FastQCFastQC Report
Tue 11 Jul 2023
SRR11869348.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11869348.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34865200
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG11892743.411063180477955Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC9142032.622107430905315Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC7132592.045761963218338Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA7122262.0427991234812937Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA6776611.9436601539644116Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC4554891.3064287599095947No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG4260181.2219003476245656TruSeq Adapter, Index 9 (100% over 49bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT4241111.2164307102784437No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG3268200.9373816871837821No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT3174640.9105469063708225Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA3133160.8986496563908998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG2638720.7568348955405391No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT2493360.7151428932000964No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2459180.7053394215435448Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA2436090.6987167720248271Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG2359440.6767320996294299Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG1768780.5073196195633468No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC1637770.4697434691325448No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1564380.4486938265089545Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG1418270.4067867099572066Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA1366400.39190941110333516Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT1113250.3193011943141012Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA1004450.28809529272741874TruSeq Adapter, Index 9 (100% over 35bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG963970.2764848616959031No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT938330.26913082385874737No Hit
TTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTC841350.2413151222422358Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG834620.23938483071945663No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG812450.23302605463327328No Hit
CTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC776550.22272925438546173Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC770510.2209968679370834No Hit
CACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGT748850.21478436951458763Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC745200.21373748035290202Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA705060.20222456776384473Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG673180.19308077968862936No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC623990.17897215561648866No Hit
CTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA618240.17732294666314835Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG599990.17208850085472047No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT589240.16900519715934514No Hit
TCCGGCCGTGCCGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA580030.1663635946445166Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGA564210.16182611888071774Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAAC559990.16061574291844016Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT552910.15858506476371856No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC540490.15502277342450352Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGAGATCGGAAGAGCACACGT532520.15273682640569966No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA515840.1479526863462708Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG500580.14357582919357986No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGAGATCGGAAGAGCACACGTCTGAA494540.14184344274520153Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA479870.13763580877207074Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC459200.13170726110849787Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT453050.12994332457579477Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC421940.12102038709085278No Hit
GTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA415720.11923637323176119Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACAC391790.1123727957963815No Hit
TTCCGGCCGTGCCGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC381610.10945297890159816Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA352270.10103771095533655Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG349240.10016864954166332Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)