FastQCFastQC Report
Tue 11 Jul 2023
SRR11869370_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11869370_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19153011
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG11800306.161067834190666Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA6274733.2761062999441704Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA4955422.5872798799102656Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT4687712.4475055123186635No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA3776121.9715542376078623Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT3222401.6824508689521456Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG2914921.521912142169187Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT2570661.3421701684398344Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG2391851.2488114793021317No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG2224811.1615980380317226No Hit
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC2073181.0824303291007351Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1964741.0258125993871148No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1623440.847616074569163Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC1538340.8031844183663863No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT1519140.7931598848870289No Hit
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA1433040.7482061175655358Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC1427590.7453606119685307No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG1389820.7256404750146074No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1388060.7247215594456663Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1296680.6770110454173498No Hit
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA1137770.594042367542106TruSeq Adapter, Index 10 (100% over 35bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG1128400.5891501863597322Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC1061820.5543880280755856Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG829790.43324258519978925No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC772500.40333083920851925No Hit
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC733520.3829789478009489Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA699010.36496089309404145Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGGCCGTGCCGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA689060.359765887462812Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAC657410.34324107055543385TruSeq Adapter, Index 7 (97% over 35bp)
CTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAAC607760.3173182535111581Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG540790.2823524718907121No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG537170.2804624296409583No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC529080.27623855069054154No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA515920.26936756836823206Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAG498360.26019929712356976Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA460650.2405104868367694Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC428790.22387602659446076No Hit
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTC427460.2231816188065678No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCAGATCGGAAGAGCACACGTC415740.21706247649521007No Hit
CACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGT410890.2145302375694349Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGA410260.21420130756464348Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACAC407930.21298478865803397No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT389840.20353979852045195No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT384990.20100755959467675Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC380490.1986580595604524No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA356110.18592899048614342Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA346950.1811464526387No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAGCATCTCGTATG342970.17906845038620822TruSeq Adapter, Index 7 (97% over 35bp)
TTCCGGCCGTGCCGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC341950.17853589704511735Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT340360.17770574036635808No Hit
TCCGGCCGTGCCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC340200.17762220258736341Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCCCGCCTCCCCTCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG337270.176092417009524Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC326760.17060502915181325Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG312680.16325370460028452Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA310610.1621729345845413Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACAC310370.16204762791604935No Hit
TTAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT303160.15828320675010316No Hit
CTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC302130.15774543229782514Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAAC284390.1484831810517939Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGTC279420.14588828879177274No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC279350.14585174101346257No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT271910.1419672342902116Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT270890.14143468094912076Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG266670.13923137202813698No Hit
CCGGGCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTC265940.13885023091147392No Hit
TTCCGGCCGTGCCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA256170.13374920528161344Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACT255260.13327408416358139Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACA248190.12958275855425552No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC244960.1278963396408011Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCCCCCGCCGGGTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC242480.12660150406638412Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGAGATCGGAAGAGCACACGT236310.12338007846390314No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGAGATCGGAAGAGCACACGT234810.12259691178582834No Hit
GTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA233800.12206957955592465Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACA229910.12003856730411736No Hit
CGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTG229900.1200333461929302Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG228510.11930761173791421No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTGA216690.11313625831468482Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGAGTCCACTTTAAATCCTTTAACGAGATCGGAAGAGCACACGTCTGA211110.11022287827224658Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCCCCCGGGCCGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC207350.10825974046587243Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTC200010.10442744485449312Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA195710.10218236704401204Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC195600.10212493482095321No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC194100.10134176814287843No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCT193210.1008770892472207Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)