FastQCFastQC Report
Fri 29 Sept 2023
SRR11798529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11798529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16688159
Total Bases851 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG210908712.638224503973147TruSeq Adapter, Index 11 (100% over 49bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC2103971.2607562044441212Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTC1193120.7149500433211357No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCT860810.5158208284089335Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC855590.5126928620466763No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT786990.4715858711557099No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC684760.41032686709181043No Hit
AGATAACCTCGGGCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA633260.3794666625599624Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG565650.3389529066687344Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG563260.3375207534875477Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG546370.32739980485564646Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGTGGGGTCTCCCCATGCGAGAGTAGATCGGAAGAGCACACGTCTGAA514450.3082724703186253Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG451450.270521152153452Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC451200.27037134533533624Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT446970.2678366139728175No Hit
GACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTG439610.2634263012474893Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT416840.24978189625350528Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT416400.2495182362536215Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGA414770.24854149579950668Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGA404770.24254922307487603Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT396630.23767151307702664No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA381380.22853329717196486Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCC376880.22583677444588104Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAA376490.22560307580962047Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC369100.2211747862661184Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG354020.21213843899737533Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTTCGCCGAATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA349340.20933405536224817Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGA328420.1967982208223208Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC323190.19366426218733893Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCT317040.18997901446169108Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA313070.1876000821900127No Hit
CGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAAC307990.1845560076459003Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTC295930.1773293267399957Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA292180.17508222446825922Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG289530.17349427219623206Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCAGATCGGAAGAGCACACG289220.17330851174176853No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT286830.17187635856058178No Hit
CGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT286820.17187036628785715Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCCCGTATG286640.1717625053788138TruSeq Adapter, Index 11 (97% over 49bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC285200.170899618106467No Hit
AGATAACCTCGGGCCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC274120.1642601799275762Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT268150.16068279311097167Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC264410.1584416831119598TruSeq Adapter, Index 11 (100% over 50bp)
CGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCC264000.15819599993024994Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA263010.1576027649305115Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT262240.15714135993071496Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAGATCGGAAGAGCACACGTCTGA253460.15188014447848922Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG251160.15050192175182414Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG250070.14984876402483943TruSeq Adapter, Index 11 (100% over 35bp)
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC243990.14620546220826394Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCA241920.1449650617542654Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG239980.14380256084568704Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA230510.1381278785754618Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC229530.137540635848448Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGT226900.1359646681218701No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGATCGGAAGAGCACACGTCTG225530.1351437267585957Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC222190.13314230766856908No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG213870.12815673676167635Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA213410.12788109221634333Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT212690.1274496485801699Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC210960.12641298539880882Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA208220.12477110267226002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC206460.12371646267272501Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTG205610.1232071194911314Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG197050.11807773403884755No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC196080.11749648358455836TruSeq Adapter, Index 11 (100% over 50bp)
CCTCCCGGGGCTACGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAG195770.1173107231300948Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTTTCGCCGAATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCC194030.11626806767600908Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA191650.11484190676754698Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC190780.1143205790405041Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAGGTGTGCTGTGAGGCATGCTGGAGGTAGATCGGAAGAGCACACGTCT190340.11405691904062035Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA189220.11338578449546172Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACT188670.11305620949560705Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC187460.11233114449592672Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT185550.11118662040552225TruSeq Adapter, Index 11 (100% over 37bp)
CGGGACGGCCGGGAAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC185080.11090498358746462Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT183530.10997618131514686Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT182540.10938294631540843Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA180960.10843616722491678Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCGGTGCGCCGCGACCGGCTCCAGATCGGAAGAGCACACGTCTGAACTC176420.10571567540793446Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACTCC174020.10427752995402309Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCAGATCGGAAGAGCACACGTC173860.104181653590429No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGAGATCGGAAGAGCACACGTCTGAA172850.1035764340452413Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG167150.1001608385922018No Hit
AGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG166900.10001103177408606Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)