FastQCFastQC Report
Fri 29 Sept 2023
SRR11798521_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11798521_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14614257
Total Bases745.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG175824312.03101190843982TruSeq Adapter, Index 7 (100% over 49bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2256761.544218087857631Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC1770761.2116661148082999Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATAACCTCGGGCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA979950.6705438394849632Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA955580.6538683424001644TruSeq Adapter, Index 7 (100% over 35bp)
AGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC921410.6304870647888565Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTC792510.5422855229656903No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCT702590.4807565653183737Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC568370.38891474263795967Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT564750.38643770942306543No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC537570.367839432411788No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG486030.3325725009489021Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC463830.31738185526640184TruSeq Adapter, Index 7 (100% over 50bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG458590.31379631547467657Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG424960.29078454005564564TruSeq Adapter, Index 7 (100% over 50bp)
GGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC420370.2876437714212909Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC415950.2846193275511715TruSeq Adapter, Index 7 (100% over 50bp)
AACCCGGCGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG391830.26811489629613056TruSeq Adapter, Index 7 (100% over 36bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGA375250.25676981046658753Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTG367900.25174047507170566Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC367180.25124780548200293Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAA342090.2340796388075015Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCC337420.23088412910762415Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG318810.2181499887404471Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC308630.21118418815270595Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCAGATCGGAAGAGCACACGT301690.20643540071862704No Hit
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA295300.20206295811001546Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC289790.1982926672221516No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCCCGTATG288360.19731417067593654TruSeq Adapter, Index 7 (97% over 49bp)
GACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGA285230.1951724264873678Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA276250.1890277418824645Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT257610.17627307361571648Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCTTTCGCCGAATCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA256100.17523983600397885Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG244410.16724079780450007Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCTTTCGCCGAATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA244050.16699446300964874Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCTCGGGCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG236270.1616708943875833TruSeq Adapter, Index 7 (100% over 36bp)
GGCGGGGCGCGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC232090.15881067371403143Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA225530.1543219063411845TruSeq Adapter, Index 7 (100% over 37bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT223760.15311076026649867No Hit
GCGGGGCGCGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA221070.15127009193830382TruSeq Adapter, Index 7 (100% over 35bp)
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT211560.1447627477743138Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTTTCGCCGAATCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC206690.1414303854106302Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT201770.13806380988099498Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCAGATCGGAAGAGCACACG200910.13747534342662784No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC197460.135114634975969TruSeq Adapter, Index 7 (98% over 50bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC197080.13485461491473702Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTC195090.13349293090986425Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC192430.13167279048124034Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAAC188480.12896995037106573Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA186860.1278614437942346No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT186760.1277930174623315Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC184300.12610972969751388Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGCCTGGATACCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC183680.12568548643971433Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA183230.1253775679461501Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA182160.12464540619478637Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC180960.1238242902119485Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC179760.12300317422911065Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGA178240.1219630939841827Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC177400.1213883127961962Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC170880.11692691595611053Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGTGGGGTCTCCCCATGCGAGAGTAGATCGGAAGAGCACACGTCTGAA168590.11535995295552828Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAAC167640.11470990280244833Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC163700.11201390532546403Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATAACCTCGGGCCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC163570.11192495109398992Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA162760.11137069780557438Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC161460.11048115549083337No Hit
ACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT160770.11000901380070159Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCA159850.10937949154719258Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA159680.10926316678295722Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC159030.10881839562558672No Hit
CGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCC154520.10573236805675444Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC154480.10570499752399318No Hit
CCTCCCGGGGCTACGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAG149030.10197576243527126Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)