FastQCFastQC Report
Tue 11 Jul 2023
SRR11869332_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11869332_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13892745
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG6945524.99938637036813Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC4386853.1576553085801256No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG2662271.9163023578133769No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2236571.6098834319639495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1968321.4167970404696841No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1816321.307387416957556Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1534351.1044253673410114No Hit
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT1470561.058509315473652Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC1087740.7829554202571198Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA1067440.7683434771170132Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG993680.7152510177074437No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG983130.7076571260755165Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG921670.663418208568573No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT912850.6570695712042509No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG901340.6487846714238259No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC827610.5957138060188969No Hit
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA761830.5483653518437142Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC750410.5401452340772108No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC697600.5021325879082932No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA679730.4892697591440712Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA583830.42024092430977467Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC581140.4183046618936718No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA579820.4173545256894876Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGAGATCGGAAGAGCACACGT551760.39715693334902497No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACACG527740.37986733363349No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT497970.35843888302851595No Hit
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT454880.32742269436313703No Hit
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT431080.3102914506816328No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTCGTATG416720.2999551204603554TruSeq Adapter, Index 10 (97% over 36bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA387830.2791600939915042No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG364240.2621800083424838No Hit
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC354270.2550036008002738TruSeq Adapter, Index 5 (97% over 35bp)
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC330870.23816027718064356Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTC319910.23027126748529536No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT314260.22620439661132485Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTCTGA313530.22567894249840476Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG286090.2059276262538469No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG268710.19341749956542065Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGA258400.18599635997061775Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACAC251950.18135364897289916No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGT238810.17189547494033758No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA224800.16181107477319998No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA222260.15998278238029995Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC219770.15819047999513416Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC215980.1554624374088778Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGCGTGCATTTATCAGATCAAAACCAACCCAGATCGGAAGAGCACACGT215080.15481461726966125No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG208180.14984799620233438No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACAC207870.149624858154382No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC202350.1456515613005205No Hit
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA191690.13797849165157786No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA185650.13363089871728015Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT185080.13322061262910967Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG182140.1311044001743356Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA180320.1297943638928088No Hit
CTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC179770.129398473807732Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA177620.12785090347515915No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG173380.12479895081929453No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC169810.12222926426706889No Hit
CCCCCCCGGGCCGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA166750.12002667579373263Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGAGATCGGAAGAGC164610.11848630346270661No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACG163670.11780969131730265No Hit
TCCCCCGCCTCCCCTCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT162270.11680197110074358Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC161700.11639168501257312No Hit
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC154950.111533033968449No Hit
CTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCAC153170.11025178969310961No Hit
TTGCATTTATCAGATCAAAACCAACCCAGATCGGAAGAGCACACGTCTGA151770.10924406947655053Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGATCGGAAGAGCACACGTCTG151430.10899933742395762Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCAG148150.10663839291659064Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTGA144950.10433503242159847Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT144750.10419107239066146Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGAGATCGGAAGAGCACACGTC140190.10090878368529761No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)