FastQCFastQC Report
Tue 11 Jul 2023
SRR11869334_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11869334_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13397224
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG10858478.105014889651766Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC4708883.514817696561616No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG2098071.566048309709534No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1905201.4220856499824142No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1895931.4151663060944566Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1734231.2944696602818613Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1544701.1530000543396155Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1526481.139400222016143No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG1525421.1386090133299256No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1298600.9693052829451833Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA1213420.9057249471980164Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC1130780.844040526604616No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1014210.7570299638193704Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA916200.6838730172758177Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA856710.6394682958200893Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG844720.6305186805863662Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC787910.5881143735448479Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG711520.5310950985069742Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGAGATCGGAAGAGCACACGT690430.5153530313444039No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG632210.4718962674655585No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT608980.45455685446477573No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC588980.4396283886870892No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG565320.42196801367208614No Hit
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT547070.40834578864994714No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT527080.39342478710514955Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA444760.331979221964192No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTCTGA417700.3117810077669822Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC375580.28034165883917445No Hit
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTC374310.27939370126229135No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG373360.2786845991378512No Hit
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT368380.2749674111592073No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT345190.25765785508997985No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC335000.2500518017762486No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG333010.24856641943136878No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATG308240.23007751456570405TruSeq Adapter, Index 9 (97% over 36bp)
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT296760.22150857520931203Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG292770.21853034628666357Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGCATTTATCAGATCAAAACCAACCCGAGATCGGAAGAGCACACGTCTG286380.21376070147069273Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT284260.21217828409825795Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG276540.20641589630807097No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT257930.19252495890193372No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGAGATCGGAAGAGCACACGTC234550.17507358240781823No Hit
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC227910.1701173317696263Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA217230.16214553104434173No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG216220.16139164352256855No Hit
AATGAGTCCACTTTAAATCCTTTAACGAGATCGGAAGAGCACACGTCTGA212970.15896576783369448Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG192550.1437238042746766Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC191960.14328341453423488No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC186150.13894669522581693No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC182370.13612521519383416No Hit
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA180500.1347294036436205No Hit
TTAACCTAGACTTACAAGTCAAAGTAAGATCGGAAGAGCACACGTCTGAA163440.12199542233525393Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG159880.11933815542682574No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT158240.11811402123305544No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT157140.1172929556152827Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC154230.11512086384462931Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT153650.11468793833707638Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCT144880.10814180609356087Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCGTGCATTTATCAGATCAAAACCAACCCAGATCGGAAGAGCACACGT144280.10769395212023028No Hit
TTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGA141040.10527554066424506Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCCCAGATCGGAAGAGCACACGTCTG139740.10430519038869546Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)