FastQCFastQC Report
Fri 7 Jul 2023
SRR11869336_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11869336_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20651731
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG17506278.476902008843714Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC4556662.206430056637867No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATG4086841.9789333881987905TruSeq Adapter, Index 3 (97% over 36bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT3917871.897114580855232Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA3225411.5618109687754504Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG2600441.2591874259838074No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2497611.2093949896984422Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG2464111.1931735891775852Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA2186751.0588700772831101Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT2156001.0439802842676964No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC2116391.0248002939801997No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA2079801.007082650844135Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1871190.9060693265857471No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1834890.8884921075138932No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1742980.8439873635774163Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG1319610.638982756457558Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA1143090.553508081235418Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC1076830.5214236036678959Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT987870.4783473114190767No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT937900.4541507924928908Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG891800.43182820849254716No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG875780.42407098949719996No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA872790.42262316897309965Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT841300.40737505248349404No Hit
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT835410.4045229913172896No Hit
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTC825720.39983089068901784No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC739700.35817820791874544No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG713440.34546256679403775No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC697700.3378409296537903No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTCTGA673500.32612278360588753Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG576020.27892092919474887No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGAGATCGGAAGAGCACACGT534850.25898555428598213No Hit
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC518990.2513058106364062Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC467710.22647496231671815No Hit
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG460740.22309994256655774Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC437770.21197738823927154TruSeq Adapter, Index 5 (97% over 35bp)
TTGCATTTATCAGATCAAAACCAACCCGAGATCGGAAGAGCACACGTCTG434900.21058767422449964Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA405200.19620631316570994No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT384240.1860570428696752No Hit
TTGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT376670.18239149057287254Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCCCAGATCGGAAGAGCACACGTCTG375090.18162642153338138Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGAGTCCACTTTAAATCCTTTAACGAGATCGGAAGAGCACACGTCTGA364110.17630967592982882Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG333890.16167651999728255Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG319070.15450036609521983No Hit
TCCCCCGCCTCCCCTCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG295800.14323254549461253Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG293430.14208494193537577No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG279610.1353930089443834No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC271910.13166450792914164Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATGTCGTATG270430.13094786098075747TruSeq Adapter, Index 3 (97% over 36bp)
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA267500.12952909371132135Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT266840.1292095079100149No Hit
TGCATTTATCAGATCAAAACCAACCCGAGATCGGAAGAGCACACGTCTGA266590.1290884526822473Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC263070.12738399507527964No Hit
TCTCCCCTACTTCCTGAGAAGACTCCAACAGATCGGAAGAGCACACGTCT263000.1273500996115047Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC261430.12658987278112427Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTG252310.12217377807216256Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC250450.1212731271775717No Hit
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT242380.11736546442523389Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGAGATCGGAAGAGCACACGTC221270.10714356099253859No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCT219170.10612669707929083Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGGCCGTGCCGGTGGTCCCGGCGGATCAGATCGGAAGAGCACACGTCT215790.10449003039987301Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGGCCGTGCCGGTGGTCCCGGCGGATCTTTAGATCGGAAGAGCACACG210720.10203503038074628No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)