FastQCFastQC Report
Fri 29 Sept 2023
SRR11836450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11836450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140445387
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG3604251525.663010918258212TruSeq Adapter, Index 12 (100% over 49bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA24067541.7136582777190112No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG18116281.2899163430693525TruSeq Adapter, Index 3 (100% over 49bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC17574611.2513483265918872No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA12692750.9037498682673003No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC8922920.6353302298209339No Hit
GCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAG8412330.5989751731753211Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC8096620.5764959727726764No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG6907830.49185168324538847No Hit
GGGAGAAAGCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT5860220.41725969967244275TruSeq Adapter, Index 12 (100% over 35bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA5524850.393380666892249Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGCCACCTTACAAATAAGCGCTCTCAGATCGGAAGAGCACACGTCTGA5283720.3762117156614051Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGT5014740.3570597872324564Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG4598560.32742691648533817Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGAAAGCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT4469730.31825395589532607TruSeq Adapter, Index 12 (100% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATG4440850.31619764058181565TruSeq Adapter, Index 12 (97% over 49bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC4009360.2854746663911432No Hit
CACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT3915640.27880160990976516Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG3900900.27775209163687237No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT3629880.2584549110181882Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGAGTAGGGCGGGGCACGTGAAACCCTGTCTGAATAGATCGGAAGAGCA3535590.2517412693661487No Hit
TACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGAGATCGGAAGAGC3400240.24210407138541332No Hit
GGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC3301110.235045811793021TruSeq Adapter, Index 12 (100% over 44bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG3223560.22952409252145817TruSeq Adapter, Index 12 (100% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC3041180.21653826195088913TruSeq Adapter, Index 12 (100% over 50bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3009400.21427546068138215No Hit
GGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCG2951350.21014218145876162TruSeq Adapter, Index 12 (100% over 45bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC2832620.2016883616120478Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA2658350.1892799796977312No Hit
GATCACCTGATACCATTCCGAACTCAGAAGATCGGAAGAGCACACGTCTG2542660.1810426140945448Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC2534980.18049578232142294TruSeq Adapter, Index 12 (100% over 44bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT2514700.17905180467052292No Hit
GGCCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAACTC2355820.16773922236406383Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT2323660.16544936431411592TruSeq Adapter, Index 12 (100% over 43bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG2279630.162314337885658Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC2169420.15446715953725132Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGTGGACGTACCGCTGGTGTTCCAGTTAGATCGGAAGAGCACACGTCTG1938250.13800738076217484Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA1912560.1361782000002606Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1741770.12401760123313982No Hit
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGT1735610.12357899658178165TruSeq Adapter, Index 12 (100% over 46bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1731940.12331768504436533No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGAGATCGGAAGAGCACAC1709730.12173628742964694No Hit
GCCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAACTCC1701010.12111540552058146Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1676860.11939587592150677Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1626920.11584004535513866TruSeq Adapter, Index 12 (100% over 43bp)
TTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGAA1551750.11048778697160058Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1525340.10860734073095617No Hit
CTCTAGCATTACAAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT1518820.10814310334023287Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA1488650.10599493737733087Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1454590.10356979542517832TruSeq Adapter, Index 12 (97% over 43bp)
GTGGACGTACCGCTGGTGTTCCAGTTAGATCGGAAGAGCACACGTCTGAA1429000.10174773486864327Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)