FastQCFastQC Report
Tue 11 Jul 2023
SRR11869356.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11869356.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48036542
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG17940833.734829621999019Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG7496971.5606806168520624No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT6396171.3315217402618198No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT6182521.2870451832273855Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA6157461.2818283214474515Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC5877101.223464420065874No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA5164961.0752147812804675Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA4911131.0223737587106083Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG3427340.7134859957238388TruSeq Adapter, Index 22 (97% over 37bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC3261350.6789310521144507No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA2907870.6053454055872715Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC2793420.5815197938269578Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG2623980.5462466469797097No Hit
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT2540610.5288911096056831Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA2459700.5120476823664785Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG2340580.48724989404940927Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC2297850.47835458264252245Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG2107220.4386702106908528Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1970300.4101669100161289No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG1881120.39160187675457575No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG1785630.371723260179719No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1737380.36167882359225606Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG1678350.3493902621050449No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGAGATCGGAAGAGCACACGT1543460.32130955637897496No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT1078980.22461650132934216Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTC1000100.20819566903879133No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC950830.19793889410274368No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG935360.19471842914920895No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG894500.1862124047147274No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT893500.18600422986317375No Hit
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG873730.18188861304795836TruSeq Adapter, Index 11 (100% over 35bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGAGATCGGAAGAGCACACGTC862990.17965281514227233No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA861300.17930099964314666Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT835480.1739259249760318No Hit
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT765550.15936825760688603No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG755960.15737186078048665No Hit
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA702210.14618246250947872No Hit
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT701530.14604090361042224No Hit
ACTTTTTCTGGAGCAAATCCATGTCTTGAGATCGGAAGAGCACACGTCTG692400.14414026721573756Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC689980.14363648407497775No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC662320.13787836768100417No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG655440.13644612470231518No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT613970.12781311360838588No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAAC601430.1252026009699033Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA583420.1214533718934223Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGAAGGTGGCTCGGGGGGGGCGAGATCGGAAGAGCACACGTCTGAA575760.11985875253052146Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC562020.11699843007017449No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC547690.11401528444741088No Hit
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC532390.11083020921864026Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC504520.10502837610584041No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC504360.10499506812959183No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACAC495810.10321517314880826No Hit
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG493820.10280090519421653Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGA489250.10184954612261639Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA483590.10067127646282283Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC480440.10001552568042887Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)