FastQCFastQC Report
Fri 29 Sept 2023
SRR11836452_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11836452_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105289031
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG1332026412.651141219069629TruSeq Adapter, Index 5 (100% over 49bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC60346405.731499229012754No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA50714184.816663190679379No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA37735483.583989675049816No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC16102861.529395782928233No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC12550881.1920406029760118No Hit
GCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAG10139760.9630404899442944Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC8466940.8041616414914105Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA6400290.6078781368972804No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC6380510.6059994986562276No Hit
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC6324250.6006561120312713Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACT6023560.572097581560989Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC5780110.5489755148378181No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG5748580.5459809008974543Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT5393140.5122223985516592No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG5240700.49774415722374726No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG5105200.4848748204359483No Hit
CACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT4509390.4282867794651847Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCC4386160.41658280623743227Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGT4336840.411898557599984Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAACTCC3739130.35513006098422545Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT3441910.3269010995076971No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA3417420.3245751212203672No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA3394210.3223707130517708No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG3357890.3189211609327091Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG3134950.29774706540893137No Hit
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA3052740.28993903457996495Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT2992180.2841872483373885Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCCACCTTACAAATAAGCGCTCTCAGATCGGAAGAGCACACGTCTGA2953910.28055249174056884Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA2865570.27216225401485555Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT2625690.24937925395096475No Hit
TTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAA2467100.23431690619320067Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATG2458340.23348491069311866TruSeq Adapter, Index 5 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG2458150.23346686512861914TruSeq Adapter, Index 2 (100% over 49bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA2263460.21497586011595074Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGA2157490.20491118395799465No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAG2148360.20404404709546622No Hit
GGCCCGAGGTGGGATCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGT2068780.1964858048698349Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1933300.18361836761514122Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC1916550.1820275086395277No Hit
GGCCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAACTC1861670.17681518979883099Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGAGTAGGGCGGGGCACGTGAAACCCTGTCTGAATAGATCGGAAGAGCA1847300.1754503752627375No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1828010.17361827558276227No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG1655720.15725474764792927Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1617960.15366842914529244Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC1600470.15200728744478614No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1599250.15189141592536834No Hit
GCCCGAGGTGGGATCCCGAGGCCAGATCGGAAGAGCACACGTCTGAACTC1534130.145706536134804Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1529470.14526394492129005Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAATCAAACTCCGAATACCATTAAGATCGGAAGAGCACACGTCTGAACTC1501360.14259415114191715Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1480440.1406072395138673No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACG1463410.13898978707478085No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1426480.13548229919601026No Hit
GAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAAC1423030.13515462973536152Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1367550.1298853249015085No Hit
TTCTAGAGCTAATACATGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1348670.1280921656501901Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1281330.12169643768494745Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA1204020.11435379246675755Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAACT1145100.10875776793880837Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)