FastQCFastQC Report
Fri 14 Jul 2023
SRR11869342.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11869342.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28449692
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA8245492.898270392523054Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG7243222.545974838673122Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA6752812.3735968740891815Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG4978191.749822107037222Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA4681481.645529238066971Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT4120671.448405838629114Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC3997901.405252471626055Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA2251860.7915235075304154Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG2163940.7606198337753534No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2157090.7582120748442549Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1853380.6514587222947792No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1849790.650196845716291Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA1773970.6235462935767458Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC1733080.609173554497532No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT1630550.5731345000149738No Hit
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC1428800.5022198482851765Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1298490.45641618896963804No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAAC1190090.4183138432570729Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1183890.4161345578011882No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1068590.37560687827481576Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT1036200.36422186925608896Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC958310.3368437169724017No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA903750.3176660049606161No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC883040.31038648854265277Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA871130.30620015148142904Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA742040.260825319303984Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG610990.21476155172435613No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC602990.21194957049095647No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA593480.20860682779975265Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC584810.20555934313805577No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT522360.1836083146348298No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC503700.17704936840792512No Hit
TCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAAC502180.1765150919735792Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG501970.17644127746620245Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT490690.17247638392710896Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC468470.16466610605134144No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACAC465310.16355537346414858No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC465240.16353076862835633No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA456220.16036025978769822Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC405310.14246551421365122Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA405140.1424057596124415Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA383990.13497158422664118Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG371870.13071143265804072No Hit
CCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACA364870.12825094907881604No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT362150.12729487545946017Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGA358630.1260576037167643Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA344190.12098197759047796Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC337280.11855312880012903No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACAC331990.1166937062095435No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC330150.11604695052586159No Hit
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC313970.11035971848131079Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT308260.1083526668759718Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG301960.10613823165466958No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT297930.10472169610834452No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG292530.10282360877579975No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG284500.10000108261277486No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)