FastQCFastQC Report
Wed 30 Aug 2023
SRR11685410_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11685410_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25338161
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGA5095792.011112803332491No Hit
ACCACGTGCGAACCCTCAGTGACGATCTGTCTGTAGGCACCATCAATAGA3498211.3806092715252698No Hit
GTGAATCATCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATC3191921.2597283599232005No Hit
ATGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGA3051501.2043099734033578No Hit
ACGAGACCTCAGCCTGCTAAATAGTCAGCATCGCACTGTAGGCACCATCA2962991.1693784722577145No Hit
AGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCATCA2888631.1400314332204298No Hit
TCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCAT2559891.010290367955275No Hit
TTGAATCATCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATC2276510.898451154367517No Hit
CAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCATC2051480.8096404470711194No Hit
TGAATCATCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATCA1961810.7742511384310803No Hit
GCGGTGAATCATGATAACTTCACGAATCGTACTGTAGGCACCATCAATAG1911280.7543088861105587No Hit
AAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGA1736380.6852825664814427No Hit
AGGTAACCCGGCCGATGCCGCCGACTCGACCAAGCTGTAGGCACCATCAA1722200.6796862645240908No Hit
TTGGGTTGAGTTGGCGGGACAAAAGCTGGGTCTGTAGGCACCATCAATAG1702170.6717811920131063No Hit
GGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGATC1641550.6478568038146099No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1624350.6410686237252972Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGAT1581980.6243468103308681No Hit
AACCCGACTTCTGGAAGGGTCGTATTTATTAGACTGTAGGCACCATCAAT1519690.5997633372050954No Hit
TCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGCTGTAGGCACCATC1519550.5997080845764615No Hit
AAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCGG1432430.5653251631008265No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATTTCGCTGTAGGCACCATCAATAG1421050.5608339137161532No Hit
AGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCCTGTAGGCACCATC1408320.5558098711267957No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG1398280.5518474683304759No Hit
CAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGCTGTAGGCACCATCA1188390.4690119381592058No Hit
ACCTCAGCCTGCTAAATAGTCAGCATCGCACTGTAGGCACCATCAATAGA1150040.453876664529837No Hit
TCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCCTGTAGGCACCA1148740.4533636044068076No Hit
TTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGATCGGAAGAGC1144180.4515639473598735No Hit
ATCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGAT1109760.43797969394858605No Hit
ATGGGTTGAGTTGGCGGGACAAAAGCTGGGTCTGTAGGCACCATCAATAG1080560.4264555742620785No Hit
TCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGATC1056720.41704684092898453No Hit
TTGGGTTGAGTTGGCGGGACAAAAGCTCTGTAGGCACCATCAATAGATCG997310.3935999933065387No Hit
GCGGTGAATCATGATAACTTCACGAATCGTCTGTAGGCACCATCAATAGA979880.3867210410416131No Hit
ACGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCG928600.3664827924962668No Hit
TCGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCG917120.3619520769482837No Hit
CAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCCTGTAGGCACCAT885620.34952023550564704No Hit
ACGCTCTGAATACATTAGCATGGAATAACACGACTGTAGGCACCATCAAT805950.31807754319660375No Hit
TTTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGATCGGAAGAG749970.29598438497568946No Hit
TTTTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGATCGGAAGA715800.28249879697267694No Hit
CAAGCGGTGGATTATGTGGATTAATTCGCTGTAGGCACCATCAATAGATC696570.27490945376817205No Hit
CCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCAT674910.26636108279523524No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCG651540.2571378404296981No Hit
ACGAGACCTCAGCCTGCTAAATAGTCAGCATCGCCTGTAGGCACCATCAA599940.23677330016175996No Hit
TTACCCAATGATGTCACCCGACTAAGGATACATCCGCCCTGTAGGCACCA583860.23042714110151877No Hit
GGGTTGAGTTGGCGGGACAAAAGCTGGGTCTGTAGGCACCATCAATAGAT573100.22618058192936732No Hit
ATGGGTTGAGTTGGCGGGACAAAAGCTCTGTAGGCACCATCAATAGATCG559440.2207895040212271No Hit
AACCCGGCCGATGCCGCCGACTCGACCAAGCTGTAGGCACCATCAATAGA556140.21948712063199852No Hit
TGGGTTGAGTTGGCGGGACAAAAGCTGGGTCTGTAGGCACCATCAATAGA547780.21618774937928603No Hit
ACGAGACCTCAGCCTGCTAAATAGTCAGCATCGCACCTGTAGGCACCATC527440.20816033176204066No Hit
CTGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGA527150.20804587988844178No Hit
CGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCGG512300.20218515463691308No Hit
TGAGATTCATCCCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGA505540.19951724199715995No Hit
GAGATTCATCCCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGAT491140.19383411448052604No Hit
GCGCGCCGTTGACGGTCCTTGAAAATCCGGGGGAGCACTGTAGGCACCAT489890.19334078743915156No Hit
ACGTGCGAACCCTCAGTGACGATCTGTCTGTAGGCACCATCAATAGATCG482810.19054658307680655No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC473580.18690385620329747Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAAAGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA453600.17901851677396793Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA440730.17393922155597638Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA436220.17215929759069729Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCATCAATAG426300.1682442541903495No Hit
ATGGCTCATTAAATCAGTTATAGTTTATTTGATGCTGTAGGCACCATCAA425610.16797193766351076No Hit
CCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGCTGTAGGCACCATC421970.16653536931902832No Hit
GGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCATCAA401780.15856715094674786No Hit
TCAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCG400540.1580777705217044No Hit
GCTACGATCCACTGAGATTCATCCCGTGTTGCTCTGTAGGCACCATCAAT396450.15646360444232713No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATTTCCTGTAGGCACCATCAATAGA394710.15577689320073387No Hit
TGCGGTGCGCCGACTTCTTAGAGGGACTATTGGCGCTGTAGGCACCATCA377830.14911500483401302No Hit
AACCCGACTTCTGGAAGGGTCGTATTTATTAGATCTGTAGGCACCATCAA374320.1477297425018335No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATAT374140.14765870340787557TruSeq Adapter, Index 25 (97% over 43bp)
TGGTCAGAATGGGTTGAGTTGGCGGGACAAAAGCTCTGTAGGCACCATCA358520.14149408869886018No Hit
TGCGGTGAATCATGATAACTTCACGAATCGTACTGTAGGCACCATCAATA350270.13823813022578868No Hit
TGTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGC337280.13311147561182518No Hit
TACCCAATGATGTCACCCGACTAAGGATACATCCGCCCTGTAGGCACCAT336750.1329023049462824No Hit
TAGACCTCAGCCTGCTAAATAGTCAGCATCGCACTGTAGGCACCATCAAT324730.1281584721164255No Hit
TCAGCCTGCTAAATAGTCAGCATCGCACTGTAGGCACCATCAATAGATCG324610.12811111272045356No Hit
TTGCGGTGCGCCGACTTCTTAGAGGGACTATTGGCGCTGTAGGCACCATC324380.12802034054484066No Hit
GGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGAT321670.12695080751914078No Hit
TGGTCAGAATGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCA308720.12183993937050128No Hit
GGGTTGAGTTGGCGGGACAAAAGCTCTGTAGGCACCATCAATAGATCGGA308130.12160708900697252No Hit
TAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC307280.12127162661883788TruSeq Adapter, Index 5 (100% over 35bp)
TGGGTTGAGTTGGCGGGACAAAAGCTCTGTAGGCACCATCAATAGATCGG304490.12017052066249008No Hit
TTGGGTTGAGTTGGCGGGACAAAAGCTGGCTGTAGGCACCATCAATAGAT304100.12001660262558123No Hit
TGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCA303770.11988636428665836No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC302000.11918781319607212Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGA299480.11819326588066117No Hit
CCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCCTGTAGGCACCA292140.1152964494937103No Hit
ATTAAGGCGTACGGTGGAGACCTAGGCACTCAGAGCTGTAGGCACCATCA291730.11513463822413947No Hit
GAGACCTCAGCCTGCTAAATAGTCAGCATCGCACTGTAGGCACCATCAAT288100.113702016495988No Hit
TCAGTGACGATCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG285740.112770615041873No Hit
CTCAGTGACGATCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC281770.11120380835846769No Hit
AAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA280570.1107302143987482Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGTCCTAAGATGAGCTCAACGAGAACCTGTAGGCACCATCAATAGATCG280380.11065522868845927No Hit
CGCGCCGTTGACGGTCCTTGAAAATCCGGGGGAGCACTGTAGGCACCATC279200.11018952796140177No Hit
CGCTCTGAATACATTAGCATGGAATAACACGACTGTAGGCACCATCAATA275840.1088634648741872No Hit
GGTAACCCGGCCGATGCCGCCGACTCGACCAAGCTGTAGGCACCATCAAT271440.10712695368854906No Hit
TACGCTCTGAATACATTAGCATGGAATAACACGACTGTAGGCACCATCAA268800.10608504697716618No Hit
ATTAAGGCGTACGGTGGAGACCTAGGCACTCAGACTGTAGGCACCATCAA264420.10435642902419003No Hit
CCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA264350.10432880270987306No Hit
TAATACCCCATACAGCTGAGGACTGTAGGCACCATCAATAGATCGGAAGA261680.10327505614949718No Hit
TTGGTCAGAATGGGTTGAGTTGGCGGGACAAAAGCTCTGTAGGCACCATC259480.10240680055667814No Hit
GCTACGATCCACTGAGATTCATCCCGTGTTGCTACTGTAGGCACCATCAA258980.10220946974012833No Hit
TAACCCGGCCGATGCCGCCGACTCGACCAAGCTGTAGGCACCATCAATAG255970.10102153822449862No Hit
TGGCCGGGACTAAGTGACCTGCCGAAGCTGTGATACTGTAGGCACCATCA253770.10015328263167954No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)