FastQCFastQC Report
Tue 11 Jul 2023
SRR11869322_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11869322_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13609634
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG8915666.55099174599405Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC4819413.5411753174258767No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG2922102.1470819861871377No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2396071.7605690204453697Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1501981.1036152772366987Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGAGATCGGAAGAGCACACGT1371371.0076464951224993No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1324280.9730460054987518Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1271000.9338972671858773Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC1068560.7851496961637616No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1040400.7644584711095096Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG931170.684199148926415No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA907830.6670495327060229Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA849640.6242930559337598Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC814520.5984878065053035No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG779170.5725135591449411No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT707770.5200507228923277No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG704900.5179419226115853No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC704340.5175304493860746No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG702500.5161784659308252No Hit
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTTTAGATCGGAAGAGCACACG629680.4626722511420954No Hit
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT620010.45556699026586606No Hit
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA615300.4521062065298743Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG557540.40966568241291423No Hit
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT540440.3971010535624984No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA525300.38597658100137006No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG494450.3633088149174327Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA487980.3585548296155503Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCGGCCGTGCCGGTGGTCCCGGCGGATCTTTAGATCGGAAGAGCACAC457550.336195668450746No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC449920.3305893457531628No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTCTGA414540.3045930551842908Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT387330.284599865066173No Hit
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTC377830.2776195157048309No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG375720.27606914337299593No Hit
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTTAGATCGGAAGAGCACACGT371410.27290226908379756No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT363260.266913864105383No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG361610.2657014876373604No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT335340.24639898471920701Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGAGATCGGAAGAGCACACGTC316260.2323795041071641No Hit
TTCCGGCCGTGCCGGTGGTCCCGGCGGATCTTAGATCGGAAGAGCACACG271140.19922651850887396No Hit
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC265040.1947443994452753Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG250910.18436204823730012No Hit
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTAGATCGGAAGAGCACACGTC237110.17422217232292947No Hit
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG236950.1741046085442121Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCGCTGCGATCTATTGAAAGTCAGCCCTCAGATCGGAAGAGCACACGTC219040.1609448130640398No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC218760.16073907645128443Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGAATCTCGTATG212540.1561687845536478TruSeq Adapter, Index 6 (97% over 36bp)
TTGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT203890.14981299276674154Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT196140.14411849723512035Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGT190890.1402609357459576No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA186040.13669728370358822Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA179980.1322445555846689No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC176660.1298051071762841No Hit
TTCCGGCCGTGCCGGTGGTCCCGGCGGATCTAGATCGGAAGAGCACACGT175900.12924667922737673No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCT174870.12848986240188384Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA172980.12710114026578526No Hit
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT171450.12597693663180068No Hit
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC163990.12049552544910465Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC161140.11840142064070201Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGCATTTATCAGATCAAAACCAACCCGAGATCGGAAGAGCACACGTCTG160780.118136902138588Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA158720.11662326848760224No Hit
ATTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT156520.1150067665302388Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGAGTGCCGTTCCGAAGGGACGGGCGATGGAGATCGGAAGAGCACACGT152780.11225871320272095No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAGATCGGAAGAGCACACGTCTG151590.11138433259851074Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCCCAGATCGGAAGAGCACACGTCTG150390.11050260425813069Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC149060.10952535534754279No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT148650.10922409816457959No Hit
TCCGGCCGTGCCGGTGGTCCCGGCGGATCAGATCGGAAGAGCACACGTCT147840.10862893153482304Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCGTGCATTTATCAGATCAAAACCAACCCAGATCGGAAGAGCACACGT145320.10677730202002494No Hit
ATAACCTAGACTTACAAGTCAAAGTAAGATCGGAAGAGCACACGTCTGAA144200.10595435556900355Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT143310.10530040704988834Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG142620.1047934132541698No Hit
TTAACCTAGACTTACAAGTCAAAGTAAGATCGGAAGAGCACACGTCTGAA140360.10313282487978735Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC139930.10281687222448452No Hit
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCAGATCGGAAGAGCACAC138160.10151632292242393No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)