FastQCFastQC Report
Wed 30 Aug 2023
SRR11685412_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11685412_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25342302
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGA10312374.06923175329534No Hit
AACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAA4815051.9000049798159617No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG4661061.8392409655602717No Hit
AACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGA4427441.7470551807014216No Hit
GCGGTGAATCATGATAACTTCACGAATCGTACTGTAGGCACCATCAATAG2551281.0067278023914323No Hit
ACGAGACCTCAGCCTGCTAAATAGTCAGCATCGCACTGTAGGCACCATCA2325000.9174383605719797No Hit
AAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCGG2177860.8593773367549641No Hit
ACCACGTGCGAACCCTCAGTGACGATCTGTCTGTAGGCACCATCAATAGA2049260.8086321439938645No Hit
GTGAATCATCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATC1868580.7373363319559525No Hit
TTGGGTTGAGTTGGCGGGACAAAAGCTGGGTCTGTAGGCACCATCAATAG1576630.6221336956682151No Hit
TTGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGA1568510.618929566856239No Hit
TTGAATCATCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATC1481950.5847732380428582No Hit
TTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGATCGGAAGAGC1414940.5583312834011686No Hit
AACCCGACTTCTGGAAGGGTCGTATTTATTAGACTGTAGGCACCATCAAT1311250.5174155055053798No Hit
ACGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCG1268760.5006490728427118No Hit
TCAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCG1252840.4943670863049458No Hit
GCGGTGAATCATGATAACTTCACGAATCGTCTGTAGGCACCATCAATAGA1215090.47947104410641145No Hit
TAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG1185300.46771599517676016No Hit
TCGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCG1152010.4545798562419468No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATCG1138710.4493317142223307No Hit
AAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGG1097320.4329993384184278No Hit
TGAATCATCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATCA1073040.42341851975404604No Hit
ATCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGAT1062080.41909373505216696No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1060830.41860048862175187Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGAA1026340.40499083311374No Hit
ACCTCAGCCTGCTAAATAGTCAGCATCGCACTGTAGGCACCATCAATAGA1017610.4015460000437214No Hit
ATGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGA999700.3944787651887346No Hit
ATGGGTTGAGTTGGCGGGACAAAAGCTGGGTCTGTAGGCACCATCAATAG928570.36641107031239706No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATTTCGCTGTAGGCACCATCAATAG822030.3243706905552621No Hit
TTTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGATCGGAAGAG820400.3237274972100009No Hit
ACGCTCTGAATACATTAGCATGGAATAACACGACTGTAGGCACCATCAAT819250.32327371049401904No Hit
CAAGCGGTGGATTATGTGGATTAATTCGCTGTAGGCACCATCAATAGATC811850.320353691625962No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC649050.25611327652870686Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGATTCATCCCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGAT636820.25128735345352604No Hit
TTTTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGATCGGAAGA633180.24985101984815744No Hit
TCGAATCTTTGAACGCATATTGCGCTCGCTGTAGGCACCATCAATAGATC632000.24938539521784567No Hit
CGGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCGG602470.2377329415457207No Hit
AACCCGACTTCTGGAAGGGTCGTATTTATTAGATCTGTAGGCACCATCAA579720.2287558565121669No Hit
ATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC542540.21408473468590183No Hit
ATCGACCGACCATGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT517810.20432634730657065No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA513760.2027282288720259Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGA489980.19334470877981014No Hit
TAACCCTGCACCATCGACCGACCATGCTGTAGGCACCATCAATAGATCGG478980.18900414019215778No Hit
ACGAATCGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA467400.1844347052607928Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGATC458940.18109641341974378No Hit
ATGATAACTTCACGAATCGTACTGTAGGCACCATCAATAGATCGGAAGAG456280.18004678501582058No Hit
CCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA445960.175974542486314No Hit
GGGTTGAGTTGGCGGGACAAAAGCTGGGTCTGTAGGCACCATCAATAGAT444870.17554443159899208No Hit
ATGGCTCATTAAATCAGTTATAGTTTATTTGATGCTGTAGGCACCATCAA439940.17359906767743513No Hit
TGGGTTGAGTTGGCGGGACAAAAGCTGGGCTGTAGGCACCATCAATAGAT438540.1730466316753703No Hit
TGGGTTGAGTTGGCGGGACAAAAGCTGGGTCTGTAGGCACCATCAATAGA426770.16840222328658228No Hit
TCAGTGACGATCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG426000.1680983834854466No Hit
CTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAGAGC420210.1658136660197641No Hit
ACGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA411630.16242802252139527Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATTCATCCCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGA409160.16145336757489515No Hit
CCAAGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCG385460.1521014152542259No Hit
TAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT380690.1502191868757621TruSeq Adapter, Index 1 (100% over 35bp)
TAATACCCCATACAGCTGAGGACTGTAGGCACCATCAATAGATCGGAAGA369970.1459891054885227No Hit
TAATACCCCATACAGCTGAGGAGTGAAAGGTGCTGTAGGCACCATCAATA368110.14525515480006512No Hit
ACGAGACCTCAGCCTGCTAAATAGTCAGCATCGCCTGTAGGCACCATCAA361910.1428086525052065No Hit
AGGTAACCCGGCCGATGCCGCCGACTCGACCAAGGCTGTAGGCACCATCA355890.14043317769632768No Hit
ATCCCGTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGATCGGAAGA352020.1389060867477627No Hit
ACCCTGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAG344990.1361320688231085No Hit
ACAAGCGGTGGATTATGTGGATTAATTCGCTGTAGGCACCATCAATAGAT341820.13488119587557593No Hit
AGGTAACCCGGCCGATGCCGCCGACTCGACCAAGCTGTAGGCACCATCAA340660.13442346318815077No Hit
GCGGTGAATCATGATAACTTCACGAATCGTATCTGTAGGCACCATCAATA335730.13247809926659387No Hit
ACGAGACCTCAGCCTGCTAAATAGTCAGCATCGCACCTGTAGGCACCATC332920.13136928129102082No Hit
TAGCCGAGATATACTAAAGGCCGTCTGTAGGCACCATCAATAGATCGGAA321530.126874819817079No Hit
ACGTGCGAACCCTCAGTGACGATCTGTCTGTAGGCACCATCAATAGATCG320250.12636973547233396No Hit
TGTGCGGATGCTGGAGCATACTGTAGGCACCATCAATAGATCGGAAGAGC313940.12387982749159883No Hit
CTCAGTGACGATCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC309460.1221120322849913No Hit
TGCACCATCGACCGACCATCTGTAGGCACCATCAATAGATCGGAAGAGCA305120.1203994806785903No Hit
TCAAGAGCATGTCTGCCTCAGCGTCGGGCTGTAGGCACCATCAATAGATC299780.11829233192785724No Hit
TCACGAATCGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT296840.11713221632352105Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCAGTTAAAAAGCTCGTAGTTGGATTTCGGCTGTAGGCACCATCAATA293590.11584977560444194No Hit
GGGGAGGTAGTGACAATAAATAACACTGTAGGCACCATCAATAGATCGGA290690.11470544388587903No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC288230.11373473491082223Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCCAATGATGTCACCCGACTAAGGATACATCCGCCCTGTAGGCACCA287340.11338354345236672No Hit
ACCCAATGATGTCACCCGACTAAGGATACATCCGCCCTGTAGGCACCATC286440.11302840602246789No Hit
TTGTCCTAAGATGAGCTCAACGAGAACCTGTAGGCACCATCAATAGATCG286250.1129534325650448No Hit
ATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCA284990.11245624016318645No Hit
TCAGCCTGCTAAATAGTCAGCATCGCACTGTAGGCACCATCAATAGATCG282280.11138688190204662No Hit
ATTAAGGCGTACGGTGGAGACCTAGGCACTCAGACTGTAGGCACCATCAA279760.1103924970983299No Hit
TGCGGTGAATCATGATAACTTCACGAATCGTACTGTAGGCACCATCAATA276700.10918502983667387No Hit
GTGTTGCTAAGATTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA274390.10827351043326688No Hit
AGAGCATGTCTGCCTCAGCGTCGGCTGTAGGCACCATCAATAGATCGGAA272710.10761058723078906No Hit
AAGTGACCTGCCGAAGCTGTGATACTGTAGGCACCATCAATAGATCGGAA271440.10710944885748738No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTAT267040.10537322142242642TruSeq Adapter, Index 27 (97% over 43bp)
ACCTCAGCCTGCTAAATAGTCAGCATCGCACCTGTAGGCACCATCAATAG254820.10055124431868896No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)