FastQCFastQC Report
Fri 29 Sept 2023
SRR11798520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11798520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38383134
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG477131212.430751485795819TruSeq Adapter, Index 7 (100% over 49bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC6577251.7135781564892538Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC4940001.2870236182381563Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATAACCTCGGGCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2847080.7417528751039454Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA2806730.7312404453476884TruSeq Adapter, Index 7 (100% over 35bp)
AGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2676310.6972619797018138Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTC2414380.6290210695145425No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCT2069200.5390909455178934Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1648470.4294776971572983No Hit
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1625170.4234073226016406Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC1573350.40990660116498046No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG1451880.3782598888355495Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1336930.34831183925731546TruSeq Adapter, Index 7 (100% over 50bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1290280.3361580635911596Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1251500.3260546676568932TruSeq Adapter, Index 7 (100% over 50bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG1209900.3152165740296246TruSeq Adapter, Index 7 (100% over 50bp)
GGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1180400.30753090667374894Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCCGGCGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG1141900.2975004594465892TruSeq Adapter, Index 7 (100% over 36bp)
GACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTG1075680.2802480902158745Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGA1071670.27920336051766903Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1068090.2782706591910916Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAA986430.2569956898256406Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCC965120.25144377215263347Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC935910.24383365881483257Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG898200.23400903115415225Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA869320.2264848930782984Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCAGATCGGAAGAGCACACGT861040.22432769559671703No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC843580.21977882264642587No Hit
GACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGA825310.2150189195077192Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA817730.21304409379390438Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCTTTCGCCGAATCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA751380.19575785552060446Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT734740.191422618069697Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTTTCGCCGAATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA696880.18155891074449523Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCTCGGGCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG693760.18074605372245006TruSeq Adapter, Index 7 (100% over 36bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG691750.1802223862178633Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCGGGGCGCGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC663700.1729144889523612Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT660420.17205994695482657No Hit
GGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA650890.1695770856022335TruSeq Adapter, Index 7 (100% over 37bp)
GCGGGGCGCGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA638670.16639339559922336TruSeq Adapter, Index 7 (100% over 35bp)
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT606350.15797303055034537Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTTTCGCCGAATCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC604930.1576030763928761Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC586340.15275980330319042Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCAGATCGGAAGAGCACACG574800.14975327444601058No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT573830.14950055928210554Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTC567740.14791392490253663Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC559480.14576193804289145TruSeq Adapter, Index 7 (98% over 50bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA553950.14432120107753577No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA548680.14294820219735055Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGCCTGGATACCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC547790.14271632952119023Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAAC539350.14051744706411937Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC538240.140228257546661Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT534990.1393815314820306Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC532520.13873801967291155Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC532260.1386702815877411Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA527430.137411916390152Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGA523540.13639845042356366Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC504020.13131288341384525Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC503480.1311721966215682Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC497110.12951261353489268Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGTGGGGTCTCCCCATGCGAGAGTAGATCGGAAGAGCACACGTCTGAA491150.127959848197909Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAAC479990.12505232115751674Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT475640.12391901088639609Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA473030.12323902472372371Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC472930.12321297161404278Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC471480.12283520152366921No Hit
AACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA464860.12111048566279137Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCA462510.12049823758528942Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATAACCTCGGGCCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC461850.12032628706139525Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC454820.11849475345082555No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC446010.11619947448793524No Hit
CCTCCCGGGGCTACGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAG445950.11618384262212669Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCC438290.11418817442056713Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT395150.10294886290421203Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCAGATCGGAAGAGCACACGTCTGA393740.10258151405771088Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA391550.1020109509556984TruSeq Adapter, Index 7 (100% over 35bp)
AGTCCGCCCGGAGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA387790.10103135403169528Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGAGGGACGGCCGGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT385620.100466001551619Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)