FastQCFastQC Report
Sun 2 Jul 2023
SRR1190357.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1190357.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220910484
Total Bases11 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGA48833742.2105668828284313RNA PCR Primer, Index 1 (100% over 22bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCAA45978052.081297780326261No Hit
AACGGGGTATTGTAAGCAGTAGAGTAGCCTTGGAATTCTCGGGTGCCAAG33066171.4968130711261307No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCTTGGAATTCTCGGGTGCCAA30176221.365993114206386No Hit
AACTTTTCGAATCGCATGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG22869321.0352301794784895RNA PCR Primer, Index 1 (100% over 29bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGG19387090.8775993628260756Illumina Small RNA Adapter 2 (100% over 21bp)
ACTGTGGTAATTCTAGAGCTAATACATGCTTGGAATTCTCGGGTGCCAAG14224430.6439001781373128No Hit
AGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG14164270.6411769031296858RNA PCR Primer, Index 1 (100% over 29bp)
AATAACTTTTCGAATCGCATGGCCTGGAATTCTCGGGTGCCAAGGAACTC13981010.632881235278992RNA PCR Primer, Index 1 (100% over 26bp)
ATACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTC13965230.6321669187959409RNA PCR Primer, Index 1 (100% over 26bp)
AATGACCCACTCGGCACCTTACGAGAAATCTGGAATTCTCGGGTGCCAAG13362820.6048975022842284No Hit
ACGGGGTATTGTAAGCAGTAGAGTAGCCTTGGAATTCTCGGGTGCCAAGG12911560.5844702236947704Illumina Small RNA Adapter 2 (100% over 21bp)
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT12892950.5836278010236943RNA PCR Primer, Index 1 (100% over 25bp)
AAGGGGAATCTGACTGTCTAATTAAAACTGGAATTCTCGGGTGCCAAGGA11124110.5035573594596805RNA PCR Primer, Index 1 (100% over 22bp)
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAA9998970.45262541727082545No Hit
GAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACTC9805460.44386576057657817RNA PCR Primer, Index 1 (100% over 26bp)
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG9497590.4299293464044015No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGG8725210.39496586318646604Illumina Small RNA Adapter 2 (100% over 21bp)
ATTTGAATGAAGAGCTTTTAGTGGGCCTGGAATTCTCGGGTGCCAAGGAA7983200.3613771449615764RNA PCR Primer, Index 1 (100% over 23bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGAA7794090.35281666396602523RNA PCR Primer, Index 1 (100% over 23bp)
CGGGATAAGGATTGGCTCTAAGGGTCGGTGGAATTCTCGGGTGCCAAGGA7174860.32478585307884256RNA PCR Primer, Index 1 (100% over 22bp)
ATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC6746540.3053970041548594RNA PCR Primer, Index 1 (100% over 33bp)
AAGGATTGGCTCTAAGGGTCGGTGGAATTCTCGGGTGCCAAGGAACTCCA6497410.29411958556027606RNA PCR Primer, Index 1 (100% over 28bp)
AGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGAACTC6436510.29136281282150467RNA PCR Primer, Index 1 (100% over 26bp)
TCGGGATAAGGATTGGCTCTAAGGGTCGGTGGAATTCTCGGGTGCCAAGG6246970.28278286692812643Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGGGTCTTATGGCTGGAAGAGGCCAGCATGGAATTCTCGGGTGCCAAG6232410.28212377643425923No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCAAG6085490.27547311878597847No Hit
TACATGGTATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAA5650120.25576513607203905No Hit
TACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCC5433940.2459792718574642RNA PCR Primer, Index 1 (100% over 27bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA5422410.24545734099247185No Hit
TATACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACT5391050.24403776146721948RNA PCR Primer, Index 1 (100% over 25bp)
ATAACTGTGGTAATTCTAGAGCTAATACATGCTTGGAATTCTCGGGTGCC5134480.23242355487302271No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCTGGAATTCTCGGGTGCCAAGG5079810.22994879681672328Illumina Small RNA Adapter 2 (100% over 21bp)
CACGTTCTAGCATTCAAGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG4859050.21995560880668752RNA PCR Primer, Index 1 (100% over 29bp)
ATTCATAATAACTTTTCGAATCGCATGGCCTGGAATTCTCGGGTGCCAAG4765450.21571859848896988No Hit
AATGACCCACTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG4760270.21548411437095943RNA PCR Primer, Index 13 (100% over 35bp)
ACGGGGAAACTCACCAGGTCCAGACTGGAATTCTCGGGTGCCAAGGAACT4647330.21037163632306377RNA PCR Primer, Index 1 (100% over 25bp)
CCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGAAC4619400.20910732330838583RNA PCR Primer, Index 1 (100% over 24bp)
AAGCTGGTAAGAGCCTGACCGAGTTGGAATTCTCGGGTGCCAAGGAACTC4471530.20241366181606846RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTGGTTATCCACTTCTTAGAGGGACTTGGAATTCTCGGGTGCCAAGG4366070.19763978245595626Illumina Small RNA Adapter 2 (100% over 21bp)
TGCCAAGGACGTTTTCATTAATCAAGAACTGGAATTCTCGGGTGCCAAGG4203250.19026937626011448Illumina Small RNA Adapter 2 (100% over 21bp)
AACGGGCTTGGCAGAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4188200.18958810483616523RNA PCR Primer, Index 1 (100% over 32bp)
AGCTGTTGGGACCCGAAAGATGGTGAACTTGGAATTCTCGGGTGCCAAGG4149030.1878149884457272Illumina Small RNA Adapter 2 (100% over 21bp)
AGAACTGGCGATGCGGGATGAACCGAACTGGAATTCTCGGGTGCCAAGGA4107550.18593730481347368RNA PCR Primer, Index 1 (100% over 22bp)
CAACGGGGTATTGTAAGCAGTAGAGTAGCCTTGGAATTCTCGGGTGCCAA4034650.18263732562371282No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATTGGAATTCTCGGGTGCCAAGG3861470.17479795119185018Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTAGTTAAGCTGGTAAGAGCCTGACTGGAATTCTCGGGTGCCAAGG3857030.17459696480498407Illumina Small RNA Adapter 2 (100% over 21bp)
TTGCCAAGGACGTTTTCATTAATCAAGAACTGGAATTCTCGGGTGCCAAG3726220.16867556181715668No Hit
TAGTACCGATTGAATGGCTTAGTGAGGCCTTGGAATTCTCGGGTGCCAAG3653150.16536788720267348No Hit
AATGACCCACTCGGCACCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT3635670.16457661647239885RNA PCR Primer, Index 1 (100% over 30bp)
ATTGTAAGCAGTAGAGTAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA3617620.16375954343570223RNA PCR Primer, Index 1 (100% over 28bp)
ATCGAATCTTTGAACGCACATTGCGCCCCTTGGAATTCTCGGGTGCCAAG3518270.15926224669355213No Hit
TTGCTGGCTCCGGTGCGCTTGTGACTGGAATTCTCGGGTGCCAAGGAACT3515080.15911784431199744RNA PCR Primer, Index 1 (100% over 25bp)
CGTGAATCATCGAATCTTTGAACGCTGGAATTCTCGGGTGCCAAGGAACT3479490.15750678451277125RNA PCR Primer, Index 1 (100% over 25bp)
GAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3410470.15438244207549698RNA PCR Primer, Index 1 (100% over 32bp)
AAGCAGTAGAGTAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3394660.15366676757631836RNA PCR Primer, Index 1 (100% over 33bp)
AACGATCAACTTAGAACTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG3386900.1533154940713452RNA PCR Primer, Index 1 (100% over 29bp)
TTCCAGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA3339220.15115715377274713RNA PCR Primer, Index 13 (100% over 39bp)
AGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCAAGGAA3327280.15061666335401266RNA PCR Primer, Index 1 (100% over 23bp)
ACCTATACTCTACCGTCAGGGTTGATGGAATTCTCGGGTGCCAAGGAACT3299910.14937769997371422RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3269180.14798663878713877RNA PCR Primer, Index 1 (100% over 31bp)
ATAATAACTTTTCGAATCGCATGGCCTGGAATTCTCGGGTGCCAAGGAAC3225070.14598990240771006RNA PCR Primer, Index 1 (100% over 24bp)
AAACTTTCAACAACGGATCTCTTGGTTCTCGTGGAATTCTCGGGTGCCAA3219500.1457377640800425No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCTGGAATTCTCGGGTGCCA3219040.14571694116608788No Hit
ACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA3163760.1432145701151965RNA PCR Primer, Index 1 (100% over 28bp)
AACGGGGTATTGTAAGCAGTAGAGTAGCCTGGAATTCTCGGGTGCCAAGG3123610.14139709186459434Illumina Small RNA Adapter 2 (100% over 21bp)
GAGATGGGGAAGCTCCGTTTCAAAGGCCTGTGGAATTCTCGGGTGCCAAG3089560.13985574355991182No Hit
AATCTTTGAACGCACATTGCGCCCCTTGGTTGGAATTCTCGGGTGCCAAG2976570.1347410021518037No Hit
AGAATTCGGTAAGCGTTGGATTGTTCTGGAATTCTCGGGTGCCAAGGAAC2904210.13146546725233738RNA PCR Primer, Index 1 (100% over 24bp)
TACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTGGAATTCT2872770.13004226635074503No Hit
GTGAATCATCGAATCTTTGAACGCTGGAATTCTCGGGTGCCAAGGAACTC2844030.1287412869006253RNA PCR Primer, Index 1 (100% over 26bp)
CGGCAAAAGCTCAAATTTGAAATCTGGTACCTGGAATTCTCGGGTGCCAA2810620.12722890960666222No Hit
TAACGATCAACTTAGAACTGGTTGGAATTCTCGGGTGCCAAGGAACTCCA2790490.12631768078512742RNA PCR Primer, Index 1 (100% over 28bp)
AAGGCTGAAACTTAAAGGAATTGACTGGAATTCTCGGGTGCCAAGGAACT2725530.12337712319710457RNA PCR Primer, Index 1 (100% over 25bp)
GGGCCAGCATCAGTTTTGGTGGCAGGATTGGAATTCTCGGGTGCCAAGGA2638840.11945290926074835RNA PCR Primer, Index 1 (100% over 22bp)
AACAACGGATCTCTTGGTTCTCGCTGGAATTCTCGGGTGCCAAGGAACTC2628950.11900521661072454RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGTATTGTAAGCAGTAGAGTAGCCTTGGAATTCTCGGGTGCCAAGGA2620720.11863266751975429RNA PCR Primer, Index 1 (100% over 22bp)
AAGGGGAATCTGACTGTCTAATTAAAACATTGGAATTCTCGGGTGCCAAG2610630.11817592142888067No Hit
AATTGCGATAACGAACGAGACCTTAACCTTGGAATTCTCGGGTGCCAAGG2536920.114839275803678Illumina Small RNA Adapter 2 (100% over 21bp)
AGATGTCCTAAGGGGGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2506740.11347311157943957RNA PCR Primer, Index 1 (100% over 30bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTTGGAATTCTCGGGTGCCAAGGAA2467370.11169094174815171RNA PCR Primer, Index 1 (100% over 23bp)
ACTCTACCGTCAGGGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC2460120.11136275451734559RNA PCR Primer, Index 1 (100% over 31bp)
CTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCCAAG2452990.11103999935104937No Hit
GCGGCAAAAGCTCAAATTTGAAATCTGGTATGGAATTCTCGGGTGCCAAG2440930.11049407686780496No Hit
AAGGGGAATCTGACTGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2437820.11035329586259021RNA PCR Primer, Index 1 (100% over 31bp)
AGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2436010.1102713622228993RNA PCR Primer, Index 1 (100% over 30bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCAAGG2407260.10896993010073709Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGGGTCTTATGGCTGGAAGAGGCCAGCTGGAATTCTCGGGTGCCAAGG2394860.10840861676804801Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGCAGGTTCGAGTCCTGCAGTTGTCGCCTGGAATTCTCGGGTGCCAAG2394490.10839186790247582No Hit
TGGCGATGGTTCATTCAAATTTCTGCCCTTGGAATTCTCGGGTGCCAAGG2377960.10764360101623788Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGGAGAATTTGGACAAACTTGGTCATTTTGGAATTCTCGGGTGCCAAG2328000.10538205149195183No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGTTTGGAATTCTCGGGTGCCAAG2309130.10452785934777092No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCTGGAATTCTCGGGTGCCAAG2244460.10160042924898033No Hit
AATGAAAGTGAACGTAGGTTGGGGCCTCTGGAATTCTCGGGTGCCAAGGA2234840.10116495874410379RNA PCR Primer, Index 1 (100% over 22bp)
GTTACGATCTGCTGAGATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2230020.10094677081962304RNA PCR Primer, Index 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)