FastQCFastQC Report
Wed 30 Aug 2023
SRR11685409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11685409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16660476
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCCTGTAGGCACCA3546652.1287807143085224No Hit
CAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCCTGTAGGCACCAT3272611.9642956179643365No Hit
ACCACGTGCGAACCCTCAGTGACGATCTGTCTGTAGGCACCATCAATAGA3080581.8490348054881505No Hit
AGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCCTGTAGGCACCATC2501221.5012896390235189No Hit
GTGAATCATCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATC2322071.393759698102263No Hit
TCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCAT2120831.2729708322859443No Hit
TTGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGA2093171.256368665577142No Hit
CAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCATC1967421.180890630015613No Hit
TAATACCCCATACAGCTGAGGAGTGAAAGGTGCTGTAGGCACCATCAATA1876001.126018248218118No Hit
GCGGTGAATCATGATAACTTCACGAATCGTACTGTAGGCACCATCAATAG1843451.1064809912994082No Hit
AGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCATCA1624780.9752302395201674No Hit
TTGGGTTGAGTTGGCGGGACAAAAGCTGGGTCTGTAGGCACCATCAATAG1507900.9050761814968552No Hit
ACGAGACCTCAGCCTGCTAAATAGTCAGCATCGCACTGTAGGCACCATCA1364140.8187881306632536No Hit
CCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCCTGTAGGCACCA1241550.7452067996136484No Hit
ACGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCG1166460.7001360585375832No Hit
ATGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGA1142430.6857127011257061No Hit
AACCCGACTTCTGGAAGGGTCGTATTTATTAGACTGTAGGCACCATCAAT1085210.6513679441091599No Hit
TGAATCATCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATCA902910.5419473009054483No Hit
TTGAATCATCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATC863610.5183585390957617No Hit
GAGATTCATCCCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGAT843470.5062700489469808No Hit
CCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCAT804040.4826032581542088No Hit
ATGGGTTGAGTTGGCGGGACAAAAGCTGGGTCTGTAGGCACCATCAATAG794720.47700918028992684No Hit
GCGGTGAATCATGATAACTTCACGAATCGTCTGTAGGCACCATCAATAGA780840.46867808578818515No Hit
TTACCCAATGATGTCACCCGACTAAGGATACATCCGCCCTGTAGGCACCA755190.4532823672024737No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATTTCGCTGTAGGCACCATCAATAG753260.4521239369151278No Hit
TCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGCTGTAGGCACCATC719910.4321065016389688No Hit
AGGTAACCCGGCCGATGCCGCCGACTCGACCAAGCTGTAGGCACCATCAA676770.40621288371352654No Hit
CAAGCGGTGGATTATGTGGATTAATTCGCTGTAGGCACCATCAATAGATC674820.40504244896724434No Hit
TGAGATTCATCCCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGA671900.4032897979625552No Hit
CAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGCTGTAGGCACCATCA661740.3971915328229518No Hit
AAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCGG620700.3725583830858134No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG614920.3690890944532437No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA609740.36597993958876085Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCG592980.3559202030002024No Hit
ACCTCAGCCTGCTAAATAGTCAGCATCGCACTGTAGGCACCATCAATAGA580520.3484414250829328No Hit
ACGCTCTGAATACATTAGCATGGAATAACACGACTGTAGGCACCATCAAT573720.3443599090446155No Hit
AAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGA510110.3061797273979447No Hit
TGGTCAGAATGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCA504840.3030165524682488No Hit
TCCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCCTGTAGGCACC493320.2961019841209819No Hit
ATCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGAT445660.26749535847595235No Hit
TGCGGTGAATCATGATAACTTCACGAATCGTACTGTAGGCACCATCAATA422810.25378026414131266No Hit
TCGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCG414690.24890645381320436No Hit
TTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGATCGGAAGAGC395760.23754423343006525No Hit
TGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGAT395210.237214110809319No Hit
ATGGCTCATTAAATCAGTTATAGTTTATTTGATGCTGTAGGCACCATCAA380110.22815074431246746No Hit
ACGAGACCTCAGCCTGCTAAATAGTCAGCATCGCCTGTAGGCACCATCAA365930.21963958292668231No Hit
TCCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCA357780.2147477659101697No Hit
AACCCGACTTCTGGAAGGGTCGTATTTATTAGATCTGTAGGCACCATCAA355760.2135353155576107No Hit
GGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCCTGTAGGCACCATCA343720.2063086312780019No Hit
GGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGATC343490.2061705800002353No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT332740.1997181833220131TruSeq Adapter, Index 4 (100% over 43bp)
GCGCGCCGTTGACGGTCCTTGAAAATCCGGGGGAGCACTGTAGGCACCAT300080.180114901879154No Hit
GCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCCTGTAGGCACCA294440.17672964445913789No Hit
AACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGA292970.17584731672732518No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT290290.17423871922987075Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTCAGAATGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACC266600.16001943761990955No Hit
ACGAGACCTCAGCCTGCTAAATAGTCAGCATCGCACCTGTAGGCACCATC265780.15952725480352423No Hit
TGGGTTGAGTTGGCGGGACAAAAGCTGGGTCTGTAGGCACCATCAATAGA263170.15796067291234658No Hit
ACGTGCGAACCCTCAGTGACGATCTGTCTGTAGGCACCATCAATAGATCG261620.15703032734478894No Hit
TCGACCAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG259180.15556578335456922Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGACTCGACCAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA256990.15425129510105234No Hit
CCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGCTGTAGGCACCATC256360.1538731546445612No Hit
TAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG255080.15310486927264264TruSeq Adapter, Index 4 (100% over 35bp)
TCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGATC254770.1529188001591311No Hit
CCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA253370.1520784880335952No Hit
TACCCAATGATGTCACCCGACTAAGGATACATCCGCCCTGTAGGCACCAT247410.14850115927059948No Hit
GGGTTGAGTTGGCGGGACAAAAGCTGGGTCTGTAGGCACCATCAATAGAT242900.14579415378048022No Hit
ACAAGCGGTGGATTATGTGGATTAATTCGCTGTAGGCACCATCAATAGAT241590.14500786172015734No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC240460.1443296097902605Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCGG240390.1442875941839837No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC238430.14311115720823342Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATTCATCCCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGATC234260.14060822752003005No Hit
TCAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCG233940.1404161561770504No Hit
TTTTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGATCGGAAGA233510.14015806030992153No Hit
TTTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGATCGGAAGAG225170.13515220093351474No Hit
ACGAATCGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA223330.13404779071138184Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCGGTGGATTATGTGGATTAATTCGCTGTAGGCACCATCAATAGATCG221540.1329733916365895No Hit
GGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCATCAA219130.1315268543347741No Hit
CTGAAAGGACATGCCAATTGTGTACCAGTCTGTAGGCACCATCAATAGAT218600.13120873617296408No Hit
ACCTCAGCCTGCTAAATAGTCAGCATCGCCTGTAGGCACCATCAATAGAT217420.13050047309572668No Hit
ACCCAATGATGTCACCCGACTAAGGATACATCCGCCCTGTAGGCACCATC217140.1303324106706195No Hit
AAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA213180.1279555278012465Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGTGAATCATGATAACTTCACGAATCGTATCTGTAGGCACCATCAATA210000.12604681883038638No Hit
TCAGTGACGATCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG208800.12532655129421272No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATTTCCTGTAGGCACCATCAATAGA206730.1240840897943132No Hit
AAATGGCTCATTAAATCAGTTATAGTTTATTTGATGCTGTAGGCACCATC206550.12397604966388716No Hit
TTTAGTCTTCAACGAGGAATGCCTAGTAAGCGCGACTGTAGGCACCATCA206390.12388001399239734No Hit
TTGGGTTGAGTTGGCGGGACAAAAGCTCTGTAGGCACCATCAATAGATCG206300.12382599392718431No Hit
TTGCGGTGCGCCGACTTCTTAGAGGGACTATTGGCGCTGTAGGCACCATC206150.12373596048516262No Hit
ACGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA205250.12319575983303238Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGAT202970.12182725151430246No Hit
ATGCCGCCGACTCGACCAAGGCCTGTAGGCACCATCAATAGATCGGAAGA200280.12021265178737989No Hit
TGCGGTGCGCCGACTTCTTAGAGGGACTATTGGCGCTGTAGGCACCATCA196580.11799182688417785No Hit
TCAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATC194470.11672535646640589No Hit
TGGTCAGAATGGGTTGAGTTGGCGGGACAAAAGCTGGGTCTGTAGGCACC194150.11653328512342624No Hit
ATCCCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGATCGGAAGA192670.11564495516214542No Hit
AACTCTCAACAACGGATATCTTGGCTCTCGGATCTGTAGGCACCATCAAT190860.11455855162841685No Hit
TACGCTCTGAATACATTAGCATGGAATAACACGACTGTAGGCACCATCAA189730.11388029969852001No Hit
AAAAGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA189120.11351416370096509Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAA187200.11236173564308725No Hit
GTAACCCGGCCGATGCCGCCGACTCGACCAAGGCCTGTAGGCACCATCAA184820.11093320502967621No Hit
TTTGGGCGTAAAGCGTCTGTAGGTGGCTCGCTGTAGGCACCATCAATAGA182030.10925858300807252No Hit
TAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCCTGTAGGCACCATCAA181370.10886243586317702No Hit
GCCGACTCGACCAAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC180150.10813016386806715No Hit
ATGATAACTTCACGAATCGTACTGTAGGCACCATCAATAGATCGGAAGAG177100.10629948388029249No Hit
GCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCAT176540.10596335903007813No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATTTCGGCTGTAGGCACCATCAATA173120.10391059655198327No Hit
AACCCGGCCGATGCCGCCGACTCGACCAAGCTGTAGGCACCATCAATAGA170610.10240403695548675No Hit
ATTAAGGCGTACGGTGGAGACCTAGGCACTCAGAGCTGTAGGCACCATCA169990.10203189872846369No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)