FastQCFastQC Report
Tue 11 Jul 2023
SRR11869338.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11869338.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45025479
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG40417928.976677405253145Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA8634371.917663108037118Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA8336381.851480580584162Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG7952121.7661377905607623No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT6172771.3709504345306354Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA5732941.2732657436026389Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG5341241.1862705558335094No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT4909461.0903737414986745No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG4692441.042174365318801Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA4609551.023764788821014Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT4524101.0047866453569543No Hit
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA3940210.8751067367878529Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG3767900.8368372938353414Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG2987840.6635887205108911No Hit
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC2841900.6311759615039298Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC2726500.6055460287274235No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC2297970.5103710279239895Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG2284140.5072994337272904Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTCTGA2128220.47267015193775064Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1986290.44114799978030217No Hit
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA1914180.42513262324205364Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT1618020.359356532331394No Hit
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1585700.35217837438220256Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG1551890.34466929269092283No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1501190.33340900160107123No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1499830.33310695040023897No Hit
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT1408740.31287618283861013No Hit
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTC1388140.3083009955318854No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG1386040.3078345929423649No Hit
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1143750.2540228389352615Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT1097000.24363982890665084Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG925670.20558804049591564No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC834000.1852284569809907Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT831060.18457549335566203Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCT826520.18356717537641298Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGAGTCCACTTTAAATCCTTTAACGAGATCGGAAGAGCACACGTCTGA815820.18119074313457054Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT749520.1664657470939954No Hit
TTGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT684060.1519273120892284Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC664380.1475564535360079No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG663390.1473365780295197No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC653610.14516447454118145No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATG644670.1431789320886514TruSeq Adapter, Index 7 (97% over 35bp)
TTGCATTTATCAGATCAAAACCAACCCGAGATCGGAAGAGCACACGTCTG627660.13940107111353553Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA626150.13906570544202318Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGAGATCGGAAGAGCACACGT597490.13270042057742462No Hit
TTAACCTAGACTTACAAGTCAAAGTAAGATCGGAAGAGCACACGTCTGAA583800.12965991988669348Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA568090.1261707843241379Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG565350.12556223999304927No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT560100.12439623351924807Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAAC553240.12287265172681451Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG525270.1166606134273441No Hit
TCTCCCCTACTTCCTGAGAAGACTCCAACAGATCGGAAGAGCACACGTCT524500.11648959914451994Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA511090.11351128546572486Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGA502380.11157682520157088Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC473550.10517378393686827No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA471360.10468739266493979Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGGAAGGTGGCTCGGGGGGGGCGAGATCGGAAGAGCACACGTCTGAA468570.10406774351029115Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAA456610.10141146971473641Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC451400.10025434709978322No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)