FastQCFastQC Report
Tue 11 Jul 2023
SRR11869330_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11869330_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14083647
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG6108944.337612267617898Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG3916742.781055219574873No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC3691472.6211037524584366No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2340451.6618209757742437Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1397010.9919376706899854Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1385590.9838289755487339Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTTTAGATCGGAAGAGCACACG1348330.9573727600528471No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1290560.9163535552971471Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC1219050.8655783548110798No Hit
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA1051110.7463336733730972Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCGGCCGTGCCGGTGGTCCCGGCGGATCTTTAGATCGGAAGAGCACAC978650.6948839316975213No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC941010.668157899725831No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG909320.6456566257305371No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA903870.6417868894328295Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT895550.6358793286994483No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG870520.6181069434642887Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTTAGATCGGAAGAGCACACGT813900.5779042885695729No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG804890.5715067979196014No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG662000.47004870258392584No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC635200.451019540606208No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG633450.4497769647307973No Hit
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC610680.43360927748331096Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCGGCCGTGCCGGTGGTCCCGGCGGATCTTAGATCGGAAGAGCACACG602150.4275526076448806No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGAGATCGGAAGAGCACACGT567350.4028430988081425No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC514950.3656368268815599No Hit
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA473760.33639014099117936TruSeq Adapter, Index 7 (100% over 35bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA444370.31552196671785365Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTAGATCGGAAGAGCACACGTC438790.31155992478368705No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCTCGTATG436210.30972801292165303TruSeq Adapter, Index 7 (97% over 35bp)
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA421850.2995317903097117Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG416660.2958466652849223No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG409120.2904929383702957Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT371870.26404382330798265Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA355650.2525269200513191No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC351340.24946663318102194Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCGGCCGTGCCGGTGGTCCCGGCGGATCTAGATCGGAAGAGCACACGT329720.23411549579451968No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC310010.22012054122060856Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT308480.21903417488382096No Hit
TCCCCCGCCTCCCCTCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT299490.21265088510099692Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTCTGA292460.2076592802986329Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA291960.20730425861994412Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT291530.20699893997627178No Hit
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT288280.2046912990647948No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG256680.1822539289716648No Hit
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC255420.18135927434136911Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTC238710.16949444983959056No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT232560.16512768319171875Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT230060.16335257479827492Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCCCCCGGGCCGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA201000.14271871483288384Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCGCTGCGATCTATTGAAAGTCAGCCCTCAGATCGGAAGAGCACACGTC200020.14202287234265387No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT196610.13960162449399646No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGT186040.13209646620651597No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG185180.1314858289191713No Hit
ATAACCTAGACTTACAAGTCAAAGTAAGATCGGAAGAGCACACGTCTGAA178890.12701965620126662Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACA172450.12244697697975532No Hit
TGCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGA171780.12197124793031236Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA166680.11835002680768696Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT165560.11755477824742414No Hit
TCCGGCCGTGCCGGTGGTCCCGGCGGATCAGATCGGAAGAGCACACGTCT164970.11713585266657138Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA159670.11337262287247046Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG155740.11058215247797677Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGGAGATCGGAAGAGC154570.10975140174984506No Hit
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC152180.10805439812571274No Hit
GGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGA150880.10713134176112196Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAC150760.10704613655823667TruSeq Adapter, Index 7 (97% over 35bp)
TCCGGCCGTGCCGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA149820.10637869580230179Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTAACCTAGACTTACAAGTCAAAGTAAGATCGGAAGAGCACACGTCTGAA149590.10621538583010495Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT147250.10455388437384153Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA141080.10017291685882215No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)