FastQCFastQC Report
Fri 29 Sept 2023
SRR11791221.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11791221.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20114374
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGAGCTGTGATGGGGAGAAGACTGGAATTCTCGGGTGCCAAGGAAC15851037.880449075869824RNA PCR Primer, Index 1 (100% over 24bp)
AGGCTGAGCTGTGATGGGGAGAAGTGGAATTCTCGGGTGCCAAGGAACTC10202255.072119072659184RNA PCR Primer, Index 1 (100% over 26bp)
TGCTTGTTGGGGTTGTAGGACACTGGAATTCTCGGGTGCCAAGGAACTCC7077213.518483846427435RNA PCR Primer, Index 1 (100% over 27bp)
TGCTTGTTGGGGTTGTAGGACACTTGGAATTCTCGGGTGCCAAGGAACTC5111852.5413915441763186RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTTGTTGGGGTTGTAGGACACTGGAATTCTCGGGTGCCAAGGAACTC3512781.7464028460443264RNA PCR Primer, Index 1 (100% over 26bp)
ACGGTCCTAAGGTAGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3470121.7251941323155273RNA PCR Primer, Index 1 (100% over 33bp)
AACCGGAGGAAGGTGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3246731.6141342504618836RNA PCR Primer, Index 1 (100% over 32bp)
TTGCTTGTTGGGGTTGTAGGACACTTGGAATTCTCGGGTGCCAAGGAACT3150801.5664419882020688RNA PCR Primer, Index 1 (100% over 25bp)
AAGGCTGAGCTGTGATGGGGAGAAGTGGAATTCTCGGGTGCCAAGGAACT3052031.5173378003213025RNA PCR Primer, Index 1 (100% over 25bp)
TGCTTGTTGGGGTTGTAGGACACTCTGGAATTCTCGGGTGCCAAGGAACT2793721.3889171992128615RNA PCR Primer, Index 1 (100% over 25bp)
AAGGCTGAGCTGTGATGGGGAGAAGACTGGAATTCTCGGGTGCCAAGGAA2551641.2685654547340126RNA PCR Primer, Index 1 (100% over 23bp)
AGGCTGAGCTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA2448461.2172688048855014RNA PCR Primer, Index 3 (100% over 36bp)
AACTTGGGAGTCAGAACATGGGTGGAATTCTCGGGTGCCAAGGAACTCCA1907820.9484858937195858RNA PCR Primer, Index 1 (100% over 28bp)
AAGAACCTTACCAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1701460.8458925940225632RNA PCR Primer, Index 1 (100% over 33bp)
AAATTCCGTCGGAATCTGGGAGGATGGAATTCTCGGGTGCCAAGGAACTC1593880.7924084537753947RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTTGTTGGGGTTGTAGGACACTCTGGAATTCTCGGGTGCCAAGGAAC1587760.789365853493626RNA PCR Primer, Index 1 (100% over 24bp)
ACGGTCCTAAGGTAGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1580900.7859553570993559RNA PCR Primer, Index 1 (100% over 32bp)
AAATTCCGTCGGAATCTGGGAGGACTGGAATTCTCGGGTGCCAAGGAACT1529870.7605854400440202RNA PCR Primer, Index 1 (100% over 25bp)
AAACCGGAGGAAGGTGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1429940.7109045501490625RNA PCR Primer, Index 1 (100% over 31bp)
ACGCGAAGAACCTTACCAAATCTGGAATTCTCGGGTGCCAAGGAACTCCA1404780.6983960823240136RNA PCR Primer, Index 1 (100% over 28bp)
TTACCGAAGCTGTGGATTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA1350960.6716390974931657RNA PCR Primer, Index 1 (100% over 28bp)
AACGCGAAGAACCTTACCAAATCTGGAATTCTCGGGTGCCAAGGAACTCC1202340.5977516377094311RNA PCR Primer, Index 1 (100% over 27bp)
ACTTGGGAGTCAGAACATGGGTGGAATTCTCGGGTGCCAAGGAACTCCAG1172230.5827822431858929RNA PCR Primer, Index 1 (100% over 29bp)
AGAAGGCACACCCGGAGAACTGATGGAATTCTCGGGTGCCAAGGAACTCC1136320.5649293385913974RNA PCR Primer, Index 1 (100% over 27bp)
CGAAGAACCTTACCAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC976270.48535937534024176RNA PCR Primer, Index 1 (100% over 31bp)
ACGCGAAGAACCTTACCAAATGGAATTCTCGGGTGCCAAGGAACTCCAGT946890.47075290536011705RNA PCR Primer, Index 1 (100% over 30bp)
AGAGTAGAACGTTGCCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT842410.41880995153018435RNA PCR Primer, Index 1 (100% over 30bp)
AAGGCTGAGCTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT805700.40055932140866024RNA PCR Primer, Index 3 (100% over 35bp)
ACCCTTAAGCTTAGTTGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGT793190.3943398884797509RNA PCR Primer, Index 1 (100% over 30bp)
ACAAACCGGAGGAAGGTGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAG791510.39350466487299085RNA PCR Primer, Index 1 (100% over 29bp)
AAGAACCTTACCAAATCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA709360.35266322481624335RNA PCR Primer, Index 1 (100% over 32bp)
ACGATGAGTGCTAAGTGTTAGGGGGTGGAATTCTCGGGTGCCAAGGAACT700570.3482932155880168RNA PCR Primer, Index 1 (100% over 25bp)
ATTACCGAAGCTGTGGATTGTCCTGGAATTCTCGGGTGCCAAGGAACTCC635040.3157145233552881RNA PCR Primer, Index 1 (100% over 27bp)
CGCGAAGAACCTTACCAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAG532250.26461176470120323RNA PCR Primer, Index 1 (100% over 29bp)
AGAACCTGAAACCGTGTGCTTACAATGGAATTCTCGGGTGCCAAGGAACT520680.25885965926655236RNA PCR Primer, Index 1 (100% over 25bp)
CGAAGAACCTTACCAAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC517980.2575173356128309RNA PCR Primer, Index 1 (100% over 33bp)
TTGGGGTTGTAGGACACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA516750.2569058326150245RNA PCR Primer, Index 1 (100% over 32bp)
ACCCTTAAGCTTAGTTGCCATTGGAATTCTCGGGTGCCAAGGAACTCCAG510210.25365442643156577RNA PCR Primer, Index 1 (100% over 29bp)
AACTTGGGAGTCAGAACATGGGTGTGGAATTCTCGGGTGCCAAGGAACTC503360.2502489016063836RNA PCR Primer, Index 1 (100% over 26bp)
AACGATGAGTGCTAAGTGTTAGGGGGTGGAATTCTCGGGTGCCAAGGAAC499390.24827518867850426RNA PCR Primer, Index 1 (100% over 24bp)
AGAAGGCACACCCGGAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCA496650.24691297874843135RNA PCR Primer, Index 1 (100% over 28bp)
AACGCGAAGAACCTTACCAAATGGAATTCTCGGGTGCCAAGGAACTCCAG451550.22449120216219506RNA PCR Primer, Index 1 (100% over 29bp)
ACTTGGGAGTCAGAACATGGGTGTGGAATTCTCGGGTGCCAAGGAACTCC447150.22230371176353786RNA PCR Primer, Index 1 (100% over 27bp)
TTCCGCTAGAGTAGAACGTTGCCAGGCTGGAATTCTCGGGTGCCAAGGAA428550.21305659326012333RNA PCR Primer, Index 1 (100% over 23bp)
TTGGGGTTGTAGGACACTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC419660.20863686834101822RNA PCR Primer, Index 1 (100% over 31bp)
AACCCTTAAGCTTAGTTGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG413340.20549483667749244RNA PCR Primer, Index 1 (100% over 29bp)
AGGAAATTTGAGAGGAGCTGTCCTTGGAATTCTCGGGTGCCAAGGAACTC392360.1950644847311679RNA PCR Primer, Index 1 (100% over 26bp)
CGCTAGAGTAGAACGTTGCCAGGCTGGAATTCTCGGGTGCCAAGGAACTC379440.18864121746965629RNA PCR Primer, Index 1 (100% over 26bp)
ACGGTCCTAAGGTAGCGTGGAATTCTCGGGTGCCCAGGAACTCCAGTCAC375390.1866277319890741RNA PCR Primer, Index 1 (96% over 33bp)
AACCCTTAAGCTTAGTTGCCATTGGAATTCTCGGGTGCCAAGGAACTCCA370360.1841270327378819RNA PCR Primer, Index 1 (100% over 28bp)
AGGATGAACGCTGGCGGCGTGCCTGGAATTCTCGGGTGCCAAGGAACTCC348240.1731299219155416RNA PCR Primer, Index 1 (100% over 27bp)
ACATATTACCGAAGCTGTGGATTGTCCTGGAATTCTCGGGTGCCAAGGAA348200.17311003563919017RNA PCR Primer, Index 1 (100% over 23bp)
AGAACGTTGCCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT347890.1729559169974666RNA PCR Primer, Index 3 (100% over 35bp)
AACGGTCCTAAGGTAGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA343750.1708976873950937RNA PCR Primer, Index 1 (100% over 32bp)
ACAAACCGGAGGAAGGTGGGGATGGAATTCTCGGGTGCCAAGGAACTCCA334400.16624927029794712RNA PCR Primer, Index 1 (100% over 28bp)
AGGCTGAGCTGTGATGGGGAGAAGACATGGAATTCTCGGGTGCCAAGGAA330980.16454899366989995RNA PCR Primer, Index 1 (100% over 23bp)
TAGAGTAGAACGTTGCCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG323890.16102415118660912RNA PCR Primer, Index 1 (100% over 29bp)
ATCCTGAGTACGACGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC323800.16097940706481842RNA PCR Primer, Index 1 (100% over 31bp)
AGTAGAACGTTGCCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA320150.15916478434775053RNA PCR Primer, Index 1 (100% over 32bp)
AACGCGAAGAACCTTACCAAATCTTGGAATTCTCGGGTGCCAAGGAACTC314230.15622161544773902RNA PCR Primer, Index 1 (100% over 26bp)
CCGCTAGAGTAGAACGTTGCCAGGCTGGAATTCTCGGGTGCCAAGGAACT308350.15329833282407895RNA PCR Primer, Index 1 (100% over 25bp)
GAGTAGAACGTTGCCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC278280.13834882457689213RNA PCR Primer, Index 1 (100% over 31bp)
TAGGAAATTTGAGAGGAGCTGTCCTTGGAATTCTCGGGTGCCAAGGAACT272770.13560949000948277RNA PCR Primer, Index 1 (100% over 25bp)
GCTAGAGTAGAACGTTGCCAGGCTGGAATTCTCGGGTGCCAAGGAACTCC270200.13433179675390344RNA PCR Primer, Index 1 (100% over 27bp)
AGAACCTGAAACCGTGTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG267710.133093876051027RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGAGGAAGGTGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC267590.1330342172219727RNA PCR Primer, Index 1 (100% over 33bp)
TCCGCTAGAGTAGAACGTTGCCAGGCTGGAATTCTCGGGTGCCAAGGAAC267580.13302924565288485RNA PCR Primer, Index 1 (100% over 24bp)
ACGCGAAGAACCTTACCAAATCTTGGAATTCTCGGGTGCCAAGGAACTCC266290.13238791324055127RNA PCR Primer, Index 1 (100% over 27bp)
AAGGACATGTGGAGCGCTTAGAAGTGGAATTCTCGGGTGCCAAGGAACTC266050.1322685955824427RNA PCR Primer, Index 1 (100% over 26bp)
AGAACCTGAAACCGTGTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT264690.13159246218649412RNA PCR Primer, Index 1 (100% over 30bp)
TTGGGGTTGTAGGACACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC264380.13143834354477052RNA PCR Primer, Index 1 (100% over 33bp)
TTGTTGGGGTTGTAGGACACTTGGAATTCTCGGGTGCCAAGGAACTCCAG262510.1305086601253412RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC257370.12795327361418257RNA PCR Primer, Index 3 (100% over 50bp)
AACCGGAGGAAGGTGGGGATGACGTCTGGAATTCTCGGGTGCCAAGGAAC256550.1275456049489783RNA PCR Primer, Index 1 (100% over 24bp)
AACCGGAGGAAGGTGGGGATGACGTGGAATTCTCGGGTGCCAAGGAACTC255960.1272522823727947RNA PCR Primer, Index 1 (100% over 26bp)
AATTCCGTCGGAATCTGGGAGGATGGAATTCTCGGGTGCCAAGGAACTCC254940.12674518232583326RNA PCR Primer, Index 1 (100% over 27bp)
ATGCGCAGAGATATGGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC239240.11893981885789734RNA PCR Primer, Index 1 (100% over 31bp)
AGGAAATTTGAGAGGAGCTGTCCTGGAATTCTCGGGTGCCAAGGAACTCC238160.11840288939640876RNA PCR Primer, Index 1 (100% over 27bp)
ACCGAAGCTGTGGATTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT237360.11800516386938018RNA PCR Primer, Index 1 (100% over 30bp)
TTGTTGGGGTTGTAGGACACTCTGGAATTCTCGGGTGCCAAGGAACTCCA234500.11658329511025299RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTTAAGCTTAGTTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC229270.11398316447730364RNA PCR Primer, Index 1 (100% over 31bp)
AACCGGAGGAAGGTGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC228810.11375447229926221RNA PCR Primer, Index 1 (100% over 31bp)
CGCGAAGAACCTTACCAAATGGAATTCTCGGGTGCCAAGGAACTCCAGTC225490.11210391136209359RNA PCR Primer, Index 1 (100% over 31bp)
ATCCTGAGTACGACGGAGCACGTGGAATTCTCGGGTGCCAAGGAACTCCA223040.11088587693556856RNA PCR Primer, Index 1 (100% over 28bp)
CTAGAGTAGAACGTTGCCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA222680.1107069004484057RNA PCR Primer, Index 1 (100% over 28bp)
AACCTGGAGATAGCTGGTTCTCTCCGATGGAATTCTCGGGTGCCAAGGAA218880.10881770419501995RNA PCR Primer, Index 1 (100% over 23bp)
ATTCCGTCGGAATCTGGGAGGATGGAATTCTCGGGTGCCAAGGAACTCCA216750.10775875997930634RNA PCR Primer, Index 1 (100% over 28bp)
AGAAGGGGTGCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT210290.10454712634855054RNA PCR Primer, Index 3 (100% over 35bp)
TGCGCAGAGATATGGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA209260.10403505473250124RNA PCR Primer, Index 1 (100% over 32bp)
AGGACATGTGGAGCGCTTAGAAGTGGAATTCTCGGGTGCCAAGGAACTCC203590.10121617505968616RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph