FastQCFastQC Report
Fri 29 Sept 2023
SRR11798527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11798527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33347594
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG433256412.992133705358174TruSeq Adapter, Index 11 (100% over 49bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC4419021.3251390789992226Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTC2746960.8237355894401258No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCT1929430.5785814712749592Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC1885110.5652911571371536No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1729570.5186491115371022No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1497680.44911186096364253No Hit
AGATAACCTCGGGCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1400860.420078282109348Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG1239370.3716519998414278Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1229600.36872225324561647Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG1194590.35822374471753493Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGTGGGGTCTCCCCATGCGAGAGTAGATCGGAAGAGCACACGTCTGAA1135930.3406332702743113Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1018680.3054733124074859No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG998860.2995298551373751Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC973440.2919071162975056Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTG971450.29131037159682344Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT916700.27489239553534206Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT909580.27275730896807726Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGA905250.271458864468603Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGA886730.2659052404200435Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT882600.26466677026234636No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAA824530.2472532201273651Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCC821120.24623065760006552Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC818520.24545099115696323Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA802040.24050910539453013Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG755970.2266940157661749Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTTCGCCGAATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA753560.22597132494776082Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC742280.22258877207153235Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGA735320.22050166497768925Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA696720.20892661701470877No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCT691850.20746624179243636Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAAC683580.20498630275995322Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA651020.19522247991864122Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC648840.1945687595932708No Hit
CGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTC644240.1931893497323975Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT632530.18967785202134824Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT631720.18943495593715096No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG628840.1885713254155607Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCAGATCGGAAGAGCACACG617070.18504183540197833No Hit
AGATAACCTCGGGCCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC602720.18073867637947133Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC601970.1805137725978072TruSeq Adapter, Index 11 (100% over 50bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT579650.17382063605548276Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA578830.1735747412541966Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT575040.17243822747752055Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCC573020.17183248662557185Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG559550.1677932147068841Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG550420.16505538600475944Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG545550.16359501078248703TruSeq Adapter, Index 11 (100% over 35bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAGATCGGAAGAGCACACGTCTGA536100.160761223133519Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCA520940.15621516802681476Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC509670.15283561386767514Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA505410.15155816038782288Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGT501960.15052360299216788No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC487100.1460675093981293No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGATCGGAAGAGCACACGTCTG483870.14509892377842912Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC480210.14400139332390816Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG479340.14374050493717777Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT467620.14022600850903966Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC465970.13973122018937856Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA455790.13667852619292414Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTCCCGGGGCTACGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAG453130.1358808674472887Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC452050.13555700600169235Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA448210.13440549863957202Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTG443540.1330050977590767Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG428500.12849502725743872No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC428450.12848003367199445TruSeq Adapter, Index 11 (100% over 50bp)
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA423670.12704664690352174Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT421600.12642591246612875Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCTTTCGCCGAATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCC420750.12617102151357606Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC419900.12591613056102338Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACT415690.12465367066661541Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA415230.12451572968052808Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC414420.12427283359633082Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAGGTGTGCTGTGAGGCATGCTGGAGGTAGATCGGAAGAGCACACGTCT407740.12226969058097566Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC402830.12079732049034783Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA398300.11943890164909647Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT397670.11924998247249861TruSeq Adapter, Index 11 (100% over 37bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT390020.1169559638995245Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGTGCGCCGCGACCGGCTCCAGATCGGAAGAGCACACGTCTGAACTC388100.11638021021846434Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACTCC387160.11609833081211195Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGTC380750.11417615315815588No Hit
AGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG379580.11382530325875984Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTTTCGCCGAATCCCGGGGCCGAGAGATCGGAAGAGCACACGTCTGAA370390.11106948225410206Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG369670.11085357462370449No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC362160.10860153808997434No Hit
GGGACGGCCGGGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC360180.10800779210638105Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC356550.10691925780312665Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAGATCGGAAGAGCACACGTCTGAA346510.1039085458459162Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGACGGCCGGGAAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGT345360.10356369338069787Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA344550.10332079729650061No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC343640.10304791404141479No Hit
CGACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC337960.10134464273494513Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCG336100.1007868813564181TruSeq Adapter, Index 11 (100% over 50bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG335170.10050800066715457No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)