FastQCFastQC Report
Wed 30 Aug 2023
SRR11728139_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11728139_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15977217
Total Bases456.3 Mbp
Sequences flagged as poor quality0
Sequence length6-49
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCAC14687979.193071609404816No Hit
TAAGGCTGAGGCGTGATGACGAGGCAC3643882.2806725351480175No Hit
AAGGCTGAGGCGTGATGACGAGGCACT3192321.998045091332239No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGC2901411.8159670736148856No Hit
AAAGGCTGAGGCGTGATGACGAGGCAC2784101.7425437734243705No Hit
ACGCTTTAGCAGCTTAATAACCTGCTT2159701.3517372894165487No Hit
TAGGCTGAGGCGTGATGACGAGGCAC816660.5111403318863353No Hit
TAAGGCTGAGGCGTGATGACGAGGCACT769930.4818924347087481No Hit
TACGCTTTAGCAGCTTAATAACCTGCTT751600.47041984846297075No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGC698420.43713495285192655No Hit
AAGGCTGAGGCGTGATGACGAGGCA674300.422038456384488No Hit
AAAGGCTGAGGCGTGATGACGAGGCACT585150.3662402532305845No Hit
ATGTACACGGAGTCGACCCGCAACGCGA575770.36036939349324726No Hit
AGCGGGGAGGAAGGGAGTAAAG556620.3483835764388754No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCA533310.33379405186773137No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGTG487510.3051282335340379No Hit
AGGTTAATGAGGCGAACCGGGGG462440.28943714039810564No Hit
AGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGG419230.26239238035009477No Hit
GCGGGAGTGGCGAAATTGGTAGACGCACCAGA364180.2279370681389631No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGG360380.22555868146498856No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGC352750.22078313138013964No Hit
CAAGGCTGAGGCGTGATGACGAGGCAC333890.2089788227824658No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCG327160.20476657480461083No Hit
TTAGGTTAATGAGGCGAACCGGGGG280490.17555623110082313No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGC276560.17309647856694943No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATG260630.163126031273156No Hit
AAGGCTGAGGCGTGATGACGAGGC237620.14872427407101No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGC235010.14709069796072746No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGC232280.14538201490284572No Hit
AAAACCATGCACCGAAGCTGCGGCAGCG215520.13489207788815788No Hit
AGGCTGAGGCGTGATGACGAGGCAC212170.13279534226768028No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCTACC211850.13259505707408242No Hit
GCGGGAGTGGCGAAATTGGTAGACGCACCAG204490.1279884976213317No Hit
GAAGGCTGAGGCGTGATGACGAGGCAC195540.12238677111289155No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGT182000.11391220385878217No Hit
TAAGGCTGAGGCGTGATGACGAGGCA178140.11149626371100799No Hit
TTACGCTTTAGCAGCTTAATAACCTGCTT177130.11086411356871476No Hit
AGTAGGTAGCTTAACCTTCGGGAGGG171710.10747178310215103No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCT171020.10703991815345563No Hit
TAGGCTGAGGCGTGATGACGAGGCACT170930.10698358794275624No Hit
AGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCG169160.10587576046566809No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCC169080.10582568916726863No Hit
AGGTAGCGCCTCGTGAATTCATCTCCGGGG166730.10435484477678435No Hit
AGTAGGTAGCTTAACCTTCGGGAGGGCGCT165640.10367262333609163No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGC165150.10336593663339493No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGT160010.10014885571122932No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)