FastQCFastQC Report
Tue 11 Jul 2023
SRR11869326.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11869326.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53566918
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG30172365.632648120618028Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA10972172.0483108623124444Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA10403221.9420979194658912Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT9030461.6858278088726328Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG7011801.3089795459204876Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG6870241.2825527875245688No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC6610841.2341273769007954No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA5254810.9809804626056702Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA4703250.8780139264312351Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA4300160.802764123931864Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG4031430.7525969666576674Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC3988280.7445416217524405Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG3903130.7286456166845365No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG3444030.6429397338110809No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT3242160.6052541607863271No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT2981110.5565207242275914No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC2913860.5439663338480665No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG2640510.49293670395597516No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGAGATCGGAAGAGCACACGT2070910.38660241755928537No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC2069240.38629065797662654Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC1915630.35761437684355857No Hit
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA1859810.34719376612259084Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1756780.32795988001400417No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTCTGA1696340.3166767966751419Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT1671580.31205454082685885No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG1596420.29802349278336304Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTC1559350.2911031767778762No Hit
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT1554740.29024257098383No Hit
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA1485510.2773185494823503TruSeq Adapter, Index 7 (100% over 35bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC1455590.27173301252836685No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATG1400750.26149535054452827TruSeq Adapter, Index 9 (97% over 36bp)
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1384700.2584990982680766Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1344090.25091792662030693No Hit
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT1327230.24777046161214653Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGT1248860.23314016311336036No Hit
CTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1217510.22728767034907627Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG1211190.22610783767697817No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC1197570.2235652235956528Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1165190.21752044797499828Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1158080.21619313621888794No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA1006680.18792942315628464Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG973860.1818025072863068No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT951810.17768616070090126Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT944670.17635324847324613Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG851010.1588685763104758Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTTTAGATCGGAAGAGCACACG759700.14182260775204575No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA717860.1340118167709406Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCGCTGCGATCTATTGAAAGTCAGCCCTCAGATCGGAAGAGCACACGTC715990.13366272071131663No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACG700110.13069820444028532No Hit
GGGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAA679860.1269178861475659Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA678120.12659305879796928Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTAACCTAGACTTACAAGTCAAAGTAAGATCGGAAGAGCACACGTCTGAA661910.12356693733994552Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT659770.12316743703641862No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCAGATCGGAAGAGCACACGTC654470.1221780203968427No Hit
TTGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT641190.11969887832635807Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAACCTAGACTTACAAGTCAAAGTAAGATCGGAAGAGCACACGTCTGAA640140.11950286182229114Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAAC639510.11938525191985097Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA636020.11873373039680947Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTG607830.1134711539685744Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG600940.11218491233712569No Hit
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC585420.10928760172463162No Hit
TTCCGGCCGTGCCGGTGGTCCCGGCGGATCTTTAGATCGGAAGAGCACAC582810.10880036070023667No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT569510.10631748498205554Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC567970.10602999410942403Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA566180.10569583264058612No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA556710.10392795045628721Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTGGTGACTCTAGATAACCTCGAGATCGGAAGAGCACACGTCTGAACT553640.10335483553487249Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGAGATCGGAAGAGCACACGTC550220.10271638177876877No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)