FastQCFastQC Report
Sun 2 Jul 2023
SRR1190358.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1190358.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234945553
Total Bases11.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAAC33389551.4211611828209407RNA PCR Primer, Index 1 (100% over 24bp)
ATACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTC32487061.3827484532129024RNA PCR Primer, Index 1 (100% over 26bp)
TATACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACT24194921.0298096597725346RNA PCR Primer, Index 1 (100% over 25bp)
CAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAA12322150.5244683222414513RNA PCR Primer, Index 1 (100% over 23bp)
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGGAATTCTCGGGTGCCAAG12090840.5146230624760963No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGA11819100.5030569784821592RNA PCR Primer, Index 1 (100% over 22bp)
AAGGATTGGCTCTAAGGGTCGGTGGAATTCTCGGGTGCCAAGGAACTCCA11333560.4823909137790746RNA PCR Primer, Index 1 (100% over 28bp)
GCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCAAGG10747690.4574544979789424Illumina Small RNA Adapter 2 (100% over 21bp)
AACTTTTCGAATCGCATGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG10473440.4457815807222365RNA PCR Primer, Index 1 (100% over 29bp)
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGGAATTCTCGGGTGCCAAGG10282510.4376550170328186Illumina Small RNA Adapter 2 (100% over 21bp)
ATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC9251510.39377250949712594RNA PCR Primer, Index 1 (100% over 33bp)
ACTTGCGTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAG8910780.37927000048389936No Hit
AGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT8792560.3742381963705438RNA PCR Primer, Index 1 (100% over 30bp)
AAGGATTGGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG7994620.340275433942774RNA PCR Primer, Index 12 (100% over 37bp)
CTTGCGTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGG7820370.33285882197566Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTGGTAATTCTAGAGCTAATACATGCTTGGAATTCTCGGGTGCCAA7400670.3149951086752427No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCAA7367520.313584143471743No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA7155900.3045769502179086No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGGA6988620.2974570027294792RNA PCR Primer, Index 1 (100% over 22bp)
AAGCTGGTAAGAGCCTGACCGAGTTGGAATTCTCGGGTGCCAAGGAACTC6948200.29573660413142616RNA PCR Primer, Index 1 (100% over 26bp)
CGCGCAGGTTCGAGTCCTGCAGTTGTCGCCTGGAATTCTCGGGTGCCAAG6873060.29253841633682676No Hit
ACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA6750940.2873406163171771RNA PCR Primer, Index 1 (100% over 28bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGG6738940.2868298596824261Illumina Small RNA Adapter 2 (100% over 21bp)
ATTTGAATGAAGAGCTTTTAGTGGGCCTGGAATTCTCGGGTGCCAAGGAA6679680.2843075731678139RNA PCR Primer, Index 1 (100% over 23bp)
GCTCTGCTAGGCGGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC6624040.28193936490468496RNA PCR Primer, Index 1 (100% over 33bp)
AGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG6345570.2700868315647583RNA PCR Primer, Index 1 (100% over 29bp)
AGTTGAACTTTGGGCCCGGTTGGCTGGAATTCTCGGGTGCCAAGGAACTC6343050.2699795726714606RNA PCR Primer, Index 1 (100% over 26bp)
AATAACTTTTCGAATCGCATGGCCTGGAATTCTCGGGTGCCAAGGAACTC6091600.2592770930207817RNA PCR Primer, Index 1 (100% over 26bp)
AAGGGGAATCTGACTGTCTAATTAAAACTGGAATTCTCGGGTGCCAAGGA6049650.2574915729517979RNA PCR Primer, Index 1 (100% over 22bp)
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT6002910.2555021758594426RNA PCR Primer, Index 1 (100% over 25bp)
AAGGGGAATCTGACTGTCTAATTAAAACATTGGAATTCTCGGGTGCCAAG5993150.25508676046317846No Hit
TGCGTGCCTTGTTGTAGACGGCCTTGGTTGGAATTCTCGGGTGCCAAGGA5967410.2539911874816375RNA PCR Primer, Index 1 (100% over 22bp)
TACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCC5933070.2525295722451916RNA PCR Primer, Index 1 (100% over 27bp)
AAGGGGAATCTGACTGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC5616050.2390362332161273RNA PCR Primer, Index 1 (100% over 31bp)
TGCGTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGGAA5573620.23723028288175346RNA PCR Primer, Index 1 (100% over 23bp)
AGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG5564670.23684934355833498RNA PCR Primer, Index 1 (100% over 29bp)
AACAACGGATCTCTTGGTTCTCGCTGGAATTCTCGGGTGCCAAGGAACTC5415040.2304806339535186RNA PCR Primer, Index 1 (100% over 26bp)
AGATGTCCTAAGGGGGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT5358310.2280660319627331RNA PCR Primer, Index 1 (100% over 30bp)
AGGGTGAGAATCCCGTGTGGCGAGGAGTGTGGAATTCTCGGGTGCCAAGG5321690.22650737296568452Illumina Small RNA Adapter 2 (100% over 21bp)
ATAGCTGTTGGGACCCGAAAGATGGTGAACTTGGAATTCTCGGGTGCCAA5123230.2180603094879604No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAA4795890.2041277197530102No Hit
TCCAACGGGGCCTTTCCTTCTGGCTTGGAATTCTCGGGTGCCAAGGAACT4786440.20372549890314376RNA PCR Primer, Index 1 (100% over 25bp)
AGCTGTTGGGACCCGAAAGATGGTGAACTTGGAATTCTCGGGTGCCAAGG4759280.20256948638649055Illumina Small RNA Adapter 2 (100% over 21bp)
AATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC4730150.20132962465563242RNA PCR Primer, Index 2 (100% over 34bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCTGGAATTCTCGGGTGCCAAGG4507480.1918521096672981Illumina Small RNA Adapter 2 (100% over 21bp)
AGAACTGGCGATGCGGGATGAACCGAACGTTGGAATTCTCGGGTGCCAAG4434100.18872883284579556No Hit
AATGACCCACTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT4344770.18492667533060309RNA PCR Primer, Index 12 (100% over 35bp)
AATGACCCACTCGGCACCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT4113300.17507460547678466RNA PCR Primer, Index 1 (100% over 30bp)
AACTGTGGTAATTCTAGAGCTAATACATGCTTTGGAATTCTCGGGTGCCA3932370.167373672316326No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG3832040.1631033212192784No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGTTGGAATTCTCGGGTGCCAAGG3811800.162241845028665Illumina Small RNA Adapter 2 (100% over 21bp)
AACGAACGAGACCTTAACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT3683110.15676440575148914RNA PCR Primer, Index 1 (100% over 30bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGG3670200.15621491673860283Illumina Small RNA Adapter 2 (100% over 21bp)
AGTTGAACTTTGGGCCCGGTTGGTGGAATTCTCGGGTGCCAAGGAACTCC3576740.15223697381495022RNA PCR Primer, Index 1 (100% over 27bp)
ATTGCGATGGTCAGAAAGTGATGTTGACGCTGGAATTCTCGGGTGCCAAG3506800.14926011389540964No Hit
TACTTGCGTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAA3432060.14607895132196863No Hit
AATGACCCACTCGGCACCTTACGAGAAATCTGGAATTCTCGGGTGCCAAG3343370.14230403416062956No Hit
CCGTGAATCATCGAATCTTTGAACGCTGGAATTCTCGGGTGCCAAGGAAC3229230.13744588730308932RNA PCR Primer, Index 1 (100% over 24bp)
CGTGAATCATCGAATCTTTGAACGCTGGAATTCTCGGGTGCCAAGGAACT3189790.13576720049687427RNA PCR Primer, Index 1 (100% over 25bp)
ACGCGAAACTCAGGTGCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT3163420.13464481279200888RNA PCR Primer, Index 1 (100% over 30bp)
AAGGTGCCGGAATACACGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG3145650.1338884673420484RNA PCR Primer, Index 1 (100% over 29bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGAA3099820.13193780262782842RNA PCR Primer, Index 1 (100% over 23bp)
TGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCAAG3050990.1298594487549207No Hit
AACGATCAACTTAGAACTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG3013200.1282509909859839RNA PCR Primer, Index 1 (100% over 29bp)
ATTCCAGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT2879190.12254711626740176RNA PCR Primer, Index 12 (100% over 38bp)
AACGGGGCCTTTCCTTCTGGCTAACCTTGGAATTCTCGGGTGCCAAGGAA2846240.12114466367448122RNA PCR Primer, Index 1 (100% over 23bp)
TTAACGATCAACTTAGAACTGGTTGGAATTCTCGGGTGCCAAGGAACTCC2826150.12028957194180219RNA PCR Primer, Index 1 (100% over 27bp)
AACGGGGCCTTTCCTTCTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2819020.1199860973746543RNA PCR Primer, Index 1 (100% over 28bp)
TTCCAGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA2772520.11800691541499404RNA PCR Primer, Index 12 (100% over 39bp)
AGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGAACTC2755160.11726802081672089RNA PCR Primer, Index 1 (100% over 26bp)
AGAACTGGTACGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT2720910.11581023625503566RNA PCR Primer, Index 12 (100% over 35bp)
ACGGGGAAACTCACCAGGTCCAGACTGGAATTCTCGGGTGCCAAGGAACT2640520.11238859243273269RNA PCR Primer, Index 1 (100% over 25bp)
AAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGAA2601920.11074565859095023RNA PCR Primer, Index 1 (100% over 23bp)
AATAACTTTTCGAATCGCATTGGAATTCTCGGGTGCCAAGGAACTCCAGT2600560.11068777283901178RNA PCR Primer, Index 1 (100% over 30bp)
AATTTGGAGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT2525410.10748915941388344RNA PCR Primer, Index 12 (100% over 36bp)
ATAGGAATGTAGCTTGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2514000.107003514980341RNA PCR Primer, Index 1 (100% over 31bp)
AGGATAAATCCATAGGAATGTAGCTTGCCTTGGAATTCTCGGGTGCCAAG2477300.10544145093906077No Hit
AGAACTGGCGATGCGGGATGAACCGAACTGGAATTCTCGGGTGCCAAGGA2450040.104281182117118RNA PCR Primer, Index 1 (100% over 22bp)
TGGGCCAGCATCAGTTTTGGTGGCAGGATTGGAATTCTCGGGTGCCAAGG2417380.10289107280953728Illumina Small RNA Adapter 2 (100% over 21bp)
ACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2400110.10215600888602476RNA PCR Primer, Index 1 (100% over 32bp)
AAAAAGCTCGTAGTTGAACTTTGGGCCCGTGGAATTCTCGGGTGCCAAGG2383740.10145925171011856Illumina Small RNA Adapter 2 (100% over 21bp)
GAGATGGGGAAGCTCCGTTTCAAAGGCCTGTGGAATTCTCGGGTGCCAAG2369650.10085953829481506No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)