FastQCFastQC Report
Fri 29 Sept 2023
SRR11791220.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11791220.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26221544
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGAGCTGTGATGGGGAGAAGACTGGAATTCTCGGGTGCCAAGGAAC300154811.446877422626219RNA PCR Primer, Index 1 (100% over 24bp)
AGGCTGAGCTGTGATGGGGAGAAGTGGAATTCTCGGGTGCCAAGGAACTC10358913.9505339578783003RNA PCR Primer, Index 1 (100% over 26bp)
TGCTTGTTGGGGTTGTAGGACACTGGAATTCTCGGGTGCCAAGGAACTCC7195072.7439535978506835RNA PCR Primer, Index 1 (100% over 27bp)
ACGGTCCTAAGGTAGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC7039912.6847808809427853RNA PCR Primer, Index 1 (100% over 33bp)
AAGGCTGAGCTGTGATGGGGAGAAGACTGGAATTCTCGGGTGCCAAGGAA5393842.057026085115354RNA PCR Primer, Index 1 (100% over 23bp)
TGCTTGTTGGGGTTGTAGGACACTTGGAATTCTCGGGTGCCAAGGAACTC5149831.9639690172325475RNA PCR Primer, Index 1 (100% over 26bp)
TGCTTGTTGGGGTTGTAGGACACTCTGGAATTCTCGGGTGCCAAGGAACT4300451.6400445374231205RNA PCR Primer, Index 1 (100% over 25bp)
ACGGTCCTAAGGTAGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4286991.6349113538089137RNA PCR Primer, Index 1 (100% over 32bp)
AACCGGAGGAAGGTGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4207721.604680487159719RNA PCR Primer, Index 1 (100% over 32bp)
AACTTGGGAGTCAGAACATGGGTGGAATTCTCGGGTGCCAAGGAACTCCA3645601.3903071459102485RNA PCR Primer, Index 1 (100% over 28bp)
TTGCTTGTTGGGGTTGTAGGACACTGGAATTCTCGGGTGCCAAGGAACTC3226521.2304843681211144RNA PCR Primer, Index 1 (100% over 26bp)
AAGGCTGAGCTGTGATGGGGAGAAGTGGAATTCTCGGGTGCCAAGGAACT2915281.1117880777729947RNA PCR Primer, Index 1 (100% over 25bp)
TTGCTTGTTGGGGTTGTAGGACACTTGGAATTCTCGGGTGCCAAGGAACT2685941.0243256461175587RNA PCR Primer, Index 1 (100% over 25bp)
AGAGTAGAACGTTGCCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2675851.020477665235884RNA PCR Primer, Index 1 (100% over 30bp)
AAGAACCTTACCAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2484870.9476444255151413RNA PCR Primer, Index 1 (100% over 33bp)
ACTTGGGAGTCAGAACATGGGTGGAATTCTCGGGTGCCAAGGAACTCCAG2332200.8894213094392915RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTTGTTGGGGTTGTAGGACACTCTGGAATTCTCGGGTGCCAAGGAAC2177950.8305956354057564RNA PCR Primer, Index 1 (100% over 24bp)
AGGCTGAGCTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC2121350.8090103313519601RNA PCR Primer, Index 1 (100% over 36bp)
AAACCGGAGGAAGGTGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1748410.6667837713904261RNA PCR Primer, Index 1 (100% over 31bp)
ACGCGAAGAACCTTACCAAATCTGGAATTCTCGGGTGCCAAGGAACTCCA1533540.5848397028031607RNA PCR Primer, Index 1 (100% over 28bp)
CGAAGAACCTTACCAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1275960.48660750106858697RNA PCR Primer, Index 1 (100% over 31bp)
AACGCGAAGAACCTTACCAAATCTGGAATTCTCGGGTGCCAAGGAACTCC1272860.4854252671009762RNA PCR Primer, Index 1 (100% over 27bp)
ACGCGAAGAACCTTACCAAATGGAATTCTCGGGTGCCAAGGAACTCCAGT1241060.47329783478806586RNA PCR Primer, Index 1 (100% over 30bp)
AAGAACCTTACCAAATCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1212880.4625509466566881RNA PCR Primer, Index 1 (100% over 32bp)
AGAACGTTGCCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT1018430.38839436762381346RNA PCR Primer, Index 1 (100% over 35bp)
AAATTCCGTCGGAATCTGGGAGGACTGGAATTCTCGGGTGCCAAGGAACT996040.3798555874512958RNA PCR Primer, Index 1 (100% over 25bp)
AGGCTGAGCTGTGATGGGGAGAAGACATGGAATTCTCGGGTGCCAAGGAA982710.3747719813905695RNA PCR Primer, Index 1 (100% over 23bp)
ACCCTTAAGCTTAGTTGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGT912100.3478437425347646RNA PCR Primer, Index 1 (100% over 30bp)
ACAAACCGGAGGAAGGTGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAG910110.34708482460071766RNA PCR Primer, Index 1 (100% over 29bp)
TTACCGAAGCTGTGGATTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA904980.34512841806721983RNA PCR Primer, Index 1 (100% over 28bp)
AAATTCCGTCGGAATCTGGGAGGATGGAATTCTCGGGTGCCAAGGAACTC895270.34142535618802616RNA PCR Primer, Index 1 (100% over 26bp)
ACGATGAGTGCTAAGTGTTAGGGGGTGGAATTCTCGGGTGCCAAGGAACT831000.31691497647888317RNA PCR Primer, Index 1 (100% over 25bp)
CGAAGAACCTTACCAAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC789340.3010272774173786RNA PCR Primer, Index 1 (100% over 33bp)
AGAAGGCACACCCGGAGAACTGATGGAATTCTCGGGTGCCAAGGAACTCC777720.29659580686781833RNA PCR Primer, Index 1 (100% over 27bp)
ACGGTCCTAAGGTAGCGTGGAATTCTCGGGTGCCCAGGAACTCCAGTCAC756680.2885718705199053RNA PCR Primer, Index 1 (96% over 33bp)
AACGATGAGTGCTAAGTGTTAGGGGGTGGAATTCTCGGGTGCCAAGGAAC724610.27634146944207405RNA PCR Primer, Index 1 (100% over 24bp)
AAGGCTGAGCTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT722350.2754795827431062RNA PCR Primer, Index 1 (100% over 35bp)
AACTTGGGAGTCAGAACATGGGTGTGGAATTCTCGGGTGCCAAGGAACTC699220.26665859188154595RNA PCR Primer, Index 1 (100% over 26bp)
AACGGTCCTAAGGTAGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA668890.25509176728876076RNA PCR Primer, Index 1 (100% over 32bp)
CGCGAAGAACCTTACCAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAG641920.24480633177054717RNA PCR Primer, Index 1 (100% over 29bp)
AACGCGAAGAACCTTACCAAATGGAATTCTCGGGTGCCAAGGAACTCCAG624090.23800657962780528RNA PCR Primer, Index 1 (100% over 29bp)
ACTTGGGAGTCAGAACATGGGTGTGGAATTCTCGGGTGCCAAGGAACTCC585740.22338120135107226RNA PCR Primer, Index 1 (100% over 27bp)
TTGGGGTTGTAGGACACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA549190.20944228150714542RNA PCR Primer, Index 1 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC545360.20798165050845216RNA PCR Primer, Index 1 (100% over 50bp)
TTGGGGTTGTAGGACACTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC541530.20652101950975882RNA PCR Primer, Index 1 (100% over 31bp)
ATGCGCAGAGATATGGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC457020.17429179608950562RNA PCR Primer, Index 1 (100% over 31bp)
ATTACCGAAGCTGTGGATTGTCCTGGAATTCTCGGGTGCCAAGGAACTCC438530.1672403425214015RNA PCR Primer, Index 1 (100% over 27bp)
ACCCTTAAGCTTAGTTGCCATTGGAATTCTCGGGTGCCAAGGAACTCCAG422920.16128722244578733RNA PCR Primer, Index 1 (100% over 29bp)
AGAACCTGAAACCGTGTGCTTACAATGGAATTCTCGGGTGCCAAGGAACT413310.157622297146194RNA PCR Primer, Index 1 (100% over 25bp)
TTGTTGGGGTTGTAGGACACTCTGGAATTCTCGGGTGCCAAGGAACTCCA404560.15428534643116362RNA PCR Primer, Index 1 (100% over 28bp)
TTCCGCTAGAGTAGAACGTTGCCAGGCTGGAATTCTCGGGTGCCAAGGAA396890.151360270775817RNA PCR Primer, Index 1 (100% over 23bp)
AGTAGAACGTTGCCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA386100.14724533383693958RNA PCR Primer, Index 1 (100% over 32bp)
AACGGTCCTAAGGTAGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC379550.14474738787311683RNA PCR Primer, Index 1 (100% over 31bp)
AACCCTTAAGCTTAGTTGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG361960.13803916352141582RNA PCR Primer, Index 1 (100% over 29bp)
ACAAACCGGAGGAAGGTGGGGATGGAATTCTCGGGTGCCAAGGAACTCCA361210.1377531391744132RNA PCR Primer, Index 1 (100% over 28bp)
CGCGAAGAACCTTACCAAATGGAATTCTCGGGTGCCAAGGAACTCCAGTC357310.1362658125699997RNA PCR Primer, Index 1 (100% over 31bp)
TAAGGCTGAGCTGTGATGGGGAGAAGTGGAATTCTCGGGTGCCAAGGAAC356880.13610182527771822RNA PCR Primer, Index 1 (100% over 24bp)
AACCTGGAGATAGCTGGTTCTCTCCGATGGAATTCTCGGGTGCCAAGGAA343550.13101821921699194RNA PCR Primer, Index 1 (100% over 23bp)
AACGCGAAGAACCTTACCAAATCTTGGAATTCTCGGGTGCCAAGGAACTC339120.12932876874069657RNA PCR Primer, Index 1 (100% over 26bp)
AGGAAATTTGAGAGGAGCTGTCCTTGGAATTCTCGGGTGCCAAGGAACTC333650.1272426978365576RNA PCR Primer, Index 1 (100% over 26bp)
TTGTTGGGGTTGTAGGACACTTGGAATTCTCGGGTGCCAAGGAACTCCAG331600.1264608979547505RNA PCR Primer, Index 1 (100% over 29bp)
AGAAGGCACACCCGGAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCA327060.12472949724089473RNA PCR Primer, Index 1 (100% over 28bp)
TGCGCAGAGATATGGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA325310.12406210709788867RNA PCR Primer, Index 1 (100% over 32bp)
AGAACCTGAAACCGTGTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG321310.12253664391387478RNA PCR Primer, Index 1 (100% over 29bp)
AAGGACATGTGGAGCGCTTAGAAGTGGAATTCTCGGGTGCCAAGGAACTC317940.12125144118134311RNA PCR Primer, Index 1 (100% over 26bp)
ACGCGAAGAACCTTACCAAATCTTGGAATTCTCGGGTGCCAAGGAACTCC314550.11995861113289134RNA PCR Primer, Index 1 (100% over 27bp)
ACCTCAGGAGGTGAATGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC305550.11652631896886011RNA PCR Primer, Index 1 (100% over 31bp)
AACCGGAGGAAGGTGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC302930.11552714058333101RNA PCR Primer, Index 1 (100% over 31bp)
TTGGGGTTGTAGGACACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC298540.11385294473887579RNA PCR Primer, Index 1 (100% over 33bp)
AACCGGAGGAAGGTGGGGATGACGTCTGGAATTCTCGGGTGCCAAGGAAC297740.11354785210207301RNA PCR Primer, Index 1 (100% over 24bp)
ATCCTGAGTACGACGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC294770.11241519568794271RNA PCR Primer, Index 1 (100% over 31bp)
AGAACCTGAAACCGTGTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT292640.11160288654245533RNA PCR Primer, Index 1 (100% over 30bp)
TAGAGTAGAACGTTGCCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG292130.11140838998649355RNA PCR Primer, Index 1 (100% over 29bp)
AACCGGAGGAAGGTGGGGATGACGTGGAATTCTCGGGTGCCAAGGAACTC291370.11111855198153091RNA PCR Primer, Index 1 (100% over 26bp)
AGGATGAACGCTGGCGGCGTGCCTGGAATTCTCGGGTGCCAAGGAACTCC280810.10709132917573429RNA PCR Primer, Index 1 (100% over 27bp)
ACGGTCCTAAGGTAGCGAAATGGAATTCTCGGGTGCCAAGGAACTCCAGT279690.10666419948421038RNA PCR Primer, Index 1 (100% over 30bp)
ATCCTGAGTACGACGGAGCACGTGGAATTCTCGGGTGCCAAGGAACTCCA272610.10396412964850583RNA PCR Primer, Index 1 (100% over 28bp)
AGAAGGGGTGCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT271770.1036437823798629RNA PCR Primer, Index 1 (100% over 35bp)
ACCGGAGGAAGGTGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC271580.10357132287862225RNA PCR Primer, Index 1 (100% over 33bp)
TAAGGCTGAGCTGTGATGGGGAGAAGACTGGAATTCTCGGGTGCCAAGGA270550.10317851610873868RNA PCR Primer, Index 1 (100% over 22bp)
TAGAACGTTGCCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA270180.1030374107642174RNA PCR Primer, Index 1 (100% over 34bp)
ACATATTACCGAAGCTGTGGATTGTCCTGGAATTCTCGGGTGCCAAGGAA264480.10086362572699761RNA PCR Primer, Index 1 (100% over 23bp)
GATGGGGAGAAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC263260.10039835945587339RNA PCR Primer, Index 1 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph