FastQCFastQC Report
Fri 29 Sept 2023
SRR11798524_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11798524_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8792392
Total Bases448.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG6734657.659633464931955TruSeq Adapter, Index 8 (100% over 49bp)
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC1237901.4079217578106162TruSeq Adapter, Index 5 (100% over 35bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1088331.2378087783165264Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCT636990.7244786174228811Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC609440.6931447096535277Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG581830.6617425610687058Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG535790.6093791086657646Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCAGATCGGAAGAGCACACGT525170.5973004843278144No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTC504220.573473066260012No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC450120.5119425976457828No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT405900.46164911664539066No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCAGATCGGAAGAGCACACG355010.4037695316587341No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA351950.40028925006983307Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT309660.3521908486336824Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT309190.351656295579178No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG260060.2957784411796016No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG259680.2953462493483002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAAC248470.28259659032490814Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGA243720.27719419243364035Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC239660.27257656392026197No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC235400.2677314660219881Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG231870.26371663137858276No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA216170.24586028466428703Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC212820.24205017246728763No Hit
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG209170.23789885619294499Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATAACCTCGGGCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA208510.2371482072227899Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC201320.22897068283579713No Hit
GGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT197320.2244212951378874TruSeq Adapter, Index 8 (100% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC192200.21859807888456292No Hit
GCGACCTCAGATCAGACGTGGCGACCAGATCGGAAGAGCACACGTCTGAA191890.21824550133797493Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC185090.21051154225152835TruSeq Adapter, Index 8 (100% over 50bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC183160.20831646268728693Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT176590.2008440933934702Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC173340.1971477158889185TruSeq Adapter, Index 8 (100% over 50bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA170370.19376979552322054Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC169720.1930305200223102Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC166750.18965259965661221No Hit
AGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC153960.17510593249254583Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT153160.1741960549529639Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGAGATCGGAAGAGCACACGTCT144040.16382345100172968Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA142750.1623562734691538Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT140620.15993372452001686Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC140400.1596835081966318No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG138540.15756804291710377No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCG138170.15714722455504715TruSeq Adapter, Index 8 (100% over 50bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA130130.14800295528224855Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG129950.14779823283584262Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAGATCGGAAGAGCACACGTCTGAAC129630.14743428182000984Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT129270.14702483692719798Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCTTTCGCCGAATCCCGGGGCCGAGAGATCGGAAGAGCACACGTCTGAA128420.14605809204139214Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT123260.1401893819110886TruSeq Adapter, Index 8 (100% over 36bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA120660.13723227990744727No Hit
CCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAA119680.13611767992145937Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT118040.13425243096531636No Hit
GGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA113520.12911162286667838Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT113310.1288727800125381Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC112290.12771268614957113Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA111860.12722362697204584Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGT111460.12676868820225487No Hit
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA111380.12667770044829668Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGACCTCAGATCAGACGTGGCGACCCAGATCGGAAGAGCACACGTCTGA110310.1254607392391058Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA109000.12397081476804037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAA108290.1231632984516614Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC106550.12118431480307067No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCT106250.12084311072572743Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC104620.1189892352388292No Hit
AGTCCGCCCGGAGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA104060.11835232096112185Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT104060.11835232096112185Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG103820.11807935769924725No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG103670.11790875566057564No Hit
GACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGAA102150.11617998833536995Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTG101500.11544071283445961Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGA101030.1149061597799552Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA100700.11453083529487766Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT97570.11097093942126329No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAATAGATCGGAAGAGCACACGT96560.1098222190275411No Hit
GCTTTCGCCGAATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA95770.1089237149572039Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT93200.10600073336129691Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA90340.10274792115729144No Hit
ACTTGGATAACTGTGGTAATTCTAGAGAGATCGGAAGAGCACACGTCTGA89870.10221336810278706Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA89780.10211100687958408Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGATCGGAAGAGCACACGTCTGA89540.1018380436177095Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT89170.10141722525565285No Hit
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC88330.10046185383909179Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGATCGGAAGAGCACACGTCTG87970.10005240894627992Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)