FastQCFastQC Report
Sun 1 Oct 2023
SRR11836448_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11836448_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145591529
Total Bases7.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG135479579.305456912949928TruSeq Adapter, Index 4 (100% over 49bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA59648474.096973938641719No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC48228103.3125622301830484No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA27196341.8679891740130017No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC15170831.04201323416282No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC14859311.020616384899701No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT14030550.9636927434150375No Hit
GCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAG11406550.7834624774082838Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAGAAAGCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG11084870.7613677853469071TruSeq Adapter, Index 4 (100% over 35bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC11080140.7610429038079544No Hit
GGGAGAAAGCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA11010580.7562651533112205TruSeq Adapter, Index 4 (100% over 36bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA10817800.7430239983261664Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA8293440.5696375370850044Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG7306090.5018210915279281Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATACAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA6702320.46035095901767753TruSeq Adapter, Index 4 (100% over 39bp)
GCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGT6532420.44868132403499933Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC6156520.4228625142057543No Hit
GGCCCGAGGTGGGATCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGT6117800.42020301881711813Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAATCAAACTCCGAATACCATTAAGATCGGAAGAGCACACGTCTGAACTC5978680.4106475178236503Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA5946870.4084626379602071No Hit
AGAGCCACCTTACAAATAAGCGCTCTCAGATCGGAAGAGCACACGTCTGA5201860.3572913915891357Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAACTCC5069470.34819814276419886Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGAGCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC4996710.3432005992601397TruSeq Adapter, Index 4 (100% over 38bp)
CACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT4993260.3429636349241171Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCCGAGGTGGGATCCCGAGGCCAGATCGGAAGAGCACACGTCTGAACTC4716650.3239645900002877Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT4483210.3079306901159064Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG4480980.30777752186392654No Hit
GGCCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAACTC4422420.3037553098298734Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT4110230.28231244140584577Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC4040950.27755392279725283Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAAGTAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA3942080.2707630057240487TruSeq Adapter, Index 4 (100% over 39bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG3866790.2655916883735729No Hit
ATTGATCATCGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA3834130.2633484259925589TruSeq Adapter, Index 4 (100% over 36bp)
CGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCTGAACTCC3570470.2452388558952492Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGTAAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC3513860.24135058022503492TruSeq Adapter, Index 4 (100% over 38bp)
TTTCGCCGAATCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG3471520.23844244399686193TruSeq Adapter, Index 4 (100% over 35bp)
TTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGAA3330140.2287317141919706Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC3252190.22337769390415566No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT3178920.21834512088955396No Hit
GCCCGAGGTGGGATCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC3096910.212712238223695Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC2959130.20324877555204465No Hit
ATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAACTC2956890.20309492044691693Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2920750.2006126331704367No Hit
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC2915910.20028019624685722Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG2878640.19772029456466522Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAG2856120.19617350127561334No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT2828820.1942983921818693No Hit
CACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC2801510.19242259623497737Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTC2751290.18897321972626582Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC2750930.18894849301294173Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC2740380.18822386294191606Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA2707080.1859366419594371Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATG2674990.18373253020785296TruSeq Adapter, Index 4 (97% over 49bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA2644950.1816692233515866Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGTGACTCTAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2631660.18075639551803868Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC2622170.18010457188068957Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA2539040.17439476166226678No Hit
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC2507070.17219889214845735Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2463930.16923580766845303No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG2443220.16781333479916954Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAGAGCGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC2359290.1620485763289154TruSeq Adapter, Index 4 (100% over 37bp)
GGACGTACCGCTGGTGTTCCAGTTAGATCGGAAGAGCACACGTCTGAACT2354330.16170789716756118Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACGTACCGCTGGTGTTCCAGTTAGATCGGAAGAGCACACGTCTGAACTC2305350.15834369044918814Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA2264300.15552415827709318No Hit
AATCAAACTCCGAATACCATTAAGATCGGAAGAGCACACGTCTGAACTCC2190730.15047097966805473Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTTCGCCGAATCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2177660.14957326260376042Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAGAGATCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC2172680.1492312097361104TruSeq Adapter, Index 4 (100% over 38bp)
GAATACAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA2150810.14772906190167148TruSeq Adapter, Index 4 (100% over 40bp)
ACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2047560.1406373031496908Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACT2036990.1399112993723694Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCTGGATACCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2025800.13914271069987869Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA1952900.13413555125174897Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1915920.13159556831084587No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1914640.13150765110791576Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCCGGTGCGGAGAGCCGTTAGATCGGAAGAGCACACGTCTGAACTCCAG1874620.12875886481005364Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAACT1858250.12763448620695508Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAACT1840240.12639746368760232Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1808040.12418579655139139Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGACGTACCGCTGGTGTTCCAGTTAGATCGGAAGAGCACACGTCTGAAC1789700.1229261078781582Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGGTGACTCTAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1779300.12221178060435095Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAGAGCGACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG1772980.12177768941488347TruSeq Adapter, Index 4 (100% over 35bp)
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA1767740.12141777836538828Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1761800.12100978759554068No Hit
TTCCTCAAAGATGAGTATTCCTAGATCGGAAGAGCACACGTCTGAACTCC1742140.1196594343067858Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATCAAACTCCGAATACCATTAAAGATCGGAAGAGCACACGTCTGAACT1629820.11194469974966743Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAGAAAGCCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1590350.10923369037493932Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGGTGCGGAGAGCCGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1583460.10876044855604203Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAGCTGACCAATACTAATATAGATCGGAAGAGCACACGTCTGAACTCCAG1576960.1083139940099125Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC1563210.10736957093156155No Hit
AACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT1543060.10598556183855998Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGAGAGCGACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1511610.10382540868844094Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGCCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAACT1506650.1034847295270867Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1473730.10122360896422757TruSeq Adapter, Index 4 (100% over 50bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC1467970.10082798155104201No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)