FastQCFastQC Report
Tue 11 Jul 2023
SRR11869360.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11869360.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43850949
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA13531053.085691486403179Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA13449033.066987216171764Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG13043442.9744943490276574Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA7587731.7303456762133014Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG7307481.6664359989107647Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT5639581.2860793503009478No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG5638591.2858535855176134No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT5295961.207718446412642No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC5081531.1588187065233184Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT4660361.0627728946071384Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT4172860.9516008422075426Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA3746250.8543144642092011Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC3009160.6862246014333692No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2969280.677130157433993No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG2725940.6216376297808287Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG2318030.5286156976899177No Hit
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC2167460.4942789265518518Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC1862260.4246795206188126No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA1785800.4072431818978422No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1770680.40379513793418703Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1670430.3809336030561163No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1633710.37255978200152523Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATG1592000.36304801522083363TruSeq Adapter, Index 1 (97% over 37bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC1574660.3590937108339434Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAAC1459370.33280237561107284Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG1421650.3242005093207903Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA1180870.2692917774709961Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC1051650.23982377211494327No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1041410.23748858890146254No Hit
TCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAAC974480.22222552127663192Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG968980.22097127248032877No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC898960.20500354507721144No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACAC799620.18234953136361998No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT789110.17995277593650252Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG777830.17738042567790266No Hit
TGCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGA729550.16637040169871808Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTC711690.16229751378926827No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC694700.15842302523487006No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC685140.15624291278165953No Hit
CTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA682960.1557457741678521Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA680690.15522811148283244Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC673920.15368424523720114Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG672820.15343339547794052No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG672720.15341059095437135No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT636160.14507325713749092Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT636130.14506641578042018No Hit
ACTTTTTCTGGAGCAAATCCATGTCTTGAGATCGGAAGAGCACACGTCTG628990.14343817279758303Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACAC620660.141538555984273No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC599480.1367085578923275No Hit
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT597680.1362980764680828TruSeq Adapter, Index 1 (100% over 35bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC596440.1360153003758254No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA576380.13144071294785434Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT576170.13139282344835912Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGGCCGTGCCGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA573310.13074061407428147Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACA571230.13026627998404322No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG560860.12790145088992258No Hit
CCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAAC541490.12348421467457864Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACAC540130.12317407315403824No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC535690.12216155230756807Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAA524310.11956639752539905Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACT523340.11934519364677831Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATG520650.11873175196276825TruSeq Adapter, Index 15 (100% over 36bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG519940.11856983984542728No Hit
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC486870.1110283839011101Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC482710.11007971572063356No Hit
ATTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT460310.10497150244114443Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG458130.104474363827337No Hit
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC452830.10326572407817218Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACACG445230.10153258028691695No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)