FastQCFastQC Report
Tue 27 Jun 2023
SRR1190356.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1190356.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219439441
Total Bases10.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC117037415.333471934974534RNA PCR Primer, Index 1 (100% over 33bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGA66756573.042140906656794RNA PCR Primer, Index 1 (100% over 22bp)
GAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACTC29294421.3349660328381898RNA PCR Primer, Index 1 (100% over 26bp)
ATACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTC27861591.2696710250916106RNA PCR Primer, Index 1 (100% over 26bp)
TTCCAGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT26453551.2055057139887628RNA PCR Primer, Index 10 (100% over 39bp)
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT21082690.9607520828491355RNA PCR Primer, Index 1 (100% over 25bp)
ATGCCTGTTTGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG20578930.9377954075265805RNA PCR Primer, Index 10 (100% over 36bp)
AGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGAACTC19843430.9042781876207933RNA PCR Primer, Index 1 (100% over 26bp)
AGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT18063780.823178363820203RNA PCR Primer, Index 1 (100% over 30bp)
AAGGGGAATCTGACTGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC17270770.7870403752988052RNA PCR Primer, Index 1 (100% over 31bp)
AGTTTTGGTGGCAGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC15037270.6852583077806874RNA PCR Primer, Index 1 (100% over 33bp)
AGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG14801180.6744995308295558RNA PCR Primer, Index 1 (100% over 29bp)
ACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA14660500.6680886504810227RNA PCR Primer, Index 1 (100% over 28bp)
AACTTTTCGAATCGCATGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG13308610.6064821318971552RNA PCR Primer, Index 1 (100% over 29bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGAA12841300.585186507105621RNA PCR Primer, Index 1 (100% over 23bp)
TACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCC12760150.5814884481044591RNA PCR Primer, Index 1 (100% over 27bp)
AAGGATTGGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC9710780.4425266467936363RNA PCR Primer, Index 10 (100% over 37bp)
AACTATGCCGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC9271670.4225161145940032RNA PCR Primer, Index 10 (100% over 37bp)
CCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGAAC8790520.4005897918779332RNA PCR Primer, Index 1 (100% over 24bp)
AATGACCCACTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA7676880.3498404828692578RNA PCR Primer, Index 10 (100% over 35bp)
GTGAATCATCGAATCTTTGAACGCTGGAATTCTCGGGTGCCAAGGAACTC7039470.320793288932959RNA PCR Primer, Index 1 (100% over 26bp)
AATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA7000030.31899598213066904RNA PCR Primer, Index 10 (100% over 35bp)
ACTATGCCGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT6877270.3134017279965638RNA PCR Primer, Index 10 (100% over 38bp)
AAGCGGAGGAAAAGAAACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC6551900.2985744025842647RNA PCR Primer, Index 1 (100% over 31bp)
AAGGATTGGCTCTAAGGGTCGGTGGAATTCTCGGGTGCCAAGGAACTCCA6453730.2941007309620334RNA PCR Primer, Index 1 (100% over 28bp)
TCCAGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA6341390.28898132309770147RNA PCR Primer, Index 10 (100% over 40bp)
TATACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACT6051940.27579089576700117RNA PCR Primer, Index 1 (100% over 25bp)
GAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA6039260.2752130598072386RNA PCR Primer, Index 1 (100% over 32bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCAA5928850.2701816033153311No Hit
AAGCTGGTAAGAGCCTGACCGAGTTGGAATTCTCGGGTGCCAAGGAACTC5772160.2630411367116088RNA PCR Primer, Index 1 (100% over 26bp)
GGGCCAGCATCAGTTTTGGTGGCAGGATTGGAATTCTCGGGTGCCAAGGA5755080.26226278985098217RNA PCR Primer, Index 1 (100% over 22bp)
ACGCGAAACTCAGGTGCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT5642010.25711011540537054RNA PCR Primer, Index 1 (100% over 30bp)
AATGACCCACTCGGCACCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT5456510.24865675810758192RNA PCR Primer, Index 1 (100% over 30bp)
AACTTTTCGAATCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA5111030.2329130067370159RNA PCR Primer, Index 10 (100% over 35bp)
AAGGGGAATCTGACTGTCTAATTAAAACTGGAATTCTCGGGTGCCAAGGA5091110.23200523920401347RNA PCR Primer, Index 1 (100% over 22bp)
GTTACGATCTGCTGAGATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC5075230.23128157713453162RNA PCR Primer, Index 1 (100% over 31bp)
CGTGAATCATCGAATCTTTGAACGCTGGAATTCTCGGGTGCCAAGGAACT4993130.22754022600704674RNA PCR Primer, Index 1 (100% over 25bp)
ATTTGAATGAAGAGCTTTTAGTGGGCCTGGAATTCTCGGGTGCCAAGGAA4878500.22231646133294697RNA PCR Primer, Index 1 (100% over 23bp)
CGCGCAGGTTCGAGTCCTGCAGTTGTCGCCTGGAATTCTCGGGTGCCAAG4821840.21973442777773025No Hit
AGGGGGCATGCCTGTTTGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG4592110.2092654802196657RNA PCR Primer, Index 1 (100% over 29bp)
AGAACTGGTACGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA4424160.20161188799236873RNA PCR Primer, Index 10 (100% over 35bp)
ATTCCAGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT4310920.19645146653467827RNA PCR Primer, Index 10 (100% over 38bp)
AGATGTCCTAAGGGGGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT4191390.19100440563007084RNA PCR Primer, Index 1 (100% over 30bp)
CAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGAACT4083580.18609143285231025RNA PCR Primer, Index 1 (100% over 25bp)
ACTCTACCGTCAGGGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC4074530.18567901838576048RNA PCR Primer, Index 1 (100% over 31bp)
TTCCGGAGAGGGAGCCTGAGAAACGGCTTGGAATTCTCGGGTGCCAAGGA4026280.18348023407514968RNA PCR Primer, Index 1 (100% over 22bp)
GAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGGGTGCCAAGGAACTCC3991170.18188024822757362RNA PCR Primer, Index 1 (100% over 27bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGG3970330.18093055568802693Illumina Small RNA Adapter 2 (100% over 21bp)
TTCCAGGGGGCATGCCTGTTTGAGCTGGAATTCTCGGGTGCCAAGGAACT3950350.18002005391546727RNA PCR Primer, Index 1 (100% over 25bp)
AATAACTTTTCGAATCGCATGGCCTGGAATTCTCGGGTGCCAAGGAACTC3893520.1774302733481717RNA PCR Primer, Index 1 (100% over 26bp)
AGCTGTTGGGACCCGAAAGATGGTGAACTTGGAATTCTCGGGTGCCAAGG3816380.17391495269075172Illumina Small RNA Adapter 2 (100% over 21bp)
ACGGACCAAGGAGTCTAACGTCTTGGAATTCTCGGGTGCCAAGGAACTCC3680940.16774286259688384RNA PCR Primer, Index 1 (100% over 27bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTTGGAATTCTCGGGTGCCAAGGAA3546880.16163366001283244RNA PCR Primer, Index 1 (100% over 23bp)
AATTGGAGGGCAAGTCTGGTGCCAGCTGGAATTCTCGGGTGCCAAGGAAC3507210.15982587195890643RNA PCR Primer, Index 1 (100% over 24bp)
AGTGAGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT3416170.15567711913739335RNA PCR Primer, Index 10 (100% over 39bp)
GTTACGATCTGCTGAGATTAAGCTGGAATTCTCGGGTGCCAAGGAACTCC3386790.15433825316753336RNA PCR Primer, Index 1 (100% over 27bp)
AACGATCAACTTAGAACTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG3380980.15407348763707432RNA PCR Primer, Index 1 (100% over 29bp)
ATGCCTGTTTGAGCGTCTGGAATTCTCGGGGGCCAAGGAACTCCAGTCAC3373870.15374948024954183RNA PCR Primer, Index 1 (96% over 33bp)
ACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3312370.15094688470337472RNA PCR Primer, Index 1 (100% over 33bp)
AGTTTTGGTGGCAGGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3252850.1482345190625964RNA PCR Primer, Index 1 (100% over 32bp)
AAACTATGCCGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG3166100.14428126436942573RNA PCR Primer, Index 10 (100% over 36bp)
ATCGAATCTTTGAACGCACATTGCGCCCCTTGGAATTCTCGGGTGCCAAG3164520.14420926272775184No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATTGGAATTCTCGGGTGCCAAGG3087040.14067844804617416Illumina Small RNA Adapter 2 (100% over 21bp)
ATTCAAATTTCTGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3086140.14063743445281562RNA PCR Primer, Index 1 (100% over 33bp)
AACAACGGATCTCTTGGTTCTCGCTGGAATTCTCGGGTGCCAAGGAACTC3070600.13992926640749143RNA PCR Primer, Index 1 (100% over 26bp)
AGAACTGGCGATGCGGGATGAACCGAACTGGAATTCTCGGGTGCCAAGGA3060450.13946672421572565RNA PCR Primer, Index 1 (100% over 22bp)
AGCGGAACGCTCTGGAAAGTGCGGCCTGGAATTCTCGGGTGCCAAGGAAC2972340.1354514934259243RNA PCR Primer, Index 1 (100% over 24bp)
GGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG2949310.13440200114253845RNA PCR Primer, Index 1 (100% over 29bp)
TGCCAAGGACGTTTTCATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2908460.1325404397106535RNA PCR Primer, Index 1 (100% over 31bp)
GCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA2897100.13202275702115007RNA PCR Primer, Index 10 (100% over 35bp)
CCAGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT2871310.13084748971813137RNA PCR Primer, Index 10 (100% over 41bp)
AAGGTGCCGGAATACACGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG2836400.1292566180024128RNA PCR Primer, Index 1 (100% over 29bp)
GGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCA2828000.12887382446439974RNA PCR Primer, Index 1 (100% over 28bp)
AGGGGCGAAAGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG2773370.12638429934753617RNA PCR Primer, Index 10 (100% over 36bp)
AGTGAAACTGCGAATGGCTCATTTGGAATTCTCGGGTGCCAAGGAACTCC2728690.12434820229058094RNA PCR Primer, Index 1 (100% over 27bp)
ATACGCGAAACTCAGGTGCTGCTGGAATTCTCGGGTGCCAAGGAACTCCA2655870.12102974688128193RNA PCR Primer, Index 1 (100% over 28bp)
AATGACCCACTCGGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2638230.12022588045145449RNA PCR Primer, Index 1 (100% over 33bp)
TTGCTGGCTCCGGTGCGCTTGTGACTGGAATTCTCGGGTGCCAAGGAACT2610510.11896266177601135RNA PCR Primer, Index 1 (100% over 25bp)
ACCGTTTCCCGTCCGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2559220.11662534266116728RNA PCR Primer, Index 1 (100% over 32bp)
TGCCAAGGACGTTTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT2456780.11195708432377932RNA PCR Primer, Index 3 (100% over 34bp)
ATCAGTTTTGGTGGCAGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGT2443560.11135464020800162RNA PCR Primer, Index 1 (100% over 30bp)
AGTCCGGATCGCAGTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2435300.11097822656228877RNA PCR Primer, Index 1 (100% over 31bp)
AATAACTTTTCGAATCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2428830.11068338439670013RNA PCR Primer, Index 1 (100% over 32bp)
TGCCAAGGACGTTTTCATTAATCAAGAACTGGAATTCTCGGGTGCCAAGG2314860.10548969635772996Illumina Small RNA Adapter 2 (100% over 21bp)
ACGGCCTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT2307960.1051752588086478RNA PCR Primer, Index 10 (100% over 38bp)
GAAGGGCCGTCGAAGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2297240.10468674134108827RNA PCR Primer, Index 1 (100% over 32bp)
CGGAGAGGGAGCCTGAGAAACGGCTACCACTGGAATTCTCGGGTGCCAAG2264450.10319247942305869No Hit
AGTTTTGGTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT2264410.10319065659668719RNA PCR Primer, Index 10 (100% over 38bp)
TGCCAAGGACGTTTTCATTAATCTGGAATTCTCGGGTGCCAAGGAACTCC2248940.10248567849751314RNA PCR Primer, Index 1 (100% over 27bp)
AAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAAC2234910.10184632214771272RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph