FastQCFastQC Report
Fri 29 Sept 2023
SRR11798519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11798519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25835345
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG322176612.47038117741412TruSeq Adapter, Index 7 (100% over 49bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC4403431.7044208234881322Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC3326171.287449422486907Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATAACCTCGGGCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1931410.7475843655271489Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA1876270.7262415113868229TruSeq Adapter, Index 7 (100% over 35bp)
AGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1807540.6996384217048388Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTC1621560.6276517693106092No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCT1396250.540441786242839Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1100110.4258158735639102Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1095940.42420180570454935No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC1051660.40706249519795457No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG973330.376743565839744Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC907360.35120878006467493TruSeq Adapter, Index 7 (100% over 50bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG861430.3334308096137288Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC844870.32702098617223807TruSeq Adapter, Index 7 (100% over 50bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG817160.31629536977346345TruSeq Adapter, Index 7 (100% over 50bp)
GGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC798220.3089643277455749Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCCGGCGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG764940.296082750201323TruSeq Adapter, Index 7 (100% over 36bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGA729350.28230704873497914Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTG722270.2795666169737621Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC712700.27586238929652385Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAA663790.25693096027941564Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCC655660.25378410855361133Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC635370.24593052657125347Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG609410.23588227678012427Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA584950.22641462693840547Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCAGATCGGAAGAGCACACGT576950.22331809387488344No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC559840.21669538378527556No Hit
GACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGA553300.21416396800584625Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA551840.21359885072175347Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCTTTCGCCGAATCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA503450.19486869635377424Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT490570.18988327812150368Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTTTCGCCGAATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA472010.18269932141413245Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG467500.18095365089957188Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCTCGGGCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG466910.18072528158613713TruSeq Adapter, Index 7 (100% over 36bp)
GGCGGGGCGCGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC452260.1750547554135623Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT439010.16992612252710387No Hit
GGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA438010.1695390558941636TruSeq Adapter, Index 7 (100% over 37bp)
GCGGGGCGCGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA427410.16543614958499683TruSeq Adapter, Index 7 (100% over 35bp)
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT413160.15992045006559813Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTTTCGCCGAATCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC402600.15583302642174895Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC394470.15268617469594464Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT390760.1512501574877363Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCAGATCGGAAGAGCACACG384270.14873809503995397No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC381640.1477201097953211TruSeq Adapter, Index 7 (98% over 50bp)
CGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTC379880.14703887252134623Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA370600.14344689416766063No Hit
GCCGCCTGGATACCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC366790.14197217029615822Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA365100.14131802768648918Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC362300.14023424111425647Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAAC362240.14021101711628003Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA360480.1395297798423052Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT360400.13949881451166996Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC359550.13916980787367075Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC358570.13879048257338927Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGA349480.13527204687996233Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC338870.13116526990446614Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC338400.1309833485869842Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGTGTGGGGTCTCCCCATGCGAGAGTAGATCGGAAGAGCACACGTCTGAA332900.1288544821058128Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC332480.12869191411997788Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAAC322320.12475931712930483Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC319540.12368327188973091Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT318640.12333491192008468Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA318190.12316073193526156Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA316880.12265367464610981Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC313770.12144989741766561No Hit
AGATAACCTCGGGCCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC309570.11982421755931652Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC309170.1196693909061404No Hit
GAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCA309150.1196616495734816Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTCCCGGGGCTACGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAG300930.11647996185071266Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCC299420.11589549123497285Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC298840.1156709925878675No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT268390.10388481361483658Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCAGATCGGAAGAGCACACGTCTGA264420.10234815908206374Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCCGGAGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA263470.10198044578077049Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA262800.10172111113670051TruSeq Adapter, Index 7 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)