FastQCFastQC Report
Fri 29 Sept 2023
SRR11798528_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11798528_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19529567
Total Bases996 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG251565212.88124821200593TruSeq Adapter, Index 11 (100% over 49bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC2594501.3284984761822933Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTC1603850.8212419660917214No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCT1125720.5764183097351825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC1102220.5643852728532076No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1018780.5216603112603572No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC884180.452739172353386No Hit
AGATAACCTCGGGCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA808670.41407472065304884Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG727780.3726554715729232Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG723400.37041271831577216Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG700020.35844112672851375Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGTGGGGTCTCCCCATGCGAGAGTAGATCGGAAGAGCACACGTCTGAA668630.3423680617189311Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT598520.30646864828083487No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG579320.2966374011262001Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTG566120.2898784187073887Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC565260.28943806076192063Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT535490.2741945072310103Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT534150.2735083681066764Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGA525030.26883852570822486Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT513240.26280152550233193No Hit
GACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGA512760.26255574432346607Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAA479730.24564292695275833Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCC479440.24549443415719355Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC474460.24294445442646015Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA465850.2385357545305536Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTTCGCCGAATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA443280.22697891868263131Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG437560.22405002630114637Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC436120.22331268276454874Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGA424520.21737297094195687Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA405230.20749563981628472No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCT403430.20657396039553771Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAAC397950.20376795860348568Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA378020.19356291923932567Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC376820.192948466292161No Hit
CGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTC373120.19105390303840325Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT372610.19079276053585828No Hit
CGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT367950.18840663492436877Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG366800.18781778418333597Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCAGATCGGAAGAGCACACG359540.18410034385298968No Hit
AGATAACCTCGGGCCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC351320.17989134116491162Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC349250.17883140983105258TruSeq Adapter, Index 11 (100% over 50bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA346590.17746937246483754Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCC337760.17294802286195082Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT337540.1728353731549706Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT337190.1726561577120476Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG327630.16776101589963566Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG320520.16412038218768496TruSeq Adapter, Index 11 (100% over 35bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG316240.16192883334279762Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAGATCGGAAGAGCACACGTCTGA316220.16191859246034487Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCA306750.1570695346189703Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA296930.1520412613346727Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC293500.150284949994027Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGT288290.14761720011508703No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGATCGGAAGAGCACACGTCTG283370.14509794303171186Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC283120.14496993200105254No Hit
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC282480.14464222376256475Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG278770.1427425400675806Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT272310.1394347350353441Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC269180.13783203693148957Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC268380.1374224016333798Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA265600.13599891897244828Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTCCCGGGGCTACGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAG262910.13462152028255414Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA262830.13458055675274316Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTG259410.13282936585332383Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC251500.12877909684326333TruSeq Adapter, Index 11 (100% over 50bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG251290.12867156757750953No Hit
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA247140.12654658446856504Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT245390.12565050725394988Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC244850.12537400342772576Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACT243990.12493364548225776Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTTTCGCCGAATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCC243110.12448304665433699Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA243100.12447792621311062Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC242260.12404780915009535Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT235200.1204327776442765TruSeq Adapter, Index 11 (100% over 37bp)
CGGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC234930.12029452573116443Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA234010.11982344513833819Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAAGGTGTGCTGTGAGGCATGCTGGAGGTAGATCGGAAGAGCACACGTCT233490.11955718219456683Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT227810.11664877157798736Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGTGCGCCGCGACCGGCTCCAGATCGGAAGAGCACACGTCTGAACTC225400.11541474524243164Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACTCC224880.11514848229866029Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGTC222760.11406294875866935No Hit
AGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG219130.11220422859349621Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTTTCGCCGAATCCCGGGGCCGAGAGATCGGAAGAGCACACGTCTGAA216520.11086779343341305Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG215540.11036599019322854No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC212890.10900907326823989No Hit
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC208510.10676632001108884Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGACGGCCGGGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC208460.10674071780495697Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA204490.10470790263808717No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAGATCGGAAGAGCACACGTCTGAA202870.10387839115941484Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC201580.10321785424121281Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGACGGCCGGGAAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGT201440.10314616806404361Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCG198550.10166636054962201TruSeq Adapter, Index 11 (100% over 50bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC198000.10138473628217154No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGATCGGAAGAGCACACGTCTGA196820.1007805242174596Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG196280.10050402039123552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)