FastQCFastQC Report
Tue 11 Jul 2023
SRR11869340.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11869340.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33359579
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG6377991.9118916338842287Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA4874431.4611785118751048Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA3174660.9516487003627953Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA2745910.823124896150518Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTGA2497060.7485286310118002Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATG2454270.7357017305284338TruSeq Adapter, Index 7 (97% over 36bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT2139490.6413420265285722No Hit
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA1793570.53764767235222Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGCTAACTAGTTACGCGACCCCCGAGATCGGAAGAGCACACGTCTGAAC1786020.5353844543421846Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1753060.5255042337314868Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1636080.49043784395480533Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCT1631300.4890049721550742Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1526750.4576646485856431No Hit
TAGTGACGAAAAATAACAATACAGGACAGATCGGAAGAGCACACGTCTGA1521790.45617781927044104Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACCTATTCTCAAACTTTAAATGGGTAGATCGGAAGAGCACACGTCTGA1489000.44634855853546596Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCTGAA1479540.4435127913334878Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTG1466370.43956489978485636Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1429090.4283896988028536Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTATCAGATCAAAACCAACCCGAGATCGGAAGAGCACACGTCTGAACT1351280.4050650639206208Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT1343120.40261898988593353No Hit
TCGGTCACGCGTCTCCCGACGAAGCCGAGCGCAGATCGGAAGAGCACACG1316600.39466924927319974No Hit
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC1295400.38831425300661015Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATGCTAACTAGTTACGCGACCCCCGAGATCGGAAGAGCACACGTCTGAA1294590.38807144418699047Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1271290.38108694357323875No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1259740.3776246696638468Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1172360.3514312935424035No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT1154460.3460655183927831No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1017330.30495888452309305Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCACACGTCTGAA1015050.3042754226604598Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA975960.2925576488839982Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC965740.2894940610611423Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT963990.2889694741051738Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTCTGAAC963050.28868769596882504Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATTATTCCCCATGAACGAGGAATTCCCAGTAGATCGGAAGAGCACACGT918660.275381173125716No Hit
TCAACCTATTCTCAAACTTTAAATGGGTAGATCGGAAGAGCACACGTCTG865340.2593977579872936Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG860840.25804882010051744No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGATCGGAAGAGCACACGTCTGAA843480.25284491749731014Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA841760.25232932346058684TruSeq Adapter, Index 7 (100% over 35bp)
ACATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTGAA793170.23776379192315347Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCTCACCCCGAGTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGT780660.23401374459791593Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TACCTGGTTGATCCTGCCAGTAGCATAGATCGGAAGAGCACACGTCTGAA761230.22818933056679164Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGCCGCCGGTGAAATACCACTACTCTCAGATCGGAAGAGCACACGTCT731760.21935528622828243Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG723460.21686724523711765Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA715400.21445114759991424Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGACGAAAAATAACAATACAGGACAGATCGGAAGAGCACACGTCTGAA709140.21257462511742128Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACACGTCTGAA690940.20711892077534913Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGTCACGCGTCTCCCGACGAAGCCGAGCAGATCGGAAGAGCACACGTC671000.20114162711705685No Hit
AGTTATGGTTCCTTTGGTCGCTCGAGATCGGAAGAGCACACGTCTGAACT662580.19861761444891135Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCAC658670.19744553730729036No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACAC629600.18873139855871682No Hit
ATGCTAACTAGTTACGCGACCCCCAGATCGGAAGAGCACACGTCTGAACT628590.1884286369441293Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC625010.1873554819142052No Hit
TAGACGACCTGCTTCTGGGTCGGGGTTTCGTAGATCGGAAGAGCACACGT594080.1780837821724309No Hit
CCGCCTGGATACCGCAGCTAGGAATAGATCGGAAGAGCACACGTCTGAAC573290.17185168913552534Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGCCATGCATGTCTAAGTACGCACGGAGATCGGAAGAGCACACGTCTGA568430.17039483621780718Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG559660.167765906158468No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGAGATCGGAAGAGCACACGT557070.16698951746363466No Hit
AGAGCGAAAGCATTTGCCAAGAATGTTTTCAGATCGGAAGAGCACACGTC553190.16582643324125884No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTAGATCGGAAGAGCACACGTCTGAA550810.16511299498114168Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG549940.16485220032303166Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGAA546310.16376405709436562Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGGAATGAGTCCACTTTAAATCCTTTAGATCGGAAGAGCACACGTCTG538330.16137194057514934Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCTGA523320.156872483312814Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA515960.15466622045799797Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCGGTCACGCGTCTCCCGACGAAGCCGAGCGCAGATCGGAAGAGCACAC512270.1535600913908416No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGATCGGAAGAGCACACGTCTG505570.15155167275941941Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAGCCATGCATGTCTAAGTACGCACGGAGATCGGAAGAGCACACGTCTG502660.15067935959263756Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT488990.14658158605658664No Hit
TATGCTAACTAGTTACGCGACCCCCAGATCGGAAGAGCACACGTCTGAAC480620.1440725615871831Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTGAACT476760.14291547264430404Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCTCACCCCGAGTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA456500.13684225451406326Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCTGGATACCGCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT455580.13656647165721125Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAAATCTCGCGCCGGGCCGTACCCAGATCGGAAGAGCACACGTCTGAACT454650.13628769116061087Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG443000.132795440853735TruSeq Adapter, Index 7 (100% over 36bp)
CCGGGGAGGTGGAGCACGAGCGTAGATCGGAAGAGCACACGTCTGAACTC438440.13142851712846856Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATATGCTTGTCTCAAAGATTAAGCCAGATCGGAAGAGCACACGTCTGAAC437390.13111376495488747Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTGAA431880.12946206545352384Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGGTTGGCCCCGGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC430610.12908136520547817Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACAGCCAAGGGAACGGGCTTGGCGGAATCAGATCGGAAGAGCACACGTCT426530.1278583281881345Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGCCGCCGGTGAAATACCACTACTCTCAGATCGGAAGAGCACACGTC421070.12622161688551284No Hit
AAAAGCGAATGATTAGAGGTCTTGGGGAGATCGGAAGAGCACACGTCTGA419100.12563108185507976Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCAGATCGGAAGAGCACAC409480.12274735241712732No Hit
AGGAGCCTCGGTTGGCCCCGGATAGATCGGAAGAGCACACGTCTGAACTC405260.12148234844330619Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTGA402510.12065799751249859Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTCAACCTATTCTCAAACTTTAGATCGGAAGAGCACACGTCTGAACTC394860.11836480310497924Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTAACCTATTCTCAAACTTTAAATGGGTAGATCGGAAGAGCACACGTCTG391500.11735759614951975Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGAGATCGGAAGAGCACACGTC389240.11668012956638331No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAGATCGGAAGAGCACACGTCT387820.11625446472211176Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAATCTCGCGCCGGGCCGTACCCAGATCGGAAGAGCACACGTCTGAACTC386260.11578683292136271Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTCTGA386140.11575086124438201Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTACGAGAGGAACCGCAGGTTCAGACAGATCGGAAGAGCACACGTCTGA382180.11456379590401905Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTTAAATCCTTTAACGAGGATCCAGATCGGAAGAGCACACGTCTGAAC376130.11275022385624231Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG368780.11054695864117471No Hit
AAATTACCCACTCCCGACCCGGGGAGGTAGATCGGAAGAGCACACGTCTG368250.11038808373450995Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC368150.11035810733702603No Hit
ATCTCAACCTATTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC363840.1090661246054694Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTTGCCAAGAATGTTTTCATTAATCAGATCGGAAGAGCACACGTCTGAA363350.10891924025779821Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAATCCCGCCCAGGCGGAACGATACAGATCGGAAGAGCACACGTCTGAA360930.10819381143868752Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGAGGTGTCAGAAAAGTTACCACAGATCGGAAGAGCACACGTCTGAACT360350.10801994833328082Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG359990.10791203330233873No Hit
TCCGGCCGTGCCGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA349490.10476451156652786Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTTGGTGACTCTAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA347570.10418896473483673Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TACCTGGTTGATCCTGCCAGTAGCAGATCGGAAGAGCACACGTCTGAACT346000.1037183352943393Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)