FastQCFastQC Report
Tue 11 Jul 2023
SRR11869328.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11869328.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45708712
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG32083757.019176125549108Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT9476882.0733202895763068Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG7007311.533035977911607Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA5501751.2036545680832134Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC5481851.1993009122637277No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA5100501.1158704275018732Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG4947021.082292583523246No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG4110600.8993033975667484Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA3899810.8531874623813508Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG3708260.8112807904103708No Hit
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA3429530.7503011679699048Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT2802880.6132047649909715No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA2702220.5911827049513011Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC2576990.5637853020229492No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT2561260.5603439449354862No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG2430040.5316360697278015No Hit
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC2251010.4924684817196337Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA2005690.43879818796906817Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1996800.436853263334132No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATG1967850.4305196786118148TruSeq Adapter, Index 2 (97% over 36bp)
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT1846240.4039142472445953No Hit
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT1784840.3904813594397497No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG1570930.3436828410303926Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1557300.34070091495905636No Hit
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTC1473330.3223302376142211No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTCTGA1469360.3214616942170674Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC1425480.3118617737467641Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGAGATCGGAAGAGCACACGT1400940.30649299415831277No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1361930.2979585160920745No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG1201580.26287767636069026No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC1191640.26070303621769086No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC1190680.26049301061031865No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT1017810.22267308691612223Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA979450.21428081368820895Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC966860.21152641535819253Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG883440.1932760651842476Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC822890.1800291375525961TruSeq Adapter, Index 16 (100% over 35bp)
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC821110.17963971507226018Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCGCTGCGATCTATTGAAAGTCAGCCCTCAGATCGGAAGAGCACACGTC795020.17393183163857254No Hit
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTTTAGATCGGAAGAGCACACG738410.16154688410384435No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT725230.1586634075359638Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA701500.1534718370537328Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGA700400.15323118271195216Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG658690.14410600762497966No Hit
GGGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAA653590.142990246585815Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT653460.14296180561815Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGT644140.14092280701324508No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA634550.13882473870626674Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT616720.13492395060267723No Hit
CTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA602570.13182826066068107Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTG602550.13182388512719415Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCGGCCGTGCCGGTGGTCCCGGCGGATCTTTAGATCGGAAGAGCACAC584410.12785527625455734No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA583030.12755336444395984Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAACCTAGACTTACAAGTCAAAGTAAGATCGGAAGAGCACACGTCTGAA546410.11954176262940859Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT531980.11638481521859553No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG531800.11634543541721323No Hit
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT515920.11287126182859845No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC498450.10904923332777348Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC495070.10830976816848395No Hit
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT466120.10197618344616667Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAACCTAGACTTACAAGTCAAAGTAAGATCGGAAGAGCACACGTCTGAA463060.10130672682266786Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)