FastQCFastQC Report
Fri 29 Sept 2023
SRR11798526_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11798526_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30715307
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG396304112.902495163079438TruSeq Adapter, Index 11 (100% over 49bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC4043071.3163046034343724Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTC2486600.8095637787374224No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCT1760900.5732972162707017Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC1680770.5472092465167286No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1553050.5056273733484089No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1328150.4324065522118988No Hit
AGATAACCTCGGGCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1210790.39419759014617695Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG1131430.3683603097309104Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1072960.3493241985176967Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG1070060.34838004386542515Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGTGGGGTCTCCCCATGCGAGAGTAGATCGGAAGAGCACACGTCTGAA1038730.33817991791519453Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT933300.30385501274657617No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG904240.29439393198967534Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC880730.28673976789488054Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTG864020.28129948367437774Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGA815750.26558419227260205Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT809930.26368937155666394Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT802770.2613582862772623Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGA797580.25966857502026597Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT756930.2464341313599763No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAA750040.24419095013440695Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA717430.23357409385489783Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC716200.23317364205410676Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCC700900.22819241233694976Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG684200.22275538382214444Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTTCGCCGAATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA682250.22212052121113424Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGA666180.21688860215527067Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC630480.20526573281523772Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCT610280.19868920730631145Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA604030.19665439124538134No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC592590.19292986392745481No Hit
CGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAAC582310.1895829984704369Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT566380.18439665929433816Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCAGATCGGAAGAGCACACG563180.18335483347114193No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG556200.18108235089429514Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC549360.17885544819721322TruSeq Adapter, Index 11 (100% over 50bp)
CCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA546400.17789175931075668Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT546390.1778885036050592No Hit
CGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTC545370.1775564216239154Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATAACCTCGGGCCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC533810.173792825837619Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT518480.16880182900336954Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT517570.16850555978489812Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA508170.1654451964292592Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG503520.16393129327992717Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCC498440.16227739478560316Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG486150.15827613248339012Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAGATCGGAAGAGCACACGTCTGA478580.15581156327039153Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG471340.15345443234541006TruSeq Adapter, Index 11 (100% over 35bp)
GAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCA456360.1485773852105727Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA453340.14759416208993126Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGT439990.14324779498378445No Hit
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC439790.1431826808698347Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC439450.1430719868761201Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG431900.1406139290745165Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGATCGGAAGAGCACACGTCTG431000.14032091556174256Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC428940.13965024018805997No Hit
AGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT423170.13777169800060926Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC421840.13733868914284333Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTCCCGGGGCTACGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAG411550.13398856798012795Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA409260.13324301137540315Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC407740.13274814410938493Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA407370.13262768299857788Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC392270.12771156739537065TruSeq Adapter, Index 11 (100% over 50bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTG389160.1266990429234518Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT383550.12487259202716092Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCTTTCGCCGAATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCC378860.12334566605503895Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA377900.12303311830808009Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG377870.1230233511909876No Hit
AGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC370030.12047087792415685Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA367930.11978717972768432Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAAGGTGTGCTGTGAGGCATGCTGGAGGTAGATCGGAAGAGCACACGTCT366670.11937696080980079Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT361350.11764492537873707TruSeq Adapter, Index 11 (100% over 37bp)
AACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA357590.11642078003648149Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACT355380.11570126907733658Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC354960.11556452943804207Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC352080.11462688619716546Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCGGTGCGCCGCGACCGGCTCCAGATCGGAAGAGCACACGTCTGAACTC349030.11363389595943156Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT343180.11172930812640094Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACTCC343110.11170651818651853Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG343090.11170000677512355Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGTC340600.11088933605644899No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG328710.10701830198213548No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGAGATCGGAAGAGCACACGTCTGAA328320.10689132945993345Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGACGGCCGGGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC323670.10537742631060142Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC321180.10456675559192684Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGACGGCCGGGAAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGT313110.10193940109405385Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAGATCGGAAGAGCACACGTCTGAA313000.10190358833138148Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC308510.10044177647320926No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)