FastQCFastQC Report
Fri 29 Sept 2023
SRR11798525_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11798525_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15638090
Total Bases797.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG11911807.617170639125366TruSeq Adapter, Index 8 (100% over 49bp)
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC2017561.2901575576045412TruSeq Adapter, Index 5 (100% over 35bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1745891.1164342960041795Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC1045390.6684895661810362Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCT1025100.6555148358910838Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG935490.5982124415449713Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG914490.584783691614513Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCAGATCGGAAGAGCACACGT880030.5627477524429134No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTC793380.5073381723727131No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC723130.46241580653391817No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT659400.42166274781638935No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA607150.3882507390608444Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCAGATCGGAAGAGCACACG591240.37807686232781623No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT505110.3229998036844653Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT499430.31936764656041755No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG429120.27440691286467844Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG427690.2734924789408425No Hit
CGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAAC413760.26458474148697186Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGA408980.2615281022170866Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC402850.2576081861659576Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC395480.2528953344046491No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG371650.23765690055499106No Hit
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG351910.22503387562036029Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATAACCTCGGGCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA350770.22430488633842113Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC346010.22126103635418393No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA344970.22059599350048503Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC331750.21214227568712038No Hit
GGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT324240.20733989892627552TruSeq Adapter, Index 8 (100% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC323900.20712248107025857No Hit
GCGACCTCAGATCAGACGTGGCGACCAGATCGGAAGAGCACACGTCTGAA320970.20524885072281845Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC297270.1900935472298727TruSeq Adapter, Index 8 (100% over 50bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC295900.1892174811629809Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC290000.18544464189680454Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC286650.18330243655075523TruSeq Adapter, Index 8 (100% over 50bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA286610.18327685797945914Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT286180.18300188833802594Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC270110.17272569731981335No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT257080.1643934777201052Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC256000.16370285629511022Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA241630.15451375455698235Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGAGATCGGAAGAGCACACGTCT239860.15338190277712943Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT232660.14877775994382947Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCG231320.14792087780540975TruSeq Adapter, Index 8 (100% over 50bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC222710.14241509033392186No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG222360.14219127783508087No Hit
CGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT218670.13983165463301464Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTTCGCCGAATCCCGGGGCCGAGGAGATCGGAAGAGCACACGTCTGAAC218400.1396589992767659Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG216340.13834170285501618Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA215300.1376766600013173Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGAGATCGGAAGAGCACACGTCTGAA214950.13745284750247633Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT203630.1302141118256769TruSeq Adapter, Index 8 (100% over 36bp)
CCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAA197920.12656277077315709Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA197060.12601283149029072No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT191240.12229114936670654No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT188350.12044309759056253Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC186270.11911301188316475Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA185980.11892756724126796Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA184460.11795558153201574Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGACCTCAGATCAGACGTGGCGACCCAGATCGGAAGAGCACACGTCTGA184150.11775734760447087Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAA184010.11766782260493448Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA183640.11743122082044546Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGT182840.11691964939452325No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCT179280.11464315654916937Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGAA174020.11127957442373078Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA171920.10993669943068496Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC171840.10988554228809272No Hit
ACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGA171270.10952104764712314Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT168960.10804388515477273Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG168330.10764102265685899No Hit
AGTCCGCCCGGAGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA167950.10739802622954595Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTG167820.10731489587283358Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA165550.10586331195177928Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC163560.10459077802979774No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAATAGATCGGAAGAGCACACGT162360.10382342089091443No Hit
GCTTTCGCCGAATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA159460.10196897447194639Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCCCGTATG157290.10058133697913235TruSeq Adapter, Index 8 (97% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT156940.1003575244802914No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)