FastQCFastQC Report
Fri 29 Sept 2023
SRR11798531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11798531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29035992
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG19166786.601041906885771TruSeq Adapter, Index 12 (100% over 49bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA3756781.2938355954912786No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG3590741.2366513945864153Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT3560131.226109305995125No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC3502811.2063682893975174Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC2924811.0073050027014747No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2797000.96328721953085No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA2610480.8990497035541269No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA2376040.818308532389732Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT2360570.8129806620693381No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC2067890.7121816261693418No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2034520.7006889931640703No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC1782100.6137555073027986No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG1732710.5967455838946367No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1605260.5528517847780092No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC1509970.5200338944851618No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT1484190.5111552586183382No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT1419040.4887175888462843Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC1403490.48336216651389075Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1364520.4699408926686576No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC1272130.4381217628107901No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1265630.4358831618358347Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG1247770.42973217515695694Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1247280.4295634190834603No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA1245840.42906748286747015Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG1208310.41614214523822707No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC1159320.3992699818900625No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1057340.36414805459376076No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1037440.357294491608897No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT1033480.3559306670149241Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAAC1032330.355534606842432Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT1005750.346380450855614Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA965150.3323978047658919Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC951300.32762786268848676No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT950960.32751076663748907Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA934630.3218867121881009No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC837840.28855222167026356No Hit
CCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAA825380.2842609958013489Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA772080.26590446780671384Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG737820.2541053186679484Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC701340.24154160119619814TruSeq Adapter, Index 12 (100% over 50bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA699470.24089757291571096No Hit
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGC688770.2372124913107842No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG683440.23537683851132069TruSeq Adapter, Index 12 (100% over 50bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA662170.22805144732096633No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT657360.2263948825994993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA653720.2251412660535242Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG634620.21856322318865498Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGAA621350.2139930331982458Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG576100.19840892641105565Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCT573410.19748249000757406Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC553310.19056004699271167Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT551780.19003311476322216No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGC549700.18931676245123638No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA530510.18270772357286777Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC527590.18170207513488776No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC509390.17543399240501237TruSeq Adapter, Index 12 (100% over 50bp)
GCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACT500540.17238605107757296Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG496520.1710015624746005No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCA489380.16854254540364938No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG481090.16568746816020613No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC464530.15998420167631952No Hit
GACGTACGCAGTTTTATCCGGTAAAAGATCGGAAGAGCACACGTCTGAAC456210.15711879242837648Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA440410.15167727005848466Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA436060.15017912940601444No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC434040.14948344110302828No Hit
TGGCGGTGCGCCGCGACCGGCTCCAGATCGGAAGAGCACACGTCTGAACT428190.14746870022556832Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC412270.1419858498376773Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC409320.1409698693951975No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACG407900.14048082118220726No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC406110.1398643449137195No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT401550.1382938802297507No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG384900.13255961773236472No Hit
AGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAACTCCA379600.13073429693740102Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCA379160.13058276087140402No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT373410.12860246000894338No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC366260.12613999893649233Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT365650.12592991484499652No Hit
AGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCA362550.12486227438001773Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCAC360130.12402882601703431No Hit
GGCGGTGCGCCGCGACCGGCTCCAGATCGGAAGAGCACACGTCTGAACTC351270.12097744068809498Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC350350.12066059255010128Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAGAGCACACG348910.12016465633411112No Hit
GCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGA341990.1177814072961585Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG333640.11490566604371566No Hit
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC333190.11475068597621876Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCAC331260.11408599368673197No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT330860.11394823362673472Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC329230.11338686138224587Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG316010.10883389139933637No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG315470.10864791531834009No Hit
CGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTG312130.10749761881736294Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAG308240.10615790223388957No Hit
CCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACGTCTGA307180.10579283807489684Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAATGAGATCGGAAGAGCACACG302420.10415349336092943No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA299580.10317539693494887Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTTCGCCGAATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA297780.1025554766649612Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTTTCGCCGAATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCC296190.10200788042647208Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCAC295810.10187700836947469No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC295440.10174958031397721No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)