FastQCFastQC Report
Fri 29 Sept 2023
SRR11798523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11798523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30964994
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG24167357.804732660371257TruSeq Adapter, Index 8 (100% over 49bp)
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC4411501.424673293978355TruSeq Adapter, Index 5 (100% over 35bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3870161.2498500726336328Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCT2289770.7394705130574222Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC2168870.7004264234638637Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG2084460.6731666087195107Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG1921580.6205652744515306Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCAGATCGGAAGAGCACACGT1879780.6070661599353128No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTC1810350.5846440661348101No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC1587370.5126337179332249No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT1452190.4689779691221642No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCAGATCGGAAGAGCACACG1260640.4071177924336106No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA1249790.4036138356752144Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT1105610.35705157895396333Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1097060.3542903964392824No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG942430.30435336108897676No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG921050.2974487900756577Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAAC887710.28668179299501884Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGA878490.2837042371136904Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC864670.2792411327449313No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC847790.2737898156867074Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG824360.26622320676051153No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA776340.2507153723330287Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATAACCTCGGGCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA754500.24366224647096654Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG748350.2416761327323364Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC747290.24133381068958062No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC722110.23320204744751444No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC704340.22746330905150505No Hit
GGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT698120.22545458914024008TruSeq Adapter, Index 8 (100% over 37bp)
GCGACCTCAGATCAGACGTGGCGACCAGATCGGAAGAGCACACGTCTGAA691980.2234717048548435Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC663790.21436787618948028TruSeq Adapter, Index 8 (100% over 50bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC657770.21242374534288624Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT628240.202887169944228Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC623290.20128859059362325TruSeq Adapter, Index 8 (100% over 50bp)
GGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC609400.19680288005222932Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA606960.19601489346324433Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC589080.19024063108166597No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT553930.17888910296575544Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC551370.17806236293796796Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGAGATCGGAAGAGCACACGTCT520230.16800584556870898Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA512950.16565480361468823Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT498180.16088490118874235Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCG495260.15994190084454724TruSeq Adapter, Index 8 (100% over 50bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC494890.15982241107490605No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG493480.15936705816897623No Hit
CGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT468470.15129019563188031Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG468450.15128373672541323Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAGATCGGAAGAGCACACGTCTGAAC461740.14911677360570458Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA459240.14830941029731834Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGAGATCGGAAGAGCACACGTCTGAA456010.14726629690288331Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT441620.14261911369981212TruSeq Adapter, Index 8 (100% over 36bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA429970.13885680068273226No Hit
CCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAA424850.13720332062715723Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT416560.13452610389654845No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT413760.1336218569911559Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA408150.13181013372713718Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC403520.13031489688000586Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGT401960.12981110217557285No Hit
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA401590.1296916124059317Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA400860.1294558623198829Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAA394150.12728889920017422Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACCCAGATCGGAAGAGCACACGTCTGA393520.1270854436464609Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA389650.125835645245079Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCT382610.12356211016866336Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC380210.12278704139261258No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC376100.12145973611362561No Hit
AGTCCGCCCGGAGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA374660.12099469484799512Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG371360.11992897528092529No Hit
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT367910.11881481391535229Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTG365570.11805912185870278Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGAA363400.1173583305070235Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGA362900.11719685784534627Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG361190.11664462134241006No Hit
CCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA359000.11593737108426373Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT344360.11120945155035393No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAATAGATCGGAAGAGCACACGT344130.1111351741259824No Hit
GCTTTCGCCGAATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA337580.10901988225801044Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT326250.10536091174440403Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA322480.10414340787535756Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTTGGATAACTGTGGTAATTCTAGAGAGATCGGAAGAGCACACGTCTGA320410.10347491105601377Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGATCGGAAGAGCACACGTCTGA318160.10274828407846616Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC317480.10252868125858511Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA317230.10244794492774646No Hit
CGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC317090.10240273258247684TruSeq Adapter, Index 5 (100% over 35bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT315410.1018601844392413No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGATCGGAAGAGCACACGTCTG313180.10114001636816078Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)