGTTGAGTTGGCGGGACAAAAGCTGGGTCTGTAGGCACCATCAATAG258870.1107807369471667No Hit
TACCTGGTTGATCCTGCCAGCTGTAGGCACCATCAATAGATCGGAAGAGC257190.11006179833677832No Hit
TCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGATC256500.10976651997894023No Hit
TCAAGCGGTGGATTATGTGGATTAATTCGCTGTAGGCACCATCAATAGAT252980.10826017241431696No Hit
AGGCCCTGGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG246160.10534162400785936Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGAA242190.10364270360116777No Hit
TAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCG241530.10336026343280091No Hit
AACCCGACTTCTGGAAGGGTCGTATTTATTAGATCTGTAGGCACCATCAA239250.1023845610329881No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATTTCCTGTAGGCACCATCAATAGA236420.10117349182620292No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)