FastQCFastQC Report
Tue 11 Jul 2023
SRR11869352_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11869352_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20597844
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA12984696.303907340981901Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA9083054.409709093825548Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG6404663.109383681127015No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG6179873.000250900045655Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA5122992.487148654975734Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG4382812.127800365902373Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC3051521.4814754398567151No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC2190981.063693850676799No Hit
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC1653210.8026131278594011No Hit
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA1560360.7575355945020265Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1554390.754637232906512Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACA1539290.7473063685694483No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG1433420.695907785300248Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA1381850.67087118438221No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC1361840.6611565754163398No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1183620.5746329567308113No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT1123210.5453046445055123No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAAC1063150.5161462529767679Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG1019250.4948333427517948No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1007830.48928907316707515Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT975960.473816580026531No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG843810.40965937988461315No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG815190.3957647217835032No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC803940.39030298510853856No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA743630.3610232216536838Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCAC730310.3545565254305257No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC726180.3525514612111831No Hit
ATTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT718260.34870639859200797Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT691050.3354962781541602No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT682210.3312045668468991Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC668490.3245436755419645No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACAC612320.29727383118349665No Hit
ATTAAGCCATGCATGTCTAAGTACGCACGGAGATCGGAAGAGCACACGTC612280.297254411675319No Hit
CTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA608270.2953076059805094Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC584240.283641336442785Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG532610.25857560626248066Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG525400.2550752399134589No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGAGATCGGAAGAGC503560.24447218844846091No Hit
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC498630.24207873406556532Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT474230.23023283407719758Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA447870.21743537818812494No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT431580.20952678348277617No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG415780.2018560777526036No Hit
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTC397430.19294737837610576No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC394950.19174336886909135No Hit
CGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGTC384510.18667487723472417No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCT375790.18244142445199604Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC366500.17793124367773638No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG364830.1771204792113194No Hit
TCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAAC344930.16745927389293752Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGAGATCGGAAGAGCACAC339390.16476967201033274No Hit
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC338400.16428903918293583Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAA338300.16424049041249172Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA317790.15428313759440065Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACAC313430.1521664112030366No Hit
CCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAAC311880.15141390526115256Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA305300.1482193961659288Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT298820.1450734358411492Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC293310.14239839858967762No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC291080.14131576100877355No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG284480.13811154215946095No Hit
CCGGGCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTC267620.12992621946258065No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT267110.1296786207333156No Hit
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA263820.1280813661857037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGGGCGTCGCGGCCGCTCCCGGGGAGAGATCGGAAGAGCACACGTCT257450.12498880950841264Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGA243860.11839103160505536Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA237410.11525963591140899No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC230290.11180296345578694No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA226200.109817318744622No Hit
CCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCA222470.10800644960705595No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT219700.10666164866575358No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA214920.10434101743852416Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACT210130.10201553133425033Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACA209380.10165141555591933No Hit
TCCTCCCGCCCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG207060.10052508408161552Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)