FastQCFastQC Report
Tue 11 Jul 2023
SRR11869366.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11869366.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45185405
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT15325593.391712434579263Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG15072403.3356788547098337Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG14104033.121368503834369No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG13739123.0406101262122136Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA11939282.6422868180555206Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT8351901.8483623196472403No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT6471171.432137213332491No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA5584021.2358016930466817Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA5542161.2265376397533672Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG3834840.8486899696926474No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG3769310.8341874992599934No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC3136670.694177688570015No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA3088110.6834308556048131Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG2935670.6496942984133925No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC2699160.5973521759957667Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG2559550.5664550312208113Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC2487840.55058486252364No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC2453440.5429717848052928No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC2312040.5116784944165047Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC2051950.45411787279543026Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1761460.3898294150511653Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1751200.38755877035958847Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC1601700.35447286574060805No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA1587490.35132804497381404No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1431480.3168014096587161No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA1409080.3118440567258388Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1403260.31055603020488587Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG1328200.293944471671771Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAAC1190660.26350543942230903Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC1072940.23745277927684835No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT1071190.23706548607896732No Hit
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1059640.23450935097295245Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC961990.21289839053119033No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT947750.20974693045243262Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACA943450.2087952957376392No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAAC933700.2066375193494448Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC919610.20351925583050545No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA891040.19719641773709895Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG838110.18548245832918836No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA831830.1840926290247924Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT780810.17280137247856914Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTGA777220.17200686814691601Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACAC761830.16860090110955075No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACACG754680.16701853175820822No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC747620.1654560803427567No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACAC739080.16356608953709723No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG727820.16107413444673122No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC717080.15869726076373555Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGGCCGTGCCGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA714430.15811078820694427Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG710580.15725874317160596No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC653440.14461306698479298No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG641890.1420569318787781No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG630900.13962473059608516No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC621980.1376506418388858No Hit
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA612610.1355769634022313Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA611320.13529147298779332TruSeq Adapter, Index 7 (100% over 35bp)
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC600080.13280394410540305No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG599480.13267115786612957No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT595510.13179255558293657Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACAC586380.12977199164199146No Hit
CCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACT577890.12789306635627146Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGGCCGTGCCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC557140.12330087558139624Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCT546260.12089301844257012Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGA530580.11742287138955598Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCAGATCGGAAGAGCACACGTCT523270.11580509237440718Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACA514900.11395272433654186No Hit
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTC507220.11225306047384107No Hit
CCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAACTC504440.11163781756520717Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGA502110.11112216433602842Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGCACACGTCTGA492240.10893783069997934Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCGCTGCGATCTATTGAAAGTCAGCCCTCAGATCGGAAGAGCACACGTC488830.10818316224010828No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC487680.10792865528150074No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT454690.10062762522544615Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAACCTAGACTTACAAGTCAAAGTAAGATCGGAAGAGCACACGTCTGAA454050.10048598657022108Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)