FastQCFastQC Report
Fri 29 Sept 2023
SRR11798518_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11798518_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23398833
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG289476912.371424677461478TruSeq Adapter, Index 7 (100% over 49bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC3732171.595023991153747Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC2952121.2616526644726256Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATAACCTCGGGCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1646110.7035008968182302Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA1599290.6834913518977634TruSeq Adapter, Index 7 (100% over 35bp)
AGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1549840.6623578192980821Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTC1442330.6164110834074502No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCT1252090.5351078833717904Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT968290.41381978323448865No Hit
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC965340.4125590365981073Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC925470.39551972527860685No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG867930.3709287552930524Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC806830.34481634190901744TruSeq Adapter, Index 7 (100% over 50bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG767270.3279095158292723Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC753680.322101533867095TruSeq Adapter, Index 7 (100% over 50bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG729940.3119557287322834TruSeq Adapter, Index 7 (100% over 50bp)
GGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC709800.30334846186559816Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGA650550.2780266862026837Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCCGGCGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG649780.2776976099620011TruSeq Adapter, Index 7 (100% over 36bp)
GGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC645190.27573597367013986Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTG628370.2685475809840602Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAA595750.25460671478786995Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC550680.2353450704144091Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCC546900.23372960523287636Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG537320.22963538395269542Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA517530.2211776971954114Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCAGATCGGAAGAGCACACGT516990.22094691645519246No Hit
GACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGA492010.21027117036135945Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC491010.20984379862021324No Hit
CCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA460920.19698418292912298Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCTTTCGCCGAATCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA435960.18631698427011295Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT432850.18498785815514815Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTTTCGCCGAATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA417370.17837214360220444Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG409970.1752095927177223Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCGGGGCGCGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC402280.17192310402830774Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACCTCGGGCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG400180.17102562337190064TruSeq Adapter, Index 7 (100% over 36bp)
GGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA392890.16791008337894459TruSeq Adapter, Index 7 (100% over 37bp)
GCGGGGCGCGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA388250.16592707850002605TruSeq Adapter, Index 7 (100% over 35bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT374750.16015755999455186No Hit
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT364150.15562741953840176Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTTTCGCCGAATCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC351950.15041348429641768Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCAGATCGGAAGAGCACACG345560.14768257887049324No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC336340.14374221141712495TruSeq Adapter, Index 7 (98% over 50bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC332520.14210965136594633Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT332470.14208828277888902Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC323700.13834023260903655Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCGCCTGGATACCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC321480.13739146734369187Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTC319110.1363785963171753Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA316710.13535290413842435Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGA315690.1349169849624552Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA314930.13459218243918403No Hit
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA314700.13449388693872041Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT313000.13376735497877182Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC307500.1314168104024675Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC307090.13124158798859753Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAAC305050.13036974963665923Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC298910.12774568714602133Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGTGGGGTCTCCCCATGCGAGAGTAGATCGGAAGAGCACACGTCTGAA294320.12578405085416014Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAAC293390.12538659513489414Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC285820.12215139105441711Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC285300.12192915774902108Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT284140.12143340652929144Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC282600.12077525404792623Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA281870.12046327267688949Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA277070.11841188831938755Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC273910.11706139361736545No Hit
AGATAACCTCGGGCCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC272730.11655709496281288Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC271110.11586475274215598No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC270900.11577500467651529No Hit
GAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCA266230.11377917864536236Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTCCCGGGGCTACGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAG265790.11359113507925801Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCC251220.10736432881075736Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTCCGCCCGGAGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA235990.1008554571931002Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT235170.10050501236536027Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)