FastQCFastQC Report
Sat 30 Sept 2023
SRR11836454_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11836454_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220484500
Total Bases11 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG5552569825.18349271717513TruSeq Adapter, Index 6 (100% over 49bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA34385951.5595631438944688No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC30764211.3953003499112182No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA28510501.293084094346768No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC19360710.8780984604359945No Hit
GAATACAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA15857010.719189330769283TruSeq Adapter, Index 6 (100% over 40bp)
GGGAGAAAGCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC12918130.5858974213606852TruSeq Adapter, Index 6 (100% over 35bp)
GAACGAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA11242510.5099002424206691TruSeq Adapter, Index 6 (100% over 40bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC7163970.32491943878141094No Hit
AGAGCCACCTTACAAATAAGCGCTCTCAGATCGGAAGAGCACACGTCTGA5283160.23961593672117543Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG5042360.2286945340828947No Hit
GCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAG4467370.20261605691103002Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC4196970.1903521562740238No Hit
GCCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAACTCC4149440.1881964491835027Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAACGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA4144080.1879533481945443TruSeq Adapter, Index 6 (100% over 40bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG4100520.18597769911263604No Hit
GGCCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAACTC3660500.16602074068698705Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3443960.1561996421517159No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG3261200.1479106241028281Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA3159450.14329578723220907No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG2993150.13575330692180176TruSeq Adapter, Index 1 (100% over 49bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA2854400.1294603475527758Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATATCGTATG2808950.12739897815946247TruSeq Adapter, Index 6 (97% over 49bp)
GCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGT2636730.11958799824931005Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCCCGAGGTGGGATCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGT2608730.11831806770997509Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2575740.11682181740666578No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT2451670.11119466447754832No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT2404740.10906617018429866Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC2230120.1011463390850604Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)