FastQCFastQC Report
Wed 30 Aug 2023
SRR11685411_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11685411_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25852634
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGA6967682.6951528420663053No Hit
GTGAATCATCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATC4036251.5612529075373904No Hit
AACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGA3620821.400561350924629No Hit
TTGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGA3594161.390249055473419No Hit
ACGAGACCTCAGCCTGCTAAATAGTCAGCATCGCACTGTAGGCACCATCA3449781.3344017479998365No Hit
ACCACGTGCGAACCCTCAGTGACGATCTGTCTGTAGGCACCATCAATAGA3242621.2542706480121135No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG3120451.2070143413626635No Hit
GCGGTGAATCATGATAACTTCACGAATCGTACTGTAGGCACCATCAATAG2265340.8762511394390219No Hit
TTGGGTTGAGTTGGCGGGACAAAAGCTGGGTCTGTAGGCACCATCAATAG2196900.8497780148823522No Hit
AACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAA2169020.8389938139378759No Hit
ATGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGA1865480.7215821799821248No Hit
AAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCGG1828390.7072354793712703No Hit
AGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCATCA1661420.6426501841166358No Hit
TGAATCATCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATCA1648850.6377880103048688No Hit
TCGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCG1602250.6197627676932261No Hit
TTGAATCATCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATC1558920.6030023865266495No Hit
AACCCGACTTCTGGAAGGGTCGTATTTATTAGACTGTAGGCACCATCAAT1529600.5916611823770066No Hit
ACGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCG1528750.5913323957628457No Hit
ACCTCAGCCTGCTAAATAGTCAGCATCGCACTGTAGGCACCATCAATAGA1448770.5603955094092153No Hit
AGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCCTGTAGGCACCATC1350620.5224303256681698No Hit
TCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCAT1242630.4806589533584856No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATTTCGCTGTAGGCACCATCAATAG1199480.46396819759255475No Hit
TTACCCAATGATGTCACCCGACTAAGGATACATCCGCCCTGTAGGCACCA1180620.4566730028359973No Hit
TCAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCG1139010.4405779310533696No Hit
ATGGGTTGAGTTGGCGGGACAAAAGCTGGGTCTGTAGGCACCATCAATAG1129570.4369264655972772No Hit
ACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGAA1048320.4054983333613124No Hit
GAGATTCATCCCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGAT1048140.4054287079606666No Hit
TGAGATTCATCCCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGA1005830.3890628707310829No Hit
CAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCATC1000630.3870514702679812No Hit
TTGGGTTGAGTTGGCGGGACAAAAGCTCTGTAGGCACCATCAATAGATCG954090.36904943612322055No Hit
GCGGTGAATCATGATAACTTCACGAATCGTCTGTAGGCACCATCAATAGA936160.36211397260333317No Hit
AGGTAACCCGGCCGATGCCGCCGACTCGACCAAGCTGTAGGCACCATCAA873990.3380661328358263No Hit
AAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG845240.32694540912156184No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCG844200.3265431290289415No Hit
TAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG838060.32416812925135596No Hit
CAAGCGGTGGATTATGTGGATTAATTCGCTGTAGGCACCATCAATAGATC812570.31430839890434376No Hit
ACGAGACCTCAGCCTGCTAAATAGTCAGCATCGCCTGTAGGCACCATCAA784350.3033926833142031No Hit
TGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGAT763530.2953393453061688No Hit
CGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCGG757870.2931500132636388No Hit
ATCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGAT740620.28647757903508014No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTAT734370.28406003040154437TruSeq Adapter, Index 20 (97% over 43bp)
TCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGCTGTAGGCACCATC716390.27710522649258873No Hit
TCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCCTGTAGGCACCA712090.2754419530327161No Hit
TCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGATC690340.2670288837880117No Hit
TTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGATCGGAAGAGC648000.2506514423249871No Hit
TGCGGTGAATCATGATAACTTCACGAATCGTACTGTAGGCACCATCAATA621160.2402695214731311No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG595210.2302318595466907TruSeq Adapter, Index 20 (97% over 43bp)
ACGCTCTGAATACATTAGCATGGAATAACACGACTGTAGGCACCATCAAT585750.22657265793497097No Hit
CAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCCTGTAGGCACCAT585450.22645661560056124No Hit
GGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGATC572560.22147066329875711No Hit
CAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGCTGTAGGCACCATCA555430.21484464600396233No Hit
TAACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGG552490.21370743112674706No Hit
TTGTCCTAAGATGAGCTCAACGAGAACCTGTAGGCACCATCAATAGATCG541990.20964594942240702No Hit
TAATACCCCATACAGCTGAGGAGTGAAAGGTGCTGTAGGCACCATCAATA540520.2090773419837994No Hit
AACCCGACTTCTGGAAGGGTCGTATTTATTAGATCTGTAGGCACCATCAA533170.20623430479076135No Hit
ACGAGACCTCAGCCTGCTAAATAGTCAGCATCGCACCTGTAGGCACCATC533140.2062227005573204No Hit
ACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAG529360.20476056714375795No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGC469030.18142445369396404TruSeq Adapter, Index 20 (97% over 42bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC447550.17311582255022834Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGTTGAGTTGGCGGGACAAAAGCTGGGTCTGTAGGCACCATCAATAGA442450.17114310286526319No Hit
GGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCGGA441150.17064025274948774No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA440860.17052807849289167Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGGCTCATTAAATCAGTTATAGTTTATTTGATGCTGTAGGCACCATCAA428270.16565816852549725No Hit
TTTTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGATCGGAAGA416960.16128337251825095No Hit
TACCCAATGATGTCACCCGACTAAGGATACATCCGCCCTGTAGGCACCAT415570.16074570970215257No Hit
TGTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGC411800.15928744436640382No Hit
ATGGGTTGAGTTGGCGGGACAAAAGCTCTGTAGGCACCATCAATAGATCG407730.15771313669624534No Hit
ACCCAATGATGTCACCCGACTAAGGATACATCCGCCCTGTAGGCACCATC406910.15739595431552544No Hit
AGATTCATCCCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGATC404990.15665328337530326No Hit
ACCTCAGCCTGCTAAATAGTCAGCATCGCCTGTAGGCACCATCAATAGAT398360.15408874778484852No Hit
TTTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGATCGGAAGAG391890.15158610143941234No Hit
CCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA381030.1473853689337806No Hit
GCTACGATCCACTGAGATTCATCCCGTGTTGCTCTGTAGGCACCATCAAT365860.14151749489046261No Hit
TGGTCAGAATGGGTTGAGTTGGCGGGACAAAAGCTCTGTAGGCACCATCA364350.14093341514060037No Hit
TCAGTGACGATCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG359040.1388794658215484No Hit
TTGTCCTAAGATGAGCTCAACGAGAACTGTAGGCACCATCAATAGATCGG351190.1358430247378275No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG349330.13512356226448724Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGTGCGAACCCTCAGTGACGATCTGTCTGTAGGCACCATCAATAGATCG348740.13489534567348146No Hit
GGGTTGAGTTGGCGGGACAAAAGCTGGGTCTGTAGGCACCATCAATAGAT342910.13264025630811932No Hit
TAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCCTGTAGGCACCATCAA342320.13241203971711354No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATTTCCTGTAGGCACCATCAATAGA340430.13168097301033233No Hit
TTGCGGTGCGCCGACTTCTTAGAGGGACTATTGGCGCTGTAGGCACCATC337830.13067527277878146No Hit
TGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCGG327990.12686908421014276No Hit
TGCGGTGCGCCGACTTCTTAGAGGGACTATTGGCGCTGTAGGCACCATCA324820.12564290354321342No Hit
CCAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCG313890.12141509449288611No Hit
ACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCT303040.11721823006506803TruSeq Adapter, Index 20 (100% over 40bp)
TAACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGA298120.11531513578074869No Hit
GGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCATCAA297700.11515267651257509No Hit
TAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT297040.11489738337687369TruSeq Adapter, Index 18 (100% over 35bp)
CCAGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCAT296710.11476973680902303No Hit
GGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGAT289870.11212397158448148No Hit
ATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT287750.11130393908798616No Hit
AAGCGGTGGATTATGTGGATTAATTCGCTGTAGGCACCATCAATAGATCG285430.1104065450352177No Hit
TTTAGTCTTCAACGAGGAATGCCTAGTAAGCGCGACTGTAGGCACCATCA278940.10789616253415416No Hit
TCAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATC278120.10757898015343427No Hit
GGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCCTGTAGGCACCATCA277900.10749388244153382No Hit
ACAAGCGGTGGATTATGTGGATTAATTCGCTGTAGGCACCATCAATAGAT276140.10681310074633014No Hit
ATCCCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGATCGGAAGA269870.10438781595716708No Hit
AACCCGGCCGATGCCGCCGACTCGACCAAGCTGTAGGCACCATCAATAGA269750.1043413990234032No Hit
GAGACCTCAGCCTGCTAAATAGTCAGCATCGCACTGTAGGCACCATCAAT265330.1026317086297667No Hit
ACGAATCGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA263470.10191224615642645Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCATCAATAG263390.1018813015339172No Hit
ACCGGGAATAGCCCAACTTGAAAATCTCCCTGTAGGCACCATCAATAGAT259290.10029538963031776No Hit
AGCGGAGGAAAAGAAACTAACAAGGATTCCCCCTGTAGGCACCATCAATA258990.10017934729590802No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)