FastQCFastQC Report
Fri 29 Sept 2023
SRR11712354_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11712354_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15462723
Total Bases472 Mbp
Sequences flagged as poor quality0
Sequence length18-50
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT11213327.251840442333475No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC10501706.791623959117679No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC6911364.469691399115149No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG4620242.987986009967326No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT3657162.365146164747309No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3655802.364266630140112No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG3170742.0505702650173583No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT3066011.9828396330969649No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2684211.7359232264588842No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2566241.6596300664507797No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2496351.6144310416735783No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAA2001371.2943192476512706No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1810631.1709645189919007No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA1710381.1061311775422737No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1562511.0105011905082955No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1527120.9876138892224868No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1495050.9668736871248357No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1141720.7383693027418262No Hit
TTACCCGTCGCCGCGCTCTCTCTCTCCCC1057140.6836700107736522No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCC1049320.6786126867822698No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1009420.6528086935270069No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGC979330.6333489903427747No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA776150.5019491068940445No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC758440.4904957555017962No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC737680.4770699184095842No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT730320.47231008406475367No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT703520.4549780785699905No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC630330.40764488893709083No Hit
TTACCCGTCGCCGCGCTCTCTCTCTCCC613350.3966636406795879No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG569320.3681887077715872No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCG525670.33995952718030326No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCC514910.33300085631748044No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT504910.3265336900880912No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCG502900.325233789675984No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT499320.32291854416586263No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA497700.32187086323670155No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGG478760.30962205039823837No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATA475000.30719039589598807No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG456750.2953878175273527No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC448720.29019468304515317No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT438640.2836757794859288No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT434830.2812117891525316No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC422990.2735546643369347No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT410150.26525082289839896No Hit
CTAAACCATTCGTAGACGACCTGCTT398560.25775537723853686No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA393490.2544765239602365No Hit
ATGGGGGGGGATGCGTGCATTTATCAGA371870.240494510572297No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCC368100.23805638890381725No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA363640.2351720327655097No Hit
ATTGATCATCGACACTTCGAACGCA341700.22098307005822973No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGA341550.2208860625647889No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCC307290.19872955106290138No Hit
GCCGCCGAGGGCGCACCACCGGCCCGTCTCGC297380.19232058932957669No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCC287870.18617031424542754No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCT287500.18593102909494014No Hit
TCAACTTCTTAGAGGGAC280460.18137814406945013No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGA267120.1727509443194449No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC262510.16976958068769646No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC250040.16170502439964812No Hit
TCTCGCCCGTCACGTTGAACGCACGTTC245050.1584779084511829No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCG234840.1518749317309765No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT232820.15056856415263986No Hit
ATGGGGGGGGGATGCGTGCATTTATCAGA230690.14919105774577995No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCC207610.13426483808834963No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCC200840.12988656655105313No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGC199540.12904583494123253No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCC198530.12839265115206422No Hit
TTACCCGTCGCCGCGCTCTCTCTCTCCCCC180560.11677115343785179No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC179440.11604683082016021No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGAAT177850.11501855138968732No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTC174280.11270977304579535No Hit
GTCTAGCGGTTAGGATTCCTGG171910.11117705464943012No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCC169600.10968313925044121No Hit
TATTCGAACGTCTGCCCTATC164890.10663710395639887No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGC164520.10639781880591148No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC161670.10455467643053556No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGA160320.10368160898956802No Hit
TTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC160060.10351346266760388No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)